Biosynthesis method of dendritic glucosyl nanoparticles

A nanoparticle, glucosyl technology, applied in the field of biosynthesis of dendritic glucosyl nanoparticles, can solve the problem of few research reports on glucosyl nanoparticle polymers, difficulty in controlling the degree of polymerization and branching, and random structure etc. to achieve the effects of low cost, easy operation, and controllable reaction conditions

Pending Publication Date: 2020-01-21

JIANGNAN UNIV

9 Cites 0 Cited by

AI-Extracted Technical Summary

Problems solved by technology

The degree of polymerization and branching of dendrimer synthesized by chemical method is difficult to control, its structure is random, and there are many side reactions in the reaction, the product needs to be purified, the pre...

Abstract

The invention discloses a biosynthesis method of dendritic glucosyl nanoparticles, and belongs to the technical field of carbohydrate deep processing. According to the biosynthesis method of the dendritic glucosyl nanoparticles, cyclodextrin is taken as the raw material, and the dendritic glucosyl nanoparticles are prepared by a one-pot synthesis technology based on the cooperative catalysis of invitro polysaccharide enzyme preparation. The biosynthesis method is simple in process, controllable in reaction conditions and continuous in production, and further has the characteristics of low cost, environmental friendliness and the like. The diameter size of the dendrimer glucosyl nanoparticles is 20-100nm, the absolute molecular weight is greater than 1.0 *10<5>g/mol, the polydispersity coefficient is less than 1.3, and the branch density is 6-12%. The dendrimer glucosyl nanoparticles can be widely used as carriers in industries of nutrition food, medicine, cosmetics and the like.

Application Domain

NanotechnologyFermentation

Technology Topic

NutritionEnzyme +10

Image

Examples

Experimental program(3)
Comparison scheme(3)

Example Embodiment

[0028] Example 1:

[0029] Weigh 10g β-cyclodextrin and dissolve it in 1000mL phosphate buffer (pH 7.0), place it in a 70℃ water bath for 30 minutes, and then add 100U in vitro polysaccharidase preparation (New Pullulanase: Hydrolase family 13, 1, 4-α-glucan branching enzyme = 1:1) Constant temperature reaction for 6 hours, after the reaction is completed, the enzyme activity is inactivated by heating, centrifuged, and the supernatant obtained is vacuum dried to obtain the target product. The average size of the dendritic glucosyl nanoparticles is 35nm and the absolute molecular weight is 4.2×10 6 g/mol, polydispersity coefficient 1.0, branch density 7.3%.

Example Embodiment

[0030] Example 2:

[0031] Weigh 10g of large-membered cyclodextrin and dissolve it in 50mL of phosphate buffer (pH 7.0), place it in a 70℃ water bath for 30 minutes, and then add 200U in vitro polysaccharidase preparation (cyclomaltodextrinase: 1 of hydrolase family 13 , 4-α-glucan branching enzyme = 10:1) Constant temperature reaction for 24 hours, after the reaction is completed, heat inactivation of the enzyme, centrifugal treatment, vacuum drying of the obtained supernatant to obtain the target product. The average size of dendritic glucosyl nanoparticles is 78nm, and the absolute molecular weight is 1.9×10 7 g/mol, polydispersity coefficient 1.2, branch density 6.7%.

Example Embodiment

[0032] Example 3:

[0033] Weigh 10g of γ-cyclodextrin and dissolve it in 250mL phosphate buffer (pH 7.0), place it in a 70℃ water bath for 30 minutes, and then add 50U of in vitro polysaccharidase preparation (maltose glucosylase: hydrolase family 57 1, 4-α-glucan branching enzyme=1:10) Constant temperature reaction for 2h, heating to inactivate the enzyme after the reaction, centrifugation treatment, vacuum drying treatment of the obtained supernatant to obtain the target product. The average size of the dendritic glucosyl nanoparticles is 41nm, and the absolute molecular weight is 8.9×10 5 g/mol, polydispersity coefficient 1.1, branch density 6.1%.

PUM

Property	Measurement	Unit
Average size	78.0	nm
Absolute molecular weight	19000000.0

Description & Claims & Application Information

We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.

Classification and recommendation of technical efficacy words

Simple process
low cost

Biosynthesis method of dendritic glucosyl nanoparticles

AI-Extracted Technical Summary

Problems solved by technology

Abstract

Image

Examples

Example Embodiment

Example Embodiment

Example Embodiment

PUM

Description & Claims & Application Information

Similar technology patents

Fuel cell stack with separator of a laminate structure

Novel anti-reflection conductive film

Image processing system, mask fabrication method, and program

Preparation of antibacterial nano filter membrance

Preparation method of Apremilast

Classification and recommendation of technical efficacy words

Multimedia data recording apparatus, monitor system, and multimedia data recording method

Simultaneously desulfurization and denitration wet ammonia flue gas cleaning technology and system thereof

Method for recycling metal oxide from waste flue gas denitration catalyst

Method for removing COS from a stream of hydrocarbon fluid and wash liquid for use in a method of this type

Method for preparing graphite alkyne film

Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like

System and method for transmitting wireless digital service signals via power transmission lines

System and method for determination of position

Adaptive antenna optimization network

Antenna device and method for attaching the same