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Heat treatment clarification method of industrial enzyme liquid

An industrial, enzyme solution technology, applied in the direction of biochemical equipment and methods, enzymes, enzymes, etc., can solve the problems of water pollution, easily polluted water, stimulation, etc., and achieve the effect of simple process, good promotion value and low cost

Pending Publication Date: 2020-03-17
ENZYMASTER NINGBO BIO ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The authorized invention patent uses a mixed flocculant composed of aluminum sulfate, ferrous sulfate and neutral polyacrylamide. Among them, aluminum sulfate has a certain stimulating effect on human eyes and mucous membranes, and is easy to pollute water bodies; The respiratory tract is irritating, inhalation can cause coughing and shortness of breath, serious ingestion can be fatal, and it is harmful to the environment and can pollute water bodies; polyethyleneimine is highly toxic and harmful to the environment

Method used

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  • Heat treatment clarification method of industrial enzyme liquid
  • Heat treatment clarification method of industrial enzyme liquid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A heat treatment clarification method for industrial enzyme liquid, comprising the following steps:

[0044] (1) Select the target protein for cultivation and fermentation:

[0045] Dehydrogenase is selected as the target protein, fermented according to the existing fermentation production process to obtain a fermentation liquid, and then the fermentation liquid is subjected to cell crushing treatment through a homogenizer to obtain a cell crushing liquid;

[0046] (2) heat treatment clarification:

[0047] Carry out heat treatment and clarification to the cell disruption solution obtained in step (1), and the heat treatment clarification is:

[0048] like figure 1 As shown, the cell disruption solution is passed through a serpentine tube 200 placed in the water bath heating tank 100 at a constant speed (the diameter of the pipe is 5.5mm, and the total length is 1.4m), and the constant temperature circulating hot water 101 is passed into the water bath heating tank 10...

Embodiment 2

[0053] A heat treatment clarification method for industrial enzyme liquid, comprising the following steps:

[0054] (1) Select the target protein for cultivation and fermentation:

[0055] Dehydrogenase is selected as the target protein, fermented according to the existing fermentation production process to obtain a fermentation liquid, and then the fermentation liquid is subjected to cell crushing treatment through a homogenizer to obtain a cell crushing liquid;

[0056] (2) heat treatment clarification:

[0057] Carry out heat treatment and clarification to the cell disruption solution obtained in step (1), and the heat treatment clarification is:

[0058] Pass the cell disruption liquid through a U-shaped pipe placed in a water bath heating tank at a constant speed (the diameter of the pipe is 5.5mm, and the total length is 1.4m), control the temperature in the U-shaped pipe to 80°C, and control the flow rate of the cell disruption liquid so that Its passage time in the U...

Embodiment 3

[0063] A heat treatment clarification method for industrial enzyme liquid, comprising the following steps:

[0064] (1) Select the target protein for cultivation and fermentation:

[0065] Dehydrogenase is selected as the target protein, fermented according to the existing fermentation production process to obtain a fermentation liquid, and then the fermentation liquid is subjected to cell crushing treatment through a homogenizer to obtain a cell crushing liquid;

[0066] (2) heat treatment clarification:

[0067] Carry out heat treatment and clarification to the cell disruption solution obtained in step (1), and the heat treatment clarification is:

[0068] Pass the cell disruption liquid through a section of W-shaped pipe placed in a water bath heating tank at a constant speed (the diameter of the pipe is 5.5mm, and the total length is 1.4m). Its passage time in the W-shaped pipeline is 24s;

[0069] (3) Post-processing:

[0070] Centrifuge the heat-treated and clarified...

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Abstract

The invention provides a heat treatment clarification method of an industrial enzyme liquid. The method comprises the following steps: (1) target protein selection, culture and fermentation: selectinga target protein, fermenting the protein according to the existing fermentation production process to obtain a fermentation liquid, and carrying out cell disruption treatment on the fermentation liquid to obtain a cell disruption liquid, (2) heat treatment clarification: carrying out heat treatment clarification on the cell disruption liquid obtained in the step (1), and (3) post-treatment: carrying out centrifugal treatment on the liquid subjected to heat treatment clarification in the step (2), and collecting the clear liquid to obtain the industrial enzyme clarified liquid of the target protein. The method has the advantages of simple process, low cost and environmental protection, the enzyme activity loss of the target protein in the clarified enzyme liquid is not more than 10%, the turbidity is lower than 500NTU, and the clarified enzyme liquid can pass through 0.2 micromoles per filter of a filtering membrane.

Description

technical field [0001] The invention belongs to the technical field of industrial enzyme preparation production, and in particular relates to a heat treatment clarification method for industrial enzyme liquid, which is suitable for a clarification process for large-scale preparation of industrial enzyme liquid. Background technique [0002] Enzymes are biological catalysts produced by living cells that catalyze specific biochemical reactions. Enzyme preparation is a biological product with catalytic function after enzyme purification and processing. It is mainly used to catalyze various chemical reactions in the production process. It has high catalytic efficiency, high specificity, mild action conditions, reduced energy consumption, and reduced chemical Pollution and other characteristics, its application fields are all over food, textile, feed, lotion, paper, leather, medicine, energy development, environmental protection and so on. [0003] The enzyme preparation industr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/00
CPCC12N9/00
Inventor 张迎新纪摇摇孙磊陈海滨孙保国黄勇开
Owner ENZYMASTER NINGBO BIO ENG CO LTD
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