Jatropha curcas extract insecticidal sustained-release gel and preparation method thereof

A slow-release gel and extract technology, applied in the field of biotechnology, to avoid the use of toxic and harmful organic solvents, improve the use time, and achieve the effects of large output

Inactive Publication Date: 2020-01-31
凉山德农生物能源股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are not many patents related to alkaloid extracts from Jatropha jatropha leaves, and there are no reports of patents that successfully use bio-based water-soluble macromolecules as alkaloid carriers and further prepare sustained-release pesticide gels

Method used

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  • Jatropha curcas extract insecticidal sustained-release gel and preparation method thereof
  • Jatropha curcas extract insecticidal sustained-release gel and preparation method thereof
  • Jatropha curcas extract insecticidal sustained-release gel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Such as figure 1 As shown, (1) add 20.5Kg of chitosan and 3Kg of acetic acid to 30.5Kg of water, stir vigorously with ultrasonic at 25°C for 1h, with ultrasonic power of 50W, then put it into a refrigerator at -50°C for 2h and take it out to thaw. Continue to freeze and thaw four times, centrifuge at 8000r / min for 30min to remove air bubbles, and let stand for 20min to obtain a clear solution.

[0031] (2) Dissolve the alkaloid extract mixture extracted from Jatropha Jatropha leaves in dichloromethane at 25°C, and rinse with a mixed solvent of petroleum ether / ethyl acetate (volume ratio 3:1) in a 400-mesh chromatography silica gel column. After washing for 12 hours, the eluent was rotary evaporated to obtain 3-methyloxazol-2-one. Add 25.4Kg of 3-methyloxazol-2-one into the above clear solution, stir at 12000r / min at high speed for 15min, and at the same time add 5Kg of calcium chloride dropwise, then stir at 5000r / min for 5 minutes. Let it stand for 5h to obtain a slo...

Embodiment 2

[0037] (1) Add 30.2Kg of sodium alginate and 1Kg of itaconic acid to 52.2Kg of water, stir vigorously for 3 hours at 20°C with ultrasonic power of 30W, put it in a refrigerator at -20°C for 4 hours, take it out and thaw it, and do the same Continue to freeze and thaw twice, centrifuge at 10,000r / min for 45min to remove air bubbles, and let stand for 30min to obtain a clear solution.

[0038] (2) Dissolve the alkaloid extract mixture extracted from Jatropha Jatropha leaves in dichloromethane at 20°C, and rinse with a mixed solvent of n-hexane / acetone (volume ratio 2:1) in a 500-mesh chromatography silica gel column for 24 hours , and then the eluent was rotary evaporated to obtain 6-hydroxypurine derivatives. Add 15.6Kg of 6-hydroxypurine derivatives to the above clear solution, stir at high speed for 30min at 10000r / min, add 1Kg of glutaraldehyde dropwise at the same time, stir at 7500r / min for 3 minutes, and let stand at 20°C for 3h Get slow release gel.

[0039] Figure 4...

Embodiment 3

[0044] (1) Add 25.6Kg of chitin and 2Kg of phytic acid to 40.3Kg of water, stir vigorously with ultrasonic at 30°C for 2h, with ultrasonic power of 40W, put it in a refrigerator at -80°C for 1h, take it out and thaw it out, and continue in the same way Freeze and thaw three times, centrifuge at 9000r / min for 60min to remove air bubbles, and let stand for 25min to obtain a clear solution.

[0045] (2) Dissolve the alkaloid extract mixture extracted from Jatropha Jatropha leaves in dichloromethane at 30°C, and rinse with a mixed solvent of acetone / ethanol (volume ratio 5:1) in a 400-mesh chromatography silica gel column 40h, and then the eluent was rotatably evaporated to obtain paromomycin. Add 29.1Kg of paromomycin to the above clarified solution, stir at 7500r / min at high speed for 20min, add 3Kg of genipin at the same time, stir at 6000r / min for 4 minutes, and stand at 30°C for 4h to obtain slow release gel.

[0046] Take a small amount of the above-mentioned slow-release ...

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Abstract

The invention provides jatropha curcas extract insecticidal sustained-release gel, and belongs to the technical field of biotechnology. The jatropha curcas extract insecticidal sustained-release gel is prepared from the following raw materials in percentage by weight: 15.6%-34.2% of jatropha curcas leaf alkaloid extract, 20.5%-30.2% of a bio-based macromolecular carrier, 30.5%-52.2% of water, 1%-5% of a cross-linking agent and 1%-3% of an auxiliary dispersing agent. The preparation method comprises the following steps: (1) adding the 20.5%-30.2% by weight of the bio-based macromolecular carrier and the 1%-3% of the auxiliary dispersing agent into the 30.5%-52.2% by weight of water; performing ultrasonic stirring at the temperature of between 20 and 30 DEG C, freezing in a refrigerator fora period of time, taking out, unfreezing, repeating the same method for 2 to 4 times, centrifuging at the speed of between 8000 and 10000 revolutions per minute for 30 to 60 minutes to remove bubbles,and standing for 20 to 30 minutes to obtain a clarified solution; and (2) adding the 15.6%-34.2% by weight of the jatropha curcas leaf alkaloid extract into the clarified solution at 20-30 DEG C, adding the 1%-5% by weight of the cross-linking agent while stirring, stirring for 3-5 minutes, and standing at 20-30 DEG C for 3-5 hours to obtain the slow-release gel.

Description

technical field [0001] The invention belongs to the technical field of biotechnology, and in particular relates to an insecticidal slow-release gel of Jatropha jatropha extract and a preparation method thereof. Background technique [0002] With the enhancement of people's awareness of environmental protection and concern for food safety, the negative effects of traditional chemical pesticides have become more and more prominent. The development of biological source pesticides has become an important trend to protect human health and lead the sustainable development of agriculture. Plants are rich in secondary metabolites, some of which have become research hotspots in recent years because of their certain effects in preventing and controlling diseases and insect pests. The types of plant secondary metabolites include alkaloids, phenols, terpenes, flavonoids, essential oils, photoactivated toxins, etc. Among them, alkaloids are the category with the largest content. They ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/18A01N43/80A01N43/90A01N43/16A01N45/02A01N25/10A01P7/04
CPCA01N25/10A01N43/16A01N43/80A01N43/90A01N45/02A01N65/18
Inventor 曾伟赵丽娟易龙飞宋永娇高元吉史英博张欣汝
Owner 凉山德农生物能源股份有限公司
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