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Polypeptide, application thereof and inhibitors or drugs or health products

A technology of health products and inhibitors, which is applied in the field of peptides and their applications and inhibitors or drugs or health products, which can solve the problems of changing treatment plans and not being able to cure Alzheimer's disease, so as to improve memory and have good application prospects Effect

Active Publication Date: 2020-02-28
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are currently no drugs or other treatment options that can cure Alzheimer's disease or alter its course

Method used

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  • Polypeptide, application thereof and inhibitors or drugs or health products
  • Polypeptide, application thereof and inhibitors or drugs or health products
  • Polypeptide, application thereof and inhibitors or drugs or health products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The preparation of embodiment 1 polypeptide Phe-Pro-Ser-Ile-Val-Gly-Arg-Pro

[0015] A method combining LC-MS / MS with Shotgun proteomics technology was used. Using sika deer antler as raw material, through enzymatic hydrolysis, centrifugation, purification and LC-MS / MS analysis, combined with the characteristics of structure-activity relationship, the peptides with inhibitory effects on DPP-Ⅳ and PEP were screened.

[0016] The specific method is as follows:

[0017] Freeze-dry fresh sika antler and crush it, add deionized water to make the antler concentration 33.3g / L, stir well, add trypsin with 0.5% antler mass (W / W), and enzymolyze at 40°C for 3 hours; enzymolysis At the end, pass the enzymatic solution through an 80-mesh sieve, and extract the residue once in the same way. Heat the two enzymatic hydrolysis solutions to 90°C for 15 minutes, filter through 8 layers of gauze and 200-mesh sieve successively, centrifuge the obtained filtrate at a speed of 10000g for 1...

Embodiment 2

[0021] Example 2 Detection of DPP-IV inhibitory activity of polypeptide Phe-Pro-Ser-Ile-Val-Gly-Arg-Pro

[0022] principle

[0023]For peptides with X-Pro and X-Ala at the N-terminal, DPP-IV can selectively excise the dipeptide, and GLP-1 has an X-Ala structure, so DPP-IV can cause more than 95% of GLP-1 to occur degradation. In this method, Gly-Pro-p-nitroanilide is used instead of GLP-1 as the simulated substrate of DPP-Ⅳ. After DPP-Ⅳ cuts off Gly-Pro, the generated p-nitroaniline has a characteristic absorption peak at 405nm, which can be calculated Inhibitory activity of DPP-IV.

[0024] experimental method:

[0025] The polypeptide Phe-Pro-Ser-Ile-Val-Gly-Arg-Pro used in the experiment was synthesized by Nanjing Jiepei Biotechnology Co., Ltd. with a purity of >95%. Dissolve the polypeptide Phe-Pro-Ser-Ile-Val-Gly-Arg-Pro in 100 mM Tris-HCl buffer (pH=8.0), add 25 μL of Phe-Pro-Ser-Ile-Val-Gly to a 96-well plate -Arg-Pro solution and 25μL 1.59mM Gly-pro-p-nitroanilide...

Embodiment 3

[0032] Example 3 Detection of PEP inhibitory activity of polypeptide Phe-Pro-Ser-Ile-Val-Gly-Arg-Pro

[0033] 1. Principle

[0034] PEP can specifically hydrolyze small molecular weight polypeptides at the carboxyl terminus of proline. Therefore, this method uses Z-Gly-Pro-4-nitroanilide as the substrate of PEP. After Z-Gly-Pro-4-nitroanilide is cut off by PEP to cut Z-Gly-Pro, yellow p-nitroanilide is produced, which has a characteristic at 405nm. Absorption peak, according to the change of absorbance at 405nm before and after adding the sample and substrate, the inhibitory activity of the sample for PEP can be calculated.

[0035] 2. Method

[0036] (1) Polypeptide Phe-Pro-Ser-Ile-Val-Gly-Arg-Pro solution: The polypeptide Phe-Pro-Ser-Ile-Val-Gly-Arg-Pro used in the experiment was synthesized by Nanjing Jiepei Biotechnology Co., Ltd., the purity >95%. Phe-Pro-Ser-Ile-Val-Gly-Arg-Pro was dissolved in 10 mM PBS buffer (10 mM PBS, 137 mM NaCl and 2.7 mM KCl, pH 7.0).

[003...

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Abstract

The invention relates to a polypeptide compound pheproserile valgly, which can inhibit the activity of DPP IV, prolylendopeptidase IV,lower blood sugar and fight Alzheimer's disease Argpro, whose amino acid sequence is pheproserievalglyargpro. The polypeptide pheproserievalglyargpro has DPP IV and PEP inhibitory activities, hypoglycemic and memory improving activities. It has a good application prospect as a health care product and drug lead compound for hypoglycemic and memory improvement as well as for the treatment of type II diabetes and anti Alzheimer's disease.

Description

technical field [0001] The present invention relates to polypeptide Phe-Pro-Ser-Ile-Val-Gly-Arg-Pro in the preparation of inhibiting dipeptidyl peptidase (DPP-IV, dipeptidyl peptidase IV), prolyl endonuclease (PEP, Prolyl Endopeptidase) and reducing Application in blood sugar, anti-Alzheimer's disease drugs and health care products. Background technique [0002] Diabetes is a syndrome of glucose, protein, and lipid metabolism disorders caused by absolute or relative insufficiency of insulin in the body. Currently, diabetes has become a global epidemic. According to the statistics of the World Health Organization, the incidence of diabetes is increasing at a certain rate every year. In 2016, the prevalence of diabetes in my country was 9.6%, and with the improvement of living standards, this figure is still rising sharply. [0003] Dipeptidyl peptidase IV (DPP-IV, dipeptidyl peptidase IV) is a serine protease whose substrate includes a polypeptide whose N-terminal second p...

Claims

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Application Information

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IPC IPC(8): C07K7/06A61K38/08A61P3/10A61P25/00A61P25/28A23L33/18
CPCC07K7/06A61P3/10A61P25/00A61P25/28A23L33/18A61K38/00A23V2002/00A23V2200/328A23V2200/322
Inventor 靳艳叶明亮晏嘉泽
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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