A kind of homogeneous anode photoelectrochemical detection method of aflatoxin based on bismuth tungstate

A kind of aflatoxin, photoelectrochemical technology, applied in the field of analysis and detection, analysis and detection technology, can solve the problems of complex operation steps, decreased recognition efficiency, low sensitivity, etc., and achieve the effect of high sensitivity, simple operation and low cost

Active Publication Date: 2022-07-26
JIANGNAN UNIV
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Most of the currently reported photoelectrochemical immunosensors or aptasensors for aflatoxin detection require high-cost antibodies or labeled nucleic acid probes, which have high cost, complicated operation steps, and low sensitivity (because of the recognition of biomolecules immobilized on the surface Efficiency tends to drop), etc.

Method used

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  • A kind of homogeneous anode photoelectrochemical detection method of aflatoxin based on bismuth tungstate
  • A kind of homogeneous anode photoelectrochemical detection method of aflatoxin based on bismuth tungstate
  • A kind of homogeneous anode photoelectrochemical detection method of aflatoxin based on bismuth tungstate

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Experimental program
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Effect test

Embodiment 1

[0027] a. Bi 2 WO 6 Preparation: Weigh 0.3g of bismuth nitrate pentahydrate and dissolve it in 20mL of dilute nitric acid with pH=1, stir magnetically until it dissolves, and count it as solution A; weigh 0.17g of sodium tungstate dihydrate and dissolve it in 20mL of deionized water, Stir magnetically until dissolved, and count as solution B; add solution B dropwise to solution A, stir for 20 minutes, transfer into a 50 mL autoclave, and heat at 180 ° C for 12 hours; after natural cooling to room temperature, anhydrous ethanol and distilled water are alternated Wash and dry overnight at 60°C in a vacuum oven;

[0028] b. Preparation of Bi 2 WO 6 / ITO electrode: Weigh the pre-prepared Bi2 WO 6 The powder was added to deionized water and sonicated to obtain a suspension with a concentration of 1 mg / mL; 30 μL of Bi was dripped on the surface of the pre-cleaned ITO electrode. 2 WO 6 The suspension was dried at 40°C for 2 hours for use;

[0029] c. Biometric recognition and ...

Embodiment 2

[0032] a. Bi 2 WO 6 Preparation: Weigh 0.3g of bismuth nitrate pentahydrate and dissolve it in 20mL of dilute nitric acid with pH=1, stir magnetically until dissolved, and count as solution A; weigh 1.49g of ammonium tungstate and dissolve it in 20mL of deionized water, stir magnetically Until dissolved, it was counted as solution B; solution B was added dropwise to solution A, stirred for 20 minutes, transferred to a 50 mL autoclave, heated at 160 ° C for 16 hours; after naturally cooled to room temperature, washed alternately with absolute ethanol and distilled water and Dry overnight at 40°C in a vacuum oven;

[0033] b. Preparation of Bi 2 WO 6 / ITO electrode: Weigh the pre-prepared Bi 2 WO 6 The powder was added to deionized water and sonicated to obtain a suspension with a concentration of 1 mg / mL; 30 μL of Bi was dripped on the surface of the pre-cleaned ITO electrode. 2 WO 6 The suspension was dried at 40°C for 2 hours for use;

[0034] c. Biometric recognition...

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Abstract

The invention belongs to the field of analysis and detection, and relates to a bismuth tungstate-based homogeneous anode photoelectrochemical detection method for aflatoxin. The ITO electrode modified by bismuth tungstate was used as the photoanode, and the signal molecule (methylene blue or thioflavin T) could act as an electron donor to increase the photocurrent of the anode. In a homogeneous solution, a signal "enhanced" was constructed by combining the rolling circle amplification (RCA) reaction mediated by the recognition reaction of aflatoxin and aptamer and the specific embedding of the signal molecule into the G-quadruplex. detection platform. The invention has high detection sensitivity for aflatoxin, the linear range is 0.01-10000pg / mL, and the detection limit is as low as 2.6fg / mL. Compared with the traditional method, the method proposed in the present invention has low cost, simple operation (no need for labeling and electrode immobilization of biomolecules), small amount of reagents and strong practicability, and is expected to be one of the efficient methods for detecting aflatoxin.

Description

technical field [0001] The invention relates to an analysis and detection technology, and belongs to the technical field of analysis and detection. Background technique [0002] Describe the state of the art and problems with the closest prior art to the present invention. [0003] Aflatoxins are mainly produced by Aspergillus flavus and Aspergillus parasiticus [Reverberi M, Ricelli A, ZjalicS, Fabbri A A, Fanelli C. Appl. and teratogenic mycotoxins [Rawal S, Kim J E, Coulombe Jr R. Res. Vet. Sci. 2010, 89:325-331.]. Aflatoxins are classified as Group 1 human carcinogens by the International Agency for Research on Cancer (IARC). Among the natural pollution of grain, oil and food, aflatoxin is the most common, and the most toxic and carcinogenic. Among them, peanut, peanut oil and corn are the most serious, and its toxicity is 10 times that of potassium cyanide. Food manufacturers spend between $500 and $1.5 billion annually to manage mycotoxins [RobensJ, Cardwell K. Journ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6804G01N27/26
CPCC12Q1/6804G01N27/26C12Q2531/125C12Q2565/607
Inventor 王光丽刘田利孙冬雪顾萌萌
Owner JIANGNAN UNIV
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