A composite dressing that promotes wound healing and regeneration and loads traditional Chinese medicine exosomes and its preparation method
A wound healing and exosome technology, which is used in medical science, bandages, etc. to achieve the effects of rapid cross-linking, simple and convenient operation, and a wide range of sources.
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Embodiment 1
[0050] Example 1: A composite dressing and preparation method for promoting trauma healing and regeneration and loading traditional Chinese medicine, including the following steps:
[0051] 1) The gelatin was reacted with methacrylic anhydride, followed by room temperature in a dialysis bag of 8K-14 kDa, then lyophilized, prepared a methacrylate gelatin;
[0052] 2) First put the girl, wash, juice, over 100 mesh sieve; then by differential centrifugation, after 1000g, 12000g centrifuge, take the supernatant to centrifuge at 100000g, then use precipitates PBS is resuspended. The density gradient solution was equipped with sucrose and then over 60 minutes at 200,000 g; and the outer body of different components was centrifuged at 50,000 to 50 minutes, and prepared as a Chinese medicine extracter;
[0053]3) Esterification of methacrylate was dissolved, and its concentration was 5%; then SDF-1 was added to 1 μg / ml of the concentration; and then the episode of the traditional Chines...
Embodiment 2
[0058] Example 2: A composite dressing and preparation method for promoting trauma healing and regeneration and loading traditional Chinese medicine, including the following steps:
[0059] 1) The gelatin was reacted with methacrylic anhydride, followed by room temperature in a dialysis bag of 8K-14 kDa, then lyophilized, prepared methacrylate gelatin;
[0060] 2) First put the salvia, wash, juice, over 200 mesh sieve; then pass the differential centrifugation method, then use 2000G, 10000g centrifuge, take the supernatant to centrifuge at 120,000 g, then use PBS to precipitate Resuspended. With a CSCl gradient solution, then over 60 minutes at 100,000 g; then taking the outer body of different components, centrifugation at 100,000 g for 60 minutes, and prepared as a Chinese medicine extracter;
[0061] 3) Esterification of methacrylate was dissolved with PBS, allowing its concentration to 10%; then add SDF-1 to reach 0.2 μg / ml; then add traditional Chinese medicine, so that it ...
Embodiment 3
[0067] Example 3: A composite dressing and preparation method for promoting trauma healing and regeneration and loading traditional Chinese medicine, including the following steps:
[0068] 1) The gelatin was reacted with methacrylic anhydride, and then in the dialysis bag of 8k-14 kDa was dialyzed for 2 weeks, then lyophilized, prepared metylhalic acid esterification gelatin;
[0069] 2) First, we will wash, wash, juice, over 150 mesh sieve; then pass the differential centrifugation method, then use 3000g, 5000g centrifuge, take the supernatant to centrifuge at 100,000 g, then precipitate Rehabilitate with ultrapure water. Using a sucrose gradient solution and then over 100,000 g of 60 minutes; then taking the outer body of different components, centrifugation at 10,000 g of 60 minutes, and prepared a Chinese medicine extracter;
[0070] 3) Ultra-pure water is dissolved by ultrapure water by ultrapure water, and then SDF-1 is added to increase its concentration to 0.5 μg / mL; th...
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