Application of vanillin as a redox mediator in improving the degradation rate of diethylstilbestrol by laccase

Abstract

The invention discloses the application of vanillin as a redox medium in improving the degradation rate of diethylstilbestrol by laccase, and the vanillin concentration is 0.01-1mmol / L. Also disclosed is a method for improving the degradation rate of diethylstilbestrol by laccase with vanillin as a redox medium, comprising the following steps: Step 1: dissolving laccase in acetic acid-sodium acetate buffer to prepare laccase mother liquor; dissolving diethylstilbestrol in methanol to prepare into a diethylstilbestrol solution; dissolving vanillin in acetic acid-sodium acetate buffer to prepare a vanillin solution; step 2: after mixing the prepared laccase mother liquor and vanillin solution into a laccase-vanillin solution, mix it with the prepared diethylstilbestrol solution Mixed in the reaction system, placed in a shaking incubator for reaction; Step 3: After half an hour of reaction, use ethyl acetate to extract to terminate the diethylstilbestrol reaction and complete the degradation. The invention uses vanillin as a reducing medium, which can greatly improve the degradation rate of DES degraded by laccase and reduce the degradation cost.

Description

Technical field[0001]The present invention relates to the application of environmental pollution treatment techniques, and in particular, a vanillal aldehyde as an oxidative medium is used in increasing laccase degradation of hexheol degradation rate.Background technique[0002]Ethylstilbestrol (DES) is a synthetic non-steroidal estrogen or ischarotone having a stronger estrogen activity than estradiol (Korach et al., 1978; SHANG et al., 2014). Since 1938 by British chemist, it was originally used to treat gastrointestinal discomfort, dizziness and redness of skin. Later, it was widely used in the medical community as a prescription drug for preventing abortion, and is also used to treat advanced prostate cancer, breast cancer, ovarian function, amenorrhea, uterine development, and functional uterine bleeding diseases (Goyal et al., 2001; Grenader et al., 2014; Koong etal., 2014). In 1971 hexaperphol was disabled (HE et al., 2002; Tournaire et al., 2015., 2018; Troisi et al., 2016). H...

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Problems solved by technology

[0003] Lin et al. used ozone oxidation to degrade DES, but the effect was not significant (Lin et al., 2009)

Xu et al. studied the UV conversion of diethylstilbestrol and its persistent pollution to surface water under sunlight, and found that DES can be photocatalytically degraded, but also found that DES is a photochromic compound whose UV-induced intermediate can Conversion back to DES in sunlight, which apparently slows down the photodegradation of DES (Xu et al., 2017)

As a kind of fat-soluble compound DES is not easily degraded in the environment, the traditional treatment methods to degrade this kind of phenolic organic pollutants are not only expensive but also ineffective (Snyder et al., 2003; Tay et al., 2009)

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