Fermentation culture method based on hirsutella sinensis metabolic pathway

A technology of Chinese Trichosporium, fermentation culture, applied in the direction of microorganism-based methods, fermentation, biochemical equipment and methods, etc., to achieve the effect of increasing the content

Inactive Publication Date: 2020-03-20
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Currently, there are no methods based on metabolomics and metabolic pathway analysis in the application of Chinese hairy sp.

Method used

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  • Fermentation culture method based on hirsutella sinensis metabolic pathway
  • Fermentation culture method based on hirsutella sinensis metabolic pathway
  • Fermentation culture method based on hirsutella sinensis metabolic pathway

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The fermentation production method based on the metabolic pathway of Chinese hairy sporosum comprises the following steps:

[0044] (1) Hirsutella sinensis ZJB18002 seed culture:

[0045] Get a piece of gray spore colony from the slant culture medium and inoculate it in a bottle of 250mL Erlenmeyer flask containing 50mL seed medium, the culture temperature is 16°C, the shaker speed is 150r / min, cultivate for 96h, and obtain the seed liquid; the seed culture The basis is as described in the comparative example.

[0046] (2) Hirsutella sinensis ZJB18002 fermentation culture:

[0047] Add 40g / L of trehalose, maltose and sorbitol, and 4mmol / L of β-alanine, L-proline, L-leucine, L-methionine, L-valine to the fermentation medium Amino acid, L-cysteine, trans-4-hydroxy-L-proline, L-serine, inoculate 2mL seed solution into 50mL fermentation medium, the fermentation vessel is a 250mL Erlenmeyer flask, at 15°C, Under the condition of 150r / min, shaker culture was carried out fo...

Embodiment 2

[0050] The fermentation production method based on the metabolic pathway of Chinese hairy sporosum comprises the following steps:

[0051] (1) Hirsutella sinensis ZJB18002 seed culture:

[0052] Get a piece of gray spore colony from the slant culture medium and inoculate it in a bottle of 250mL Erlenmeyer flask containing 50mL seed medium, the culture temperature is 16°C, the shaker speed is 150r / min, cultivate for 96h, and obtain the seed liquid; the seed culture The basis is as described in the comparative example.

[0053] (2) Hirsutella sinensis ZJB18002 fermentation culture:

[0054] Add 40g / L of trehalose, maltose and sorbitol, and 4mmol / L of β-alanine, L-proline, L-leucine, L-methionine, L-valine to the fermentation medium Amino acid, L-cysteine, trans-4-hydroxy-L-proline, L-serine, inoculate 2mL seed solution into 50mL fermentation medium, the fermentation vessel is a 250mL Erlenmeyer flask, at 15°C, Under the condition of 150r / min, shaker culture was carried out fo...

Embodiment 3

[0057] The fermentation production method based on the metabolic pathway of Chinese hairy sporosum comprises the following steps:

[0058] (1) Hirsutella sinensis ZJB18002 seed culture:

[0059] Get a piece of gray spore colony from the slant culture medium and inoculate it in a bottle of 250mL Erlenmeyer flask containing 50mL seed medium, the culture temperature is 16°C, the shaker speed is 150r / min, cultivate for 96h, and obtain the seed liquid; the seed culture The basis is as described in the comparative example.

[0060] (2) Hirsutella sinensis ZJB18002 fermentation culture:

[0061] Add 40g / L of trehalose, maltose and sorbitol, and 4mmol / L of β-alanine, L-proline, L-leucine, L-methionine, L-valine to the fermentation medium Amino acid, L-cysteine, trans-4-hydroxy-L-proline, L-serine, inoculate 2mL seed solution into 50mL fermentation medium, the fermentation vessel is a 250mL Erlenmeyer flask, at 15°C, Under the condition of 150r / min, shaker culture was carried out fo...

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Abstract

The invention relates to a culture method based on a hirsutella sinensis metabolic pathway. The method comprises the following steps: inoculating the hirsutella sinensis strain into a fermentation culture medium, wherein a key differential metabolite is added into the fermentation culture medium, performing culture at 10-25 DEG C and 100-250 rpm for 216-240 h, performing filtering, oven-drying andgrinding into a powder on a fermentation product after the fermentation is completed, and then extracting cordycepic acid and cordycepin. The method can effectively increase the content of main active compounds cordycepic acid and cordycepin through adding various key differential metabolites before fermentation culture of hirsutella sinensis. As can be seen from the embodiment of the invention,the addition of sorbitol in the culture medium can increase the content of cordycepic acid from 59.28 mg/g to 149.38 mg/g; and the addition of L-proline or trans-4-hydroxy-L-proline can increase the content of cordycepin from 0.1103 mg/g to 0.1622 mg/g or 0.1867 mg/g, respectively.

Description

[0001] (1) Technical field [0002] The invention relates to a fermentation and cultivation method based on the metabolic pathway of Trichosporum sinensis, belonging to the technical field of microbial fermentation engineering. [0003] (2) Background technology [0004] Cordyceps sinensis (Cordyceps sinensis (Berk.) Sacc.) is a complex of Cordyceps sinensis parasitizing on the larvae of Lepidoptera (Lepidoptera) bat moth (Hepialus armoricanus Oberthur) larvae and larval corpses (including subunits and worm bodies ). Cordyceps sinensis is a kind of precious traditional fungal medicinal resources, which has the characteristics of diverse metabolites and biological activities, and has shown great application and development prospects in the field of biomedicine. Cordyceps sinensis has attracted much attention because of its wide and obvious medicinal effects, and is highly respected all over the world. Chinese medicine believes that Cordyceps sinensis enters the two meridians o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/38C12P19/40C12P7/18C12R1/645
CPCC12N1/14C12N1/38C12P7/18C12P19/40
Inventor 柳志强门晓慧张博郑裕国
Owner ZHEJIANG UNIV OF TECH
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