Dry sheet reagent used for quantitative detection of urea nitrogen

Abstract

The invention provides a dry sheet reagent used for the quantitative detection of urea nitrogen. The reagent comprises a shell, wherein a diffusion layer, a reagent layer, a resin layer and a base material are laminated in sequence in the shell; the center position of the upper surface of the shell is provided with a sample dripping hole; the center position of the bottom of the shell is providedwith a lightproof hole; the diffusion layer, the reagent layer and the re sin layer are hierarchically laminated on the base material in sequence from top to bottom through a coating technology; the diffusion layer mainly consists of cellulose acetate, titanium dioxide, surfactant, magnesium chloride, glycerol and polyethylene glycol; and the reagent layer mainly comprises urase, phenol red sodiumsalt, phosphoric acid buffer solutions, polyacrylamide, polyvinylpyrrolidone, gelatin and enzyme protectant EDTA (Ethylene Diamine Tetraacetic Acid). Through the reagent disclosed by the invention,the problems in the prior art that a wet chemical detection reagent is unstable and a test result has a big error can be solved.

Description

technical field [0001] The invention relates to a urea nitrogen detection reagent, in particular to a dry sheet reagent for quantitative detection of urea nitrogen. Background technique [0002] The so-called "dry reagent" is a relative comparison of the traditional "wet chemistry". It uses the liquid in the sample to be tested as the reaction medium, and the analyte directly reacts with the reagent immobilized on the carrier. The biggest difference between it and traditional wet chemistry is that the medium involved in the chemical reaction is different. With the development of enzyme separation, purification, storage and other technologies in biochemistry, the advancement of sensors, photometers, and electrode technologies, and the popularization of computers, dry chemistry has also developed rapidly. [0003] Compared with wet chemistry, dry chemistry has its unique advantages. As far as reagents are concerned, dry chemical reagents do not need to do pre-test preparation...

AI Technical Summary

Problems solved by technology

[0004] The invention provides a dry sheet reagent for quantitative detection of urea nitrogen to solve the problems in the prior art that the wet chemical detection reagent is unstable and the error of the test result is large

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