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A composite dressing that promotes wound healing and regeneration and loads traditional Chinese medicine exosomes and its preparation method

A wound healing and exosome technology, which is used in medical science, bandages, etc. to achieve the effects of rapid cross-linking, simple and convenient operation, and a wide range of sources.

Active Publication Date: 2021-06-15
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after loading traditional Chinese medicine exosomes and cytokines, it is still blank in the field of trauma therapy and angiogenesis.

Method used

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  • A composite dressing that promotes wound healing and regeneration and loads traditional Chinese medicine exosomes and its preparation method
  • A composite dressing that promotes wound healing and regeneration and loads traditional Chinese medicine exosomes and its preparation method
  • A composite dressing that promotes wound healing and regeneration and loads traditional Chinese medicine exosomes and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1: A composite dressing and preparation method for promoting trauma healing and regeneration and loading traditional Chinese medicine, including the following steps:

[0051] 1) The gelatin was reacted with methacrylic anhydride, followed by room temperature in a dialysis bag of 8K-14 kDa, then lyophilized, prepared a methacrylate gelatin;

[0052] 2) First put the girl, wash, juice, over 100 mesh sieve; then by differential centrifugation, after 1000g, 12000g centrifuge, take the supernatant to centrifuge at 100000g, then use precipitates PBS is resuspended. The density gradient solution was equipped with sucrose and then over 60 minutes at 200,000 g; and the outer body of different components was centrifuged at 50,000 to 50 minutes, and prepared as a Chinese medicine extracter;

[0053]3) Esterification of methacrylate was dissolved, and its concentration was 5%; then SDF-1 was added to 1 μg / ml of the concentration; and then the episode of the traditional Chines...

Embodiment 2

[0058] Example 2: A composite dressing and preparation method for promoting trauma healing and regeneration and loading traditional Chinese medicine, including the following steps:

[0059] 1) The gelatin was reacted with methacrylic anhydride, followed by room temperature in a dialysis bag of 8K-14 kDa, then lyophilized, prepared methacrylate gelatin;

[0060] 2) First put the salvia, wash, juice, over 200 mesh sieve; then pass the differential centrifugation method, then use 2000G, 10000g centrifuge, take the supernatant to centrifuge at 120,000 g, then use PBS to precipitate Resuspended. With a CSCl gradient solution, then over 60 minutes at 100,000 g; then taking the outer body of different components, centrifugation at 100,000 g for 60 minutes, and prepared as a Chinese medicine extracter;

[0061] 3) Esterification of methacrylate was dissolved with PBS, allowing its concentration to 10%; then add SDF-1 to reach 0.2 μg / ml; then add traditional Chinese medicine, so that it ...

Embodiment 3

[0067] Example 3: A composite dressing and preparation method for promoting trauma healing and regeneration and loading traditional Chinese medicine, including the following steps:

[0068] 1) The gelatin was reacted with methacrylic anhydride, and then in the dialysis bag of 8k-14 kDa was dialyzed for 2 weeks, then lyophilized, prepared metylhalic acid esterification gelatin;

[0069] 2) First, we will wash, wash, juice, over 150 mesh sieve; then pass the differential centrifugation method, then use 3000g, 5000g centrifuge, take the supernatant to centrifuge at 100,000 g, then precipitate Rehabilitate with ultrapure water. Using a sucrose gradient solution and then over 100,000 g of 60 minutes; then taking the outer body of different components, centrifugation at 10,000 g of 60 minutes, and prepared a Chinese medicine extracter;

[0070] 3) Ultra-pure water is dissolved by ultrapure water by ultrapure water, and then SDF-1 is added to increase its concentration to 0.5 μg / mL; th...

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Abstract

The invention provides a composite dressing that promotes wound healing and regeneration and loads traditional Chinese medicine exosomes and a preparation method thereof, comprising the following steps: preparing methacrylated gelatin by reacting gelatin with methacrylic anhydride; Prepare traditional Chinese medicine exosomes; compound the prepared exosomes with methacrylated gelatin, and add SDF‑1 and a photosensitizer to prepare a pre-gel system; form a composite dressing by crosslinking with ultraviolet light. The dressing prepared in the present invention is a new type of photosensitive dressing compounded with traditional Chinese medicine exosomes. After forming a pre-gel system, it can be cross-linked by ultraviolet light to form a composite dressing in vitro, and can also be cross-linked in situ at the wound. The cross-linking is fast, simple and convenient, and can effectively protect the wound area, induce the migration of bone marrow mesenchymal stem cells, cooperate with traditional Chinese medicine exosomes to promote wound healing and regeneration, and effectively solve the current problems in the process of wound healing and regeneration. Therefore, the composite dressing of the present invention has wide application value in wound healing and regeneration.

Description

Technical field [0001] The present invention relates to a composite dressing and preparation method for promoting wound healing and regeneration and loading of Chinese medicine extractemed. Background technique [0002] The skin is the first line of defense of the human body, and it is also a largest organ, has a variety of functions such as feelings and barriers. Skin can form an effective barrier between organisms and environments to prevent invasion of pathogens, resist chemistry and physical invasion. However, in daily life, the skin is often influenced by a variety of external factors, causing trauma, and the loss of large area often brings serious consequences, leading to serious disability and even death, bringing heavy stress and burden to individuals and society. [0003] The healing of wounds is a complex and dynamic process, including a variety of tissue and cells, and the like to participate in regulation. The wound repair process is not controlled, often leads to ser...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L26/00
CPCA61L26/0038A61L26/0047A61L26/0066A61L26/008A61L2300/252A61L2300/30A61L2300/412A61L2300/45
Inventor 彭丽华许学寒
Owner ZHEJIANG UNIV
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