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Low-crystal fibroin bracket and preparation method thereof

A protein scaffold and silk protein technology, applied in the field of biomedicine, can solve the problem of inability to prepare low crystallinity water-insoluble silk protein scaffold, etc., and achieve the effect of complete and uniform porous structure, mild conditions and low crystallinity

Active Publication Date: 2020-01-24
上海丝波敦生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a novel silk protein scaffold and its preparation method, which overcomes the problem that the prior art cannot prepare low-crystallinity water-insoluble silk protein scaffolds

Method used

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  • Low-crystal fibroin bracket and preparation method thereof
  • Low-crystal fibroin bracket and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Soak about 10g raw silk in 0.5L 0.5% Na 2 CO 3 solution, stirred and boiled for 60 minutes, then removed and washed with deionized water. After repeating the above operation twice, the silk was dried at 60°C.

[0036] 5 g of the degummed silk after the above treatment was weighed and dissolved in 30 mL of LiBr solution with a concentration of 9.8 mol / L, stirred and dissolved at 60° C. for one hour. Then use a dialysis bag with a molecular weight cut off of 3500 to dialyze with deionized water for 3 days to obtain a silk protein aqueous solution with a concentration of 6%, and further adjust the concentration of the silk protein aqueous solution to 2%.

[0037] Take 10 mL of the above silk protein solution, add 5 mL of 0.5% ethylene glycol solution, stir evenly, pour it into a polyethylene mold, and then put the mold containing the mixed solution into -20 ° C for 6 hours to freeze. Then take out the frozen body, put it into deionized water to thaw for 1 hour, then rin...

Embodiment 2

[0040] Soak about 50g raw silk in 2L 0.05% Na 2 CO 3 solution, stirred and boiled for 60 minutes, then removed and washed with deionized water. After repeating the above operation twice, the silk was dried at 60°C.

[0041] 15 g of the degummed silk after the above treatment was weighed and dissolved in 100 mL of LiBr solution with a concentration of 9.8 mol / L, stirred and dissolved at 60° C. for one hour. Then use a dialysis bag with a molecular weight cut-off of 3500 to dialyze with deionized water for four days to obtain a silk protein aqueous solution with a concentration of 7%, and adjust the concentration of the silk protein aqueous solution to 4%.

[0042] Take 15 mL of the above silk protein solution, blend it with 15 mL of ethanol with a mass fraction of 0.1%, and then pour it into a polyethylene mold and freeze it at -20°C for 10 hours. After being frozen, it was taken out, thawed in the air at natural temperature for 2 hours, rinsed with deionized water for 3 hou...

Embodiment 3

[0045] Soak about 50g raw silk in 2L 0.05% Na 2 CO 3 solution, stirred and boiled for 60 minutes, then removed and washed with deionized water. After repeating the above operation twice, the silk was dried at 60°C.

[0046] 15 g of the degummed silk after the above treatment was weighed and dissolved in 100 mL of LiBr solution with a concentration of 9.8 mol / L, stirred and dissolved at 60° C. for one hour. Then use a dialysis bag with a molecular weight cut-off of 3500 to dialyze with deionized water for four days to obtain a silk protein aqueous solution with a concentration of 7%, and adjust the concentration of the silk protein aqueous solution to 5%.

[0047] Take 15 mL of the above silk protein solution, blend it with 30 mL of methanol with a mass fraction of 0.01%, and then pour it into a polyethylene mold and freeze it at -20°C for 12 hours. After being frozen, it was taken out, thawed in the air at natural temperature for 1 hour, rinsed with deionized water for 3 ho...

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Abstract

The invention relates to a preparation method of a low-crystal fibroin bracket. The preparation method comprises the following steps of uniformly co-mixing a fibroin aqueous solution with a low-concentration micromolecule alcohol aqueous solution to obtain a fibroin mixed solution, inducing fibroin molecules to generate twining and twisting through micromolecule alcohol, and besides, avoiding transformation of fibroin conformation through low concentration of the micromolecule alcohol, then freezing the fibroin mixed solution to obtain fibroin frozen bodies, and promoting occurrence of phase separation through a freezing icing process; then unfreezing the fibroin frozen bodies, and forming a three-dimensional porous bracket having a uniform structure through melting of ice nuclei; and finally preparing a dry-state low-crystal fibroin porous bracket easy to store, through a freeze-drying technology.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a low fibroin scaffold and a preparation method thereof. Background technique [0002] There are millions of patients with organ or tissue damage and functional loss due to diseases and accidents every year. In the United States alone, more than 8 million operations are needed to treat such patients every year, and the economic cost is more than 400 billion US dollars. With the development of modern medicine and surgical techniques, tissue or organ transplantation to repair functional loss has been widely accepted, however, it faces a huge donor gap. The formation of tissues or organs in vivo or in vitro by means of regenerative medicine provides a new treatment option for the repair of damaged functions. Among them, the selection and construction of tissue engineering scaffold materials become one of the keys to this treatment method. [0003] Silk protein is a natural polymer bioma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/50A61L27/56A61L27/58
CPCA61L27/227A61L27/50A61L27/56A61L27/58C08L89/00
Inventor 张锋路青青陆福男范志海秦建忠张鹏高洋陆史俊左保齐
Owner 上海丝波敦生物科技有限公司
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