Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Traditional Chinese medicine composition for treating diabetic nephropathy and application thereof

A composition and technology of traditional Chinese medicine, applied in the field of traditional Chinese medicine composition for the treatment of diabetic nephropathy, can solve problems such as unreported treatment, achieve good fasting blood sugar, reduce fasting blood sugar, and have good safety effects

Inactive Publication Date: 2020-02-14
雷允上药业集团有限公司
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Not reported for the treatment of diabetes or diabetic nephropathy

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Traditional Chinese medicine composition for treating diabetic nephropathy and application thereof
  • Traditional Chinese medicine composition for treating diabetic nephropathy and application thereof
  • Traditional Chinese medicine composition for treating diabetic nephropathy and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A traditional Chinese medicine composition for treating diabetic nephropathy, which is prepared by the following method:

[0026] (1) Get Radix Notoginseng 153g, Ziheche 38g and Leech 38g by weight and pulverize into fine powder, cross 100 mesh sieves, sterilize, and set aside;

[0027] (2) Take astragalus 680g, medlar 170g, yam 255g, coix seed 170g, scrophulariaceae 170g, northern sand ginseng 170g, black lotus 170g, eucommia 340g, motherwort 510g, cicada slough 51g, plantain 170g and achyranthes bidentata 170g, decoct twice with water, 1 hour each time, combine the decoction, filter, and concentrate the filtrate under reduced pressure to a clear paste with a relative density of 1.16-1.20 at 60°C, add ethanol to make the alcohol content reach 63%, stir well, Stand still for 24 hours, take the supernatant to recover ethanol under reduced pressure and concentrate to a clear paste with a relative honeyness of 1.36-1.39 at 60°C; add step (1) Sanqi, Ziheche and leech fine p...

Embodiment 2

[0028] Example 2 Experiments on Lowering Blood Sugar, Lowering Blood Fat and Protecting Kidney Damage

[0029] Get 14-16 week-old SD rats, body weight (220±20) g, male barrier environment rats (SPF grade), and feed them adaptively for 5 days. During the experiment, 10 rats in the blank group were fed with conventional feed all the time, and the remaining rats were Feed high-sugar, high-fat and 0.5% glucose water all the time. After 3 weeks, inject STZ intraperitoneally at 30 mg / kg for 3 consecutive days. Fasting blood glucose was measured by venous blood sampling, and the modeling was successful when the blood glucose value was higher than 13.1mmol / L. The diabetic rats successfully modeled were randomly divided into 5 groups according to the fasting blood glucose level, 10 in each group, respectively the model group and the metformin (positive control drug) group (13.5mg / kg·d) administered orally for 15 consecutive days. Example 1 The prepared traditional Chinese medicine com...

Embodiment 3

[0042] Example 3 Functional Experiments for Improving Renal Tubular Fibrosis

[0043] 1. Experimental cells

[0044] Renal tubular epithelial cell line HK-2, cultured in DMEM high-glucose medium containing 10% fetal bovine serum + 100 U / mL penicillin + 100 μg / mL streptomycin, placed at 37°C, 5% CO 2 in the incubator.

[0045] 2. Drugs

[0046] Positive drug DPI (NADPH oxidase inhibitor) was purchased from Calbiochem Corp. (La Jolla, CA).

[0047] 3. Grouping and processing of cells

[0048] Cells were plated in 96-well plates, pretreated with DMEM high-glucose medium, 37°C, 5% CO 2 Incubate for 6 hours to make it in a synchronized state. Change the medium every 24h. The experiment was divided into: a blank control group, that is, without adding intervention factors); a high-sugar treatment group; and the administration groups were DPI (10 μM) and the capsule treatment group of the traditional Chinese medicine composition of Example 1 (50 μM).

[0049] 4. Experimental an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of a traditional Chinese medicine composition in preparation of drugs for treating diabetes and diabetic nephropathy. The traditional Chinese medicine composition is prepared from radix astragali, fructus lycii, rhizoma dioscoreae, semen coicis, radix scrophularise, radix glehniae, herba ecliptae, dried human placenta, cortex eucommiae, radix notoginseng, herba leonuri, leeches, cicada ecdysis, semen plantaginis and radix achyranthis bidentatae. According to the application, proven by experimental researches, the traditional Chinese medicine composition has a very good effect of reducing fasting blood-glucose of rats suffering from the diabetic nephropathy, and the content of blood serum creatinine, urea nitrogen, kidney index and urinary protein in the rats suffering from the diabetic nephropathy can be remarkably lowered. Meanwhile, the traditional Chinese medicine composition has a very good effect of preventing renal tubular epithelial cells from fibrosis. Novel pharmaceutical preparations for clinical indications can be developed.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy, and in particular relates to a traditional Chinese medicine composition for treating diabetic nephropathy and its application. Background technique [0002] Diabetes is a common and frequently-occurring disease worldwide, and has become the third largest disease after tumors and cardiovascular diseases. Clinically, diabetes is often combined with other diseases, such as diabetes with nephropathy and diabetes with hyperlipidemia. Diabetes combined with kidney disease is caused by yin deficiency, dryness and heat, depletion of kidney qi, and loss of essence; damage, seriously affecting people's quality of life. [0003] At present, there are many drugs for the treatment of diabetes. Although the western medicines used clinically have certain curative effects, most of them have relatively large adverse reactions, such as rebound after drug withdrawal, liver and kidney damage, r...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/8994A61P3/10A61K35/64A61K35/62A61K35/50
CPCA61K35/50A61K35/62A61K35/64A61K36/21A61K36/23A61K36/258A61K36/28A61K36/46A61K36/481A61K36/533A61K36/68A61K36/808A61K36/815A61K36/8945A61K36/8994A61K2236/331A61K2236/51A61K2236/55A61P3/10A61K2300/00
Inventor 许鑫王恒斌潘耀宗李全翟云良罗年翠张邦国张丹丹戴晶晶
Owner 雷允上药业集团有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com