Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation and application of light-triggered erythrocyte membrane wrapped NO nano bionic donor material

A red blood cell membrane and nano-bionic technology, applied in the field of biomedical engineering materials, can solve the problems of NO nano-donor materials in the field of tumor treatment, difficult application of NO nano-donor materials, poor stability of NO donor materials, etc., to achieve inhibition Effects of tumor growth and distant metastasis, excellent cell specificity, and prolonged blood circulation time

Inactive Publication Date: 2020-02-07
JINAN UNIVERSITY
View PDF6 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, at present, NO nano-donor materials are difficult to be applied clinically. As a result, NO nano-donor materials are very difficult in the field of tumor treatment, and there are few research reports

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation and application of light-triggered erythrocyte membrane wrapped NO nano bionic donor material
  • Preparation and application of light-triggered erythrocyte membrane wrapped NO nano bionic donor material
  • Preparation and application of light-triggered erythrocyte membrane wrapped NO nano bionic donor material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Synthesis of polydopamine nanoparticles encapsulated by thiolated mesoporous silica (PDA@mSiO 2 )

[0058] a. Add tris(hydroxymethyl)aminomethane to a certain volume of deionized water at a room temperature of 15°C to form a certain concentration of Tris-HCl buffer solution, and then add a certain volume of isopropanol (IPA) to adjust the pH value of the mixed solution to 8.0, add dopamine hydrochloride under vigorous stirring. After continuous stirring for 2 days, the polydopamine nanoparticles were collected by centrifugation at 40,000 g for 10 min, and washed twice with deionized water and ethanol respectively to obtain polydopamine (PDA) nanoparticles. The molar ratio of Tris-HCl to dopamine hydrochloride is 1:0.1; the purity of the isopropanol is ACS, ≥99.5%; the volume ratio of the Tris-HCl buffer to isopropanol is 1:0.2; the Add 10 mg of tris(hydroxymethyl)aminomethane per 10 mL of deionized water.

[0059] b. Disperse the polydopamine nanoparticles obtained i...

Embodiment 2

[0061] Synthesis of polydopamine nanoparticles encapsulated by thiolated mesoporous silica (PDA@mSiO 2 )

[0062]c. Add tris(hydroxymethyl)aminomethane to a certain volume of deionized water at a room temperature of 25°C to form a certain concentration of Tris-HCl buffer solution, and then add a certain volume of isopropanol (IPA) to adjust the pH value of the mixed solution to 9.0, add dopamine hydrochloride under vigorous stirring. After continuous stirring for 4 days, the polydopamine nanoparticles were collected by centrifugation at 60000 g for 20 min, and washed three times with deionized water and ethanol respectively to obtain polydopamine (PDA) nanoparticles. The molar ratio of Tris-HCl to dopamine hydrochloride is 1:0.5; the purity of the isopropanol is ACS, ≥99.5%; the volume ratio of the Tris-HCl buffer to isopropanol is 1:0.5; the Add 15 mg of tris(hydroxymethyl)aminomethane per 10 mL of deionized water.

[0063] d. Disperse the polydopamine nanoparticles obtain...

Embodiment 3

[0065] Preparation of double-loaded polydopamine@mesoporous silica nanoparticles (PDA@mSiO 2 / SNO / DOX)

[0066] e. At room temperature, disperse the thiolated polydopamine@mesoporous silica nanoparticles and tert-butyl nitrite obtained in Example 1 in a mixed solution of 10 mL of methanol and toluene, and keep stirring for 24 hours under the protection of nitrogen in the dark. Then centrifuged at 9000rpm for 10min, washed twice with absolute ethanol, and dried at room temperature to finally obtain NO-loaded polydopamine@mesoporous silica nanoparticles (PDA@mSiO 2 / SNO); the mass ratio of the thiolated polydopamine@mesoporous silica nanoparticles to t-butyl nitrite is 1:1; the volume ratio of the two components in the mixed solution of methanol and toluene is 1:4 .

[0067] f. PDA@mSiO obtained by taking a certain mass of step e at room temperature 2 / SNO dispersed in absolute ethanol, then add a certain quality of doxorubicin hydrochloride (DOX), keep stirring in the dark f...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses preparation and an application of a light-triggered erythrocyte membrane wrapped NO nano bionic donor material. The method comprises the following steps: (1) synthesizing a sulfhydrylated mesoporous silica wrapped polydopamine nanoparticle, (2) preparing a dual-load polydopamine (PDA) @ mesoporous silica nanoparticle, (3) preparing a target marked erythrocyte membrane, and(4) preparing a target molecular marked erythrocyte membrane wrapped dual-load polydopamine @ mesoporous silica nanoparticle. The PDA@mSiO2 nanoparticle with good photothermal conversion performance is successfully integrated in an NO bionic nanoparticle structure; a cell membrane can be ablated to effectively release a loaded medicine under stimulation of near infrared light; at the same time, amore obvious killing effect can be caused on tumor cells; and therefore, a combined application of various treatment means is achieved.

Description

technical field [0001] The invention belongs to the field of biomedical engineering materials, and in particular relates to the preparation and application of a light-triggered red blood cell membrane-wrapped NO nanometer bionic donor material. Background technique [0002] Cancer is a major public health problem worldwide and it remains one of the leading causes of human death. Skin cancer is one of the most common cancers in the world, and malignant melanoma is the deadliest skin cancer with a high potential for recurrence and metastasis. This aggressive tumor, characterized by the largest mutation rate, is currently a difficult research point. Although chemotherapy is the main means of clinical cancer treatment, chemotherapy is often accompanied by serious side effects of chemotherapy drugs and the specificity of tumor treatment, which may lead to tumor recurrence and metastasis, affecting patients' satisfaction with prognosis. As we all know, nanomaterials have made gr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K41/00A61K31/704A61K33/00A61K9/51A61K47/46A61K47/52A61K47/59A61P35/00A61P17/00C08G73/06
CPCA61K9/5184A61K31/704A61K33/00A61K41/0052A61K41/0057A61K47/52A61K47/59A61P17/00A61P35/00C08G73/0672A61K2300/00
Inventor 张武李国巍马栋
Owner JINAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com