Electrochemical detection method for rapidly detecting L-Cystine content in acid solution

An acid solution, rapid determination technology, applied in the field of electrochemical detection, can solve the problems of complex operation steps, high detection cost, long detection time, etc., and achieve the effects of simple production process, high sensitivity and fast detection speed.

Inactive Publication Date: 2020-02-21
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be directly used to detect the concentration of L-cystine in the electrolyte in the process of electrochemically reducing L-cystine to prepare L-cysteine, without the need for sample pretreatment, which solves the existing operation steps in the prior art Complexity, long detection time, high detection cost, etc.

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  • Electrochemical detection method for rapidly detecting L-Cystine content in acid solution
  • Electrochemical detection method for rapidly detecting L-Cystine content in acid solution
  • Electrochemical detection method for rapidly detecting L-Cystine content in acid solution

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Effect test

Embodiment 1

[0046] The pretreatment of the silver electrode of the present invention, the silver electrode of the present invention needs to be polished, polished, cleaned and other pretreatment processes, specifically comprising the following steps:

[0047] 1. Select a silver disc electrode with a diameter of 2 mm, and polish the electrode with 0.1 μm alumina powder.

[0048] 2. Physically polish the polished electrode with 800-mesh sandpaper.

[0049] 3. The polished silver electrode was ultrasonically cleaned with acetone and deionized water for 1 min respectively to obtain a pretreated silver electrode.

[0050] 4. Immerse the electrode in ethanol solution for later use.

Embodiment 2

[0052] Electrochemical detection method of L-cystine in acidic solution.

[0053] Blank control solution: add concentrated hydrochloric acid (mass fraction 37%) to water to prepare an acidic aqueous solution containing hydrochloric acid concentration between 0.5mol / L;

[0054] Standard solution: take 25mL of the above blank control solution, add 0.3mmol standard substance L-cystine to it, dissolve it by ultrasonic oscillation, dilute to 50mL with the above blank control solution, and prepare L-cystine with a concentration of 6.0mmol / L acid standard solution;

[0055] Inject the L-cystine standard solution with a concentration of 6.0mmol / L into the electrolytic cell, insert the working electrode, counter electrode, and reference electrode into the electrolytic cell, and immerse the electrode area below the liquid surface of the solution. Connect the working electrode, counter electrode, and reference electrode to the electrochemical workstation. The cyclic voltammetry curve o...

Embodiment 3

[0057] Determination of the L-cystine concentration in the sample during the preparation of L-cysteine ​​by electroreduction of L-cystine in acid solution.

[0058] 1. Solution preparation

[0059] Blank control solution: add concentrated hydrochloric acid (mass fraction 37%) to water to prepare an acidic aqueous solution containing hydrochloric acid concentration between 0.5mol / L;

[0060] standard solution:

[0061] Take 25mL of the above blank control solution, add 0.005mmol standard L-cystine to it, dissolve it by ultrasonic oscillation, and dilute to 50mL with the above blank control solution to prepare a standard solution of L-cystine with a concentration of 0.1mmol / L ;

[0062] Take 25mL of the above blank control solution, add 0.01mmol standard substance L-cystine to it, dissolve it by ultrasonic oscillation, dilute to 50mL with the above blank control solution, and prepare a standard solution of L-cystine with a concentration of 0.2mmol / L ;

[0063] Take 25mL of t...

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Abstract

The invention discloses an electrochemical detection method for rapidly detecting L-Cystine content in acid solution, and relates to the field of electrochemical detection. The unmodified silver electrode is used as a working electrode; the platinum electrode is used as a counter electrode; the Ag / AgC1 electrode is used as a reference electrode; and, according to the linear relation between the electrochemical response signal and the concentration of the to-be-detected object, the quantitative detection of the L-Cystine is performed conveniently, rapidly and precisely by using the cyclic voltammetry or the differential pulse voltammetry. After verification, the electrochemical detection method provided by the invention can rapidly, conveniently and precisely online detect the reaction process for electrochemically reducing the L-Cystine to composite the L-Cysteine by detecting the concentration of L-Cystine. The method does not depend on the large-scale instrument and equipment; the adopted unmodified silver electrode is simple in preparation and low in cost; the detection method is sensitive, simple, convenient and rapid for detecting the L-Cystine, can be used for detecting the L-Cystine content in acid solution, and is hopeful to be popularized in online monitoring the reaction process for electrochemically reducing the L-Cystine to composite the L-Cysteine.

Description

technical field [0001] The invention belongs to the technical field of electrochemical detection, in particular to an electrochemical detection method for quickly measuring the content of L-cystine in acidic solution. Background technique [0002] L-cystine (L-Cystine) refers to the left-handed form of cystine, a common sulfur-containing amino acid, with a relative molecular mass of 240.3 and a molecular formula of C 6 h 12 N 2 o 4 S 2 . L-cystine (the L-form of cystine) is a covalently dimeric non-essential amino acid formed by sulfur dimerization of two cysteines. There is a small amount in protein, and it is mostly contained in the keratin of hair, fingers and claws. L-cystine is found in many foods, including eggs, meat, dairy products, whole grains, and skin and hair. L-cystine is odorless, tasteless, white hexagonal plate-shaped crystals or crystalline powder. Soluble in dilute acid and alkaline solution, very slightly soluble in water, insoluble in ethanol, et...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/48G01N27/416
CPCG01N27/30G01N27/4163G01N27/48
Inventor 马红星陈松王鸿钰朱俊燕强华陈亮
Owner YANCHENG INST OF TECH
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