A method for establishing an animal model of osteoporosis combined with Alzheimer's disease and its application
A technology for osteoporosis and establishment of methods, applied in animal husbandry and other directions, can solve the problems of unknown drug interactions, unsatisfactory efficacy, strong toxic and side effects, etc., and achieve the effects of easy application and promotion, short experimental period and low cost.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0029] Construction of an animal model of osteoporosis combined with Alzheimer's disease
[0030] 1. Establishment of postmenopausal osteoporosis model
[0031] Eight-week-old female Wistar rats, SPF grade, underwent bilateral ovariectomy after adaptive feeding for one week. The anesthetized rats were fixed in the prone position, and the skin was prepared on the backs of both sides. First, cut a small incision with surgical scissors from one side of the back and abdomen, cut the skin, abdominal muscle, and peritoneum in sequence, and then peel off the fat tissue to find the reddish-brown cauliflower-like ovary, which is ligated with surgical sutures on the uterine horn and removed. Then the abdominal muscles and skin were sutured sequentially, and penicillin powder was applied externally to the wound. The other ovary was removed in the same way. In the sham operation group, only the same volume of adipose tissue near the ovary was removed, and other operations were the same...
Embodiment 2
[0072] Intervention Validation in Animal Models of Osteoporosis and Alzheimer's Disease
[0073] 1. Experimental method
[0074] Establish the Wistar rat osteoporosis combined with Alzheimer's disease animal model according to the method described in Example 1, treat the classic drug estradiol valerate of osteoporosis in clinic, namely Estradiol Valerate, be called for short EV, treat Alzheimer's disease Donepezil hydrochloride, the classic drug of Donepezil HCL, abbreviated as DPZ, was administered to the animal model of the present invention respectively. Among them, EV at a dose of 0.6 mg / kg / d and DPZ at a dose of 0.8 mg / kg / d were intragastrically administered during the Alzheimer's disease modeling stage. After continuous administration for 8 weeks, various quantitative indicators of osteoporosis and Alzheimer's disease were detected with reference to Example 1 of the present invention.
[0075] 2. Experimental results
[0076]In the Morris water maze test, compared wit...
Embodiment 3
[0079] Discovery of dual-effect drugs based on echinacoside, verbascoside and their combination based on animal models of osteoporosis and Alzheimer's disease
[0080] 1. Experimental method
[0081] According to the method described in Example 1, an animal model of Wistar rat osteoporosis combined with Alzheimer's disease was established, and EV and DPZ were respectively administered to the animal model of the present invention according to the method described in Example 2, as two groups of positive control groups. Candidate drugs echinacoside (ECH group), verbascoside (ACT group) and the combination of two monomer drugs (P1 group) were administered to the animal model of osteoporosis combined with Alzheimer's disease of the present invention respectively. Among them, the dose of 90 mg / kg / d in the ECH group, the dose of 90 mg / kg / d in the ACT group, and the dose of 1:1 ratio of ECH and ACT in the P1 group were given by intragastric administration during the Alzheimer's diseas...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More