A kind of nanometer medicine and its preparation method and application

A technology of nano-drugs and chemotherapeutic drugs, applied in the direction of nano-drugs, drug combinations, nanotechnology, etc., can solve the problems of tumors that cannot be loaded with drugs and photothermal agents, poor biocompatibility of gold nanorods, and inability to solve cell autophagy, etc. The preparation method is simple and easy, the tumor volume is suppressed, and the effect of reducing the number is achieved

Active Publication Date: 2021-12-31
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the gold nanorods coated with the material have poor biocompatibility and cytotoxicity, which may cause inflammatory reactions and cannot solve the problem of cell autophagy caused by the photothermal effect.
[0005] CN107309437A discloses a gold nanostar / calcium phosphate nanoparticle, the photothermal conversion efficiency of the gold nanostar / calcium phosphate nanoparticle is high, and the thermal imaging of the tumor site can be realized while the drug is delivered, and the gold nanostar / calcium phosphate nanoparticle Particles are mainly used for thermal imaging of tumor sites, and cannot be loaded with drugs and photothermal agents for tumor treatment

Method used

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  • A kind of nanometer medicine and its preparation method and application
  • A kind of nanometer medicine and its preparation method and application
  • A kind of nanometer medicine and its preparation method and application

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Experimental program
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Embodiment 1

[0062] The preparation of embodiment 1 nano drug

[0063] (1) Dissolve 0.1 mL of docetaxel with a concentration of 1 μg / mL in methanol, add 1 mL of sodium polyphosphate solution with a concentration of 20 mg / mL to obtain the first mixed solution; Calcium chloride solution was added to the first mixed solution to obtain the second mixed solution, which was centrifuged after 30 minutes of magnetic stirring at room temperature, and the precipitate was ultrasonically reconstituted to obtain the first dispersion, which contained docetaxel-encapsulated Calcium phosphate matrix;

[0064] (2) Add 10 mL of dopamine with a concentration of 1 mg / mL, adjust the pH to 8.5, centrifuge after 15 hours of magnetic stirring at room temperature, redissolve the precipitate and centrifuge, wash 3 times and redissolve the precipitate ultrasonically to obtain the second dispersion liquid. A calcium phosphate matrix containing surface-modified dopamine in the second dispersion;

[0065] (3) Add 1 m...

Embodiment 2

[0067] The preparation of embodiment 2 nano medicine

[0068] (1) Take 0.1 mL of paclitaxel with a concentration of 1.5 μg / mL and dissolve it in acetonitrile, add 1 mL of sodium polyphosphate solution with a concentration of 30 mg / mL to obtain the first mixed solution; The solution was added to the first mixed solution to obtain the second mixed solution, which was centrifuged after magnetic stirring at room temperature for 60 minutes, and the precipitate was ultrasonically redissolved to obtain the first dispersion, which contained paclitaxel (DTX) encapsulated calcium phosphate matrix;

[0069] (2) Add 10 mL of dopamine with a concentration of 2 mg / mL, adjust the pH to 9, centrifuge after 20 hours of magnetic stirring at room temperature, redissolve the precipitate and centrifuge, wash 4 times and redissolve the precipitate ultrasonically to obtain the second dispersion liquid. A calcium phosphate matrix containing surface-modified dopamine in the second dispersion;

[007...

Embodiment 3

[0072] The preparation of embodiment 3 nano medicine

[0073] (1) Dissolve 0.1 mL of camptothecin with a concentration of 0.5 μg / mL in dimethyl sulfoxide, and add 1 mL of sodium polyphosphate solution with a concentration of 10 mg / mL to obtain the first mixed solution; Calcium chloride solution per mL was added to the first mixed solution to obtain the second mixed solution, which was centrifuged after magnetic stirring at room temperature for 10 minutes, and the precipitate was ultrasonically redissolved to obtain the first dispersion, which contained Calcium phosphate matrix of resin;

[0074] (2) Add 10 mL of dopamine with a concentration of 0.5 mg / mL, adjust the pH to 7, centrifuge after magnetic stirring at room temperature for 10 h, redissolve the precipitate and centrifuge, wash twice, and redissolve the precipitate ultrasonically to obtain the second dispersion. A calcium phosphate matrix containing surface-modified dopamine in the second dispersion liquid;

[0075] ...

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Abstract

The invention provides a nano-medicine and its preparation method and application. The nano-medicine comprises a calcium phosphate matrix, a chemotherapeutic drug encapsulated in the calcium phosphate matrix, a photothermal agent modified on the surface of the calcium phosphate matrix, and a loaded An autophagy inhibitor in the pores of the photothermal agent. The nanomedicine of the present invention combines chemotherapy and hyperthermia, uses calcium phosphate as a rigid matrix, and encapsulates chemotherapeutic drugs and photothermal reagents in the calcium phosphate rigid matrix, and is loaded with autophagy inhibitors at the same time. By inhibiting autophagy, the Sensitize the effect of chemotherapy and hyperthermia, improve the curative effect on tumor treatment.

Description

technical field [0001] The invention belongs to the technical field of nano-medicine carrier materials, relates to a nano-medicine and its preparation method and application, in particular to a nano-medicine capable of chemotherapy and hyperthermia sensitization by inhibiting autophagy, and its preparation method and application. Background technique [0002] The limitation of cancer treatment lies in the single treatment method and the lack of effective chemotherapeutic drug targeting strategies, making it difficult to achieve significant curative effect. Chemotherapy is one of the most commonly used clinical treatment methods for breast cancer, but the currently used chemotherapeutic drugs lack effective carriers, have poor specificity, and are often accompanied by severe side effects. Photothermal therapy (PTT) is a cancer treatment method with broad application prospects. With the assistance of photothermal agents, energy induces cancer cell death through the phototherma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K47/02A61K47/18A61K31/337A61K41/00A61P35/00B82Y5/00
CPCA61K9/5115A61K9/5123A61K31/337A61K41/0052A61P35/00B82Y5/00A61K2300/00
Inventor 吴雁王绚
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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