SNP marker for distinguishing ability of litopenaeus vannamei against vibrio harveyi infection and detection method thereof and application of SNP marker

An anti-Vibrio harveta and vanabine-resistant technology is applied in the SNP marker for distinguishing the anti-Vibrio harvetii infection ability of Litopenaeus vannamei and its detection and application fields, which can solve the problems of cumbersome operation, high cost and the like, and achieve low cost effect
CN110791571AActive Publication Date: 2020-02-14SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Publication Date
2020-02-14

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Abstract

The present invention discloses a SNP marker for distinguishing ability of litopenaeus vannamei against vibrio harveyi infection and a detection method thereof and an application of the SNP marker. The SNP marker is located at the 1191th site of a Lyz-I2 gene sequence of litopenaeus vannamei, with mutation type comprising G homozygote, heterozygote of G and three other nucleotides, and homozygoteor heterozygote of three other nucleotides except for G. The invention provides a SNP site for distinguishing ability of litopenaeus vannamei against vibrio harveyi infection, and develops a tetra-primer for amplifying genotype of the SNP site and the detection method for detecting the SNP genotype. The method can simply, rapidly and accurately detects polymorphism forms of the site at low cost, thereby facilitating to rapidly screen individual litopenaeus vannamei and breeding group with high ability against vibrio harveyi, and to maintain antibacterial varieties of litopenaeus vannamei against vibrio harveyi.
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Description

technical field

[0001] The invention belongs to the field of aquaculture biotechnology, more specifically, relates to the detection of gene single nucleotide polymorphism (SNP), in particular to a kind of i-2 type lysozyme gene of Litopenaeus vannamei, one of which causes different antibacterial ability to lyse bacteria The SNP molecular marker produced by the enzyme protein, and the detection method of the SNP marker genotype detected by the PCR detection method of the four-primer amplification hindered mutation system, and the antibacterial broodstock individual identification, antibacterial prawn population selection and antibacterial prawn strain maintenance. application. Background technique

[0002] Litopenaeus vannamei, commonly known as Litopenaeus vannamei, is the first cultured prawn species in the world. In my country, the annual output of Litopenaeus vannamei has reached 1.67 million tons, accounting for 83% of the total output of cultured prawns in the country....

Claims

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