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Colorectal cancer marker and application thereof

A technology of colorectal cancer and markers, applied in the field of cancer, can solve the problems of high misdiagnosis rate and low patient survival rate, and achieve good accuracy, improved precision detection, and good sensitivity

Inactive Publication Date: 2020-03-10
SHENZHEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the diagnosis and treatment methods for colorectal cancer at home and abroad are mainly through surgical resection, radiotherapy, chemotherapy and other comprehensive treatments for patients who have been diagnosed. Lymph node metastasis and distant organ metastasis (such as: liver or lung, etc.), resulting in high misdiagnosis rate and low 5-year survival rate

Method used

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  • Colorectal cancer marker and application thereof
  • Colorectal cancer marker and application thereof
  • Colorectal cancer marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Material

[0034] Colorectal cancer tissue samples were obtained from colorectal cancer microarray tissue chip (CO803a, US Biomax), purchased from Xi'an Alina Biotechnology Co., Ltd., a total of 80 cases of colorectal tissue, including 39 cases of adenocarcinoma, signet ring cell carcinoma 1 and 40 matched paracancerous colon tissues. Among the cancerous tissues, 13 cases were older than or equal to 50 years old, and 27 cases were younger than 50 years old; 16 cases were males and 24 cases were females.

[0035] Detection method

[0036] The obtained colorectal cancer tissue samples and colorectal cancer paracancerous tissues were detected by one or a combination of qPCR, Western blot, and IHC (the specific methods are as follows) and analyzed and compared. The results are shown in Figure 1.

[0037] Method 1: Real-time fluorescent quantitative PCR (qPCR)

[0038] After each tissue was ground with liquid nitrogen, 1 mL of Trizol reagent was added, and after standing ...

Embodiment 2

[0045] Material

[0046] Colorectal cancer tissue samples were obtained from colorectal cancer microarray tissue chip (CO803a, US Biomax), purchased from Xi'an Alina Biotechnology Co., Ltd., a total of 80 cases of colorectal tissue, including 39 cases of adenocarcinoma, signet ring cell carcinoma 1 and 40 matched paracancerous colon tissues. Among the cancerous tissues, 13 cases were older than or equal to 50 years old, and 27 cases were younger than 50 years old; 16 cases were males and 24 cases were females. Classified according to clinical stage: 4 cases of colorectal cancer clinical stage I tissues, 21 cases of clinical stage II tissues, and 15 cases of clinical stage III tissues.

[0047] Detection method

[0048] The obtained colorectal cancer tissue sample and colorectal cancer paracancerous tissue were detected by one or more combination methods of qPCR, Western blot, and IHC (the specific method was the same as in Example 1) and analyzed and compared. The results we...

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Abstract

The invention relates to a colorectal cancer marker and an application thereof, and in particular to abnormal expression of a TAF1L gene of a colorectal cancer marker, and a detection method and an application of the colorectal cancer marker. The colorectal cancer marker comprises nucleic acid and / or protein with an abnormally expressed TAF1L gene, and detection of nucleic acid or / and proteins with abnormally expressed TAF1L gene can be realized by using a gene sequencing technology, a chip semi-quantitative detection technology, a qPCR quantitative detection technology, a Western blot semi-quantitative detection technology, an immunohistochemistry staining technology, an in-situ hybridization technology and a liquid biopsy technology, wherein the detection has good accuracy, high specificity and high sensitivity.

Description

technical field [0001] The invention belongs to the field of cancer, especially colorectal cancer. The invention relates to a colorectal cancer marker and its application, in particular to a colorectal cancer marker TAF1L protein and its detection method and application. Background technique [0002] Colorectal cancer (CRC) is one of the most common malignant tumors in humans. It is a common malignant tumor of the digestive system. The incidence rate is increasing year by year, ranking third among malignant tumors. Its onset is hidden and its prognosis is poor. . The occurrence and development of colorectal cancer can be divided into several stages such as abnormal hyperplasia, tumor in situ, malignant invasion, and distant metastasis. Because its etiology and pathogenesis have not been elucidated, there are no specific drugs and specific detection markers. However, early detection and early intervention can significantly improve the prognosis. At present, the diagnosis a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/574C12M1/34
CPCC12Q1/6886G01N33/57484G01N33/57419C12Q2600/118C12Q2600/156C12Q2600/158G01N2800/52
Inventor 王筠许特盛司潼李延鹏钟姗谭辉彪
Owner SHENZHEN UNIV