Biosynthesis method of dendritic glucosyl nanoparticles

A nanoparticle, glucosyl technology, applied in the field of biosynthesis of dendritic glucosyl nanoparticles, can solve the problem of few research reports on glucosyl nanoparticle polymers, difficulty in controlling the degree of polymerization and branching, and random structure etc. to achieve the effects of low cost, easy operation, and controllable reaction conditions

Pending Publication Date: 2020-01-21
JIANGNAN UNIV
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AI-Extracted Technical Summary

Problems solved by technology

The degree of polymerization and branching of dendrimer synthesized by chemical method is difficult to control, its structure is random, and there are many side reactions in the reaction, the product needs to be purified, the pre...
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Abstract

The invention discloses a biosynthesis method of dendritic glucosyl nanoparticles, and belongs to the technical field of carbohydrate deep processing. According to the biosynthesis method of the dendritic glucosyl nanoparticles, cyclodextrin is taken as the raw material, and the dendritic glucosyl nanoparticles are prepared by a one-pot synthesis technology based on the cooperative catalysis of invitro polysaccharide enzyme preparation. The biosynthesis method is simple in process, controllable in reaction conditions and continuous in production, and further has the characteristics of low cost, environmental friendliness and the like. The diameter size of the dendrimer glucosyl nanoparticles is 20-100nm, the absolute molecular weight is greater than 1.0 *10<5>g/mol, the polydispersity coefficient is less than 1.3, and the branch density is 6-12%. The dendrimer glucosyl nanoparticles can be widely used as carriers in industries of nutrition food, medicine, cosmetics and the like.

Application Domain

NanotechnologyFermentation

Technology Topic

NutritionEnzyme +10

Image

  • Biosynthesis method of dendritic glucosyl nanoparticles
  • Biosynthesis method of dendritic glucosyl nanoparticles
  • Biosynthesis method of dendritic glucosyl nanoparticles

Examples

  • Experimental program(3)
  • Comparison scheme(3)

Example Embodiment

[0028] Example 1:
[0029] Weigh 10g β-cyclodextrin and dissolve it in 1000mL phosphate buffer (pH 7.0), place it in a 70℃ water bath for 30 minutes, and then add 100U in vitro polysaccharidase preparation (New Pullulanase: Hydrolase family 13, 1, 4-α-glucan branching enzyme = 1:1) Constant temperature reaction for 6 hours, after the reaction is completed, the enzyme activity is inactivated by heating, centrifuged, and the supernatant obtained is vacuum dried to obtain the target product. The average size of the dendritic glucosyl nanoparticles is 35nm and the absolute molecular weight is 4.2×10 6 g/mol, polydispersity coefficient 1.0, branch density 7.3%.

Example Embodiment

[0030] Example 2:
[0031] Weigh 10g of large-membered cyclodextrin and dissolve it in 50mL of phosphate buffer (pH 7.0), place it in a 70℃ water bath for 30 minutes, and then add 200U in vitro polysaccharidase preparation (cyclomaltodextrinase: 1 of hydrolase family 13 , 4-α-glucan branching enzyme = 10:1) Constant temperature reaction for 24 hours, after the reaction is completed, heat inactivation of the enzyme, centrifugal treatment, vacuum drying of the obtained supernatant to obtain the target product. The average size of dendritic glucosyl nanoparticles is 78nm, and the absolute molecular weight is 1.9×10 7 g/mol, polydispersity coefficient 1.2, branch density 6.7%.

Example Embodiment

[0032] Example 3:
[0033] Weigh 10g of γ-cyclodextrin and dissolve it in 250mL phosphate buffer (pH 7.0), place it in a 70℃ water bath for 30 minutes, and then add 50U of in vitro polysaccharidase preparation (maltose glucosylase: hydrolase family 57 1, 4-α-glucan branching enzyme=1:10) Constant temperature reaction for 2h, heating to inactivate the enzyme after the reaction, centrifugation treatment, vacuum drying treatment of the obtained supernatant to obtain the target product. The average size of the dendritic glucosyl nanoparticles is 41nm, and the absolute molecular weight is 8.9×10 5 g/mol, polydispersity coefficient 1.1, branch density 6.1%.

PUM

PropertyMeasurementUnit
Average size78.0nm
Absolute molecular weight19000000.0

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