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Biosynthesis method of dendritic glucosyl nanoparticles

A nanoparticle, glucosyl technology, applied in the field of biosynthesis of dendritic glucosyl nanoparticles, can solve the problem of few research reports on glucosyl nanoparticle polymers, difficulty in controlling the degree of polymerization and branching, and random structure etc. to achieve the effects of low cost, easy operation, and controllable reaction conditions

Pending Publication Date: 2020-01-21
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The degree of polymerization and branching of dendrimer synthesized by chemical method is difficult to control, its structure is random, and there are many side reactions in the reaction, the product needs to be purified, the preparation cost is high, and the price is expensive
However, there are few reports on the research on glucosyl nanoparticle polymers, and there has been no report on the preparation of dendritic glucosyl nanoparticles by cyclodextrin

Method used

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  • Biosynthesis method of dendritic glucosyl nanoparticles
  • Biosynthesis method of dendritic glucosyl nanoparticles
  • Biosynthesis method of dendritic glucosyl nanoparticles

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Weigh 10g of β-cyclodextrin and dissolve it in 1000mL of phosphate buffer (pH 7.0), place it in a water bath at 70°C for 30 minutes, and then add 100U of in vitro polysaccharase preparation (new pullulanase: 1 of the carbohydrase family 13, 4-α-glucan branching enzyme = 1:1) react at constant temperature for 6 hours, heat inactivate the enzyme after the reaction, centrifuge, and vacuum-dry the supernatant to obtain the target product. Dendritic glucosyl nanoparticles have an average size of 35nm and an absolute molecular weight of 4.2×10 6 g / mol, polydispersity coefficient 1.0, branch density 7.3%.

Embodiment 2

[0031] Weigh 10g macrocyclodextrin and dissolve it in 50mL phosphate buffer (pH 7.0), place it in a 70°C water bath for 30 minutes, and then add 200U in vitro polysaccharide enzyme preparation (cyclomatodextrinase: 1 of the carbohydrase family 13 , 4-α-glucan branching enzyme = 10:1) constant temperature reaction for 24 hours, after the reaction was completed, the enzyme was inactivated by heating, centrifuged, and the obtained supernatant was vacuum-dried to obtain the target product. Dendritic glucosyl nanoparticles have an average size of 78nm and an absolute molecular weight of 1.9×10 7 g / mol, polydispersity coefficient 1.2, branch density 6.7%.

Embodiment 3

[0033] Weigh 10 g of γ-cyclodextrin and dissolve it in 250 mL of phosphate buffer (pH 7.0), place it in a water bath at 70°C for 30 min, and then add 50 U of in vitro polysaccharide enzyme preparation (maltose glucosylase: 1 of the carbohydrase family 57, 4-α-glucan branching enzyme = 1:10) constant temperature reaction for 2 hours, heat inactivation and centrifugation after the reaction, and vacuum-dry the obtained supernatant to obtain the target product. Dendritic glucosyl nanoparticles have an average size of 41nm and an absolute molecular weight of 8.9×10 5 g / mol, polydispersity coefficient 1.1, branch density 6.1%.

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Abstract

The invention discloses a biosynthesis method of dendritic glucosyl nanoparticles, and belongs to the technical field of carbohydrate deep processing. According to the biosynthesis method of the dendritic glucosyl nanoparticles, cyclodextrin is taken as the raw material, and the dendritic glucosyl nanoparticles are prepared by a one-pot synthesis technology based on the cooperative catalysis of invitro polysaccharide enzyme preparation. The biosynthesis method is simple in process, controllable in reaction conditions and continuous in production, and further has the characteristics of low cost, environmental friendliness and the like. The diameter size of the dendrimer glucosyl nanoparticles is 20-100nm, the absolute molecular weight is greater than 1.0 *10<5>g / mol, the polydispersity coefficient is less than 1.3, and the branch density is 6-12%. The dendrimer glucosyl nanoparticles can be widely used as carriers in industries of nutrition food, medicine, cosmetics and the like.

Description

technical field [0001] The invention belongs to the technical field of carbohydrate deep processing, and in particular relates to a biosynthesis method of dendritic glucosyl nanoparticles. Background technique [0002] In recent years, in order to improve the bioavailability of active substances, nanoscale delivery systems for embedding, protecting and delivering fat-soluble bioactive ingredients have emerged as the times require. Due to its small particle size, large relative surface area, high reactivity, and strong adsorption, the nano-transport system has great potential in the transport of functional nutrients, and its manufacturing and property research technologies are gradually becoming mature. Of course, the application of nanoscale transport systems also needs to meet certain requirements. In the face of processing, storage, transportation, heat treatment, drying, light, mechanical external force, etc., the nanotransport system should have certain physical and che...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/18C12P19/04B82Y40/00
CPCB82Y40/00C12P19/04C12P19/14C12P19/18
Inventor 缪铭袁嘉辉王艳丽陈一枚张涛金征宇
Owner JIANGNAN UNIV
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