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Preparation of composite fluorescent nanoprobe and its detection method for hydrogen peroxide

A nano-probe and composite fluorescence technology, applied in the field of nano-probes, can solve the problems of detection environment interference, long reaction detection time, low selection specificity, etc., and achieve the later purification process easier, good detection selectivity, and high efficiency The effect of detecting the target

Active Publication Date: 2022-05-10
GUANGXI TEACHERS EDUCATION UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to the GB / T23499-2009 national standard, there are iodometric method and titanium salt colorimetric method about the methods for measuring the residual amount of hydrogen peroxide. Defects such as long detection time, inconvenient use for trace detection, slow response, and susceptibility to interference from the detection environment

Method used

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  • Preparation of composite fluorescent nanoprobe and its detection method for hydrogen peroxide
  • Preparation of composite fluorescent nanoprobe and its detection method for hydrogen peroxide
  • Preparation of composite fluorescent nanoprobe and its detection method for hydrogen peroxide

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Embodiment 1: the synthesis of CdSe@ZnS quantum dots

[0044] CdCl 2 2H 2 Dissolve O (38.4 mg) and NAC (27.5 mg) in 80 mL of ultrapure water, mix and stir evenly, adjust the pH of the solution to 9 with NaOH, add 170 μL of NaHSe solution, stir at room temperature for 2 h, and transfer the reaction solution to 100 °C Heated for 6 h, then added 3 mL of Zn(OAc) 2 2H 2 O and 3 mL of Na 2 S·9H 2 O solution to obtain CdSe@ZnS quantum dots.

[0045] Wherein, the concentration of NaOH is 1mol / L, NaHSe solution is made of Se powder (39.5mg) and NaBH 4 (40mg) was reacted in 2mL ultrapure water, Zn(OAc) 2 2H 2 O and Na 2 The concentrations of S were 11.5 mg / mL and 12 mg / mL, respectively.

[0046] The perspective electron microscope picture is as follows figure 1 As shown, the as-prepared CdSe@ZnS quantum dots show good dispersion and are spherical in shape, and the corresponding Gaussian fitting results of the histograms obtained from the TEM images of CdSe@ZnS quantum do...

Embodiment 2

[0047] Embodiment 2: Synthetic AgNCs

[0048] Weigh 52.6 mg of DL-lipoic acid and dissolve it in 20 mL of ultrapure water, add 400 μL of NaOH solution (1mol / L), stir until completely dissolved, then add 2 mL of NaBH 4 (0.24mg / mL) solution and 0.5mL of AgNO 3 (25mM) solution to prepare nano-silver clusters.

[0049] The perspective electron microscope picture is as follows figure 2 As shown, the synthesized nano-silver clusters are spherical, and the dispersion is good, and its particle size is 1.7 ± 0.5nm.

Embodiment 3

[0050] Example 3: Construction of CdSe@ZnS / AgNCs composite fluorescent nanoprobe

[0051] 20 μL of CdSe@ZnS quantum dot solution (3.2 mg / mL) and 20 μL of AgNCs solution (1.5 mg / mL) were diluted to 2 mL with Tris-HCl (pH=7.4) solution and incubated together for 10 min to prepare CdSe@ZnS ZnS / AgNCs composite fluorescent nanoprobes.

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Abstract

The invention discloses a method for preparing a CdSe@ZnS / AgNCs composite fluorescent nanoprobe, which comprises: mixing CdSe@ZnS quantum dots and nano-silver clusters under a certain pH condition to prepare a CdSe@ZnS / AgNCs composite fluorescent nanoprobe Needles; wherein, after mixing, the concentration of the CdSe@ZnS quantum dots is 0.032mg / mL, and the concentration of the nano-silver clusters is 0.015mg / mL. The present invention utilizes CdSe@ZnS quantum dots and nano-silver clusters to directly mix and construct a CdSe@ZnS / AgNCs composite fluorescent nanoprobe, which has the advantage of a simple synthesis method. The constructed CdSe@ZnS / AgNCs composite fluorescent nanoprobe oxidizes and decomposes silver clusters through hydrogen peroxide to release silver ions, which have a quenching effect on the fluorescence of CdSe@ZnS quantum dots, achieving the efficient detection of hydrogen peroxide. , so as to solve the technical problems of long detection reaction time, slow response and insufficient detection limit existing in traditional technology.

Description

technical field [0001] The invention relates to the technical field of nanoprobes, more specifically, the invention relates to the preparation of a composite fluorescent nanoprobe and its detection method for hydrogen peroxide. Background technique [0002] In recent decades, food safety issues have attracted people's attention more and more, and food safety issues have also been put on the agenda. The rich nutrients in food provide a good living environment for the growth of microorganisms. Hydrogen peroxide has an efficient fresh-keeping function on food, so people have developed food additive hydrogen peroxide in order to pursue the freshness of food. Hydrogen peroxide can inhibit the growth of microorganisms in food, so as to achieve the purpose of food preservation for a long time, but the residual hydrogen peroxide in food will seriously endanger human health. As one of the important members of active oxygen, hydrogen peroxide can cause DNA damage and aging of the bo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/02C09K11/88B82Y30/00G01N21/64
CPCC09K11/883C09K11/02B82Y30/00G01N21/643G01N2021/6432
Inventor 周志强杨立云黄凌刘义
Owner GUANGXI TEACHERS EDUCATION UNIV
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