A kind of interrhizobia bacteria strain with phosphorus dissolving characteristic and application thereof
A technology of intermediate rhizobia and strains, applied in the fields of application, bacteria, organic fertilizers, etc., can solve the problems of rare research and development reports on phosphorus-solubilizing bacteria, and achieve the effects of improving growth conditions, high-efficiency phosphorus-solubilizing characteristics, and increasing available phosphorus content
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Embodiment 1
[0024] Screening and Identification of Mesorhizobium.sp Strain YTR-M3
[0025] 1. Screening process
[0026] (1) Weigh 1g of rhizosphere soil from the high-fertilizer treatment of Jiangxi Yingtan Red Soil Experiment Station's long-term positioning test site and put it into a centrifuge tube, add 9mL of 0.9% normal saline to make 10 -1 The bacterial suspension was placed in a shaking box at 180r / min for 10 minutes, and then left to stand for 1 hour after taking it out.
[0027] (2) Draw 5 μL of the static soil suspension under sterile conditions and add 45 μL sterile water to dilute to 10 -2 bacterial suspension. The bacterial suspension was diluted to 10 -3 、10 -4 、10 -5 , respectively take 5mL 10 -5 The bacterial suspension was added to 50mL and 500mL of LB medium to make 10 -6 and 10 -7 culture medium, the 10 -6 and 10 -7 Add 250 μL of culture solution to a 96-well plate, and add three plates for each concentration. Finally, the 96-well plate was placed in a micro...
Embodiment 2
[0031] Phosphorus Solubilizing Properties of Mesorhizobium.sp Strain YTR-M3
[0032] (1) The strain YTR-M3 was stored in a glycerol tube, and after activation, it was streaked on LB solid medium and stored for later use.
[0033] (2) Prepare Montina organophosphate (lecithin), aluminum phosphate, and iron phosphate medium plates. Each plate is divided into 6 areas, and 6 μL points are drawn in the center of each area in turn. Cultivate for 4 days, observe and record the diameter of the phosphorus-dissolving circle (unit D / cm), the diameter of the colony (unit d / cm), and calculate the D / d ratio to determine the phosphorus-dissolving ability and efficiency of the strain for different insoluble phosphorus. The formula of the culture medium is as follows: ①Mengkina organic phosphorus (lecithin) bacterial culture medium (1L) glucose 10.0g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, MgSO 4 ·7H 2 O 0.3g, FeSO 4 0.03g, MnSO 4 ·H 2 O 0.03g, KCl 0.3g, CaCO 3 1.0g, lecithin 0.3g, agar 2...
Embodiment 3
[0038] Corn pot experiment
[0039] 1. A total of 3 treatments were set up in the experiment, which were control, wood vinegar (wood vinegar compound water-soluble fertilizer containing amino acids), wood vinegar + bacterial agent (YTR-M3), and 4 replicates for each treatment.
[0040] 2. Dosage of soil and fertilizer: about 500g of soil in each 10×10(cm) pot, add 15mL of wood vinegar (1:800 dilution) to each pot as base fertilizer. Then apply every other week as a root drip and foliar spray.
[0041] 3. Seedling cultivation: 1) Disinfect seeds, select appropriate amount of seeds, first disinfect with 70% alcohol for 1 min, then disinfect with 1% sodium hypochlorite for 15 min, and finally rinse with sterile water five times. Put the sterilized seeds on sterile water, place them in a light incubator at 28° C. and wait for them to germinate; 2) select seedlings with consistent growth and transplant them into red soil on the fifth day after the seeds germinate.
[0042] 4. Add...
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