Millettia speciosa champ polysaccharide, preparation method and application thereof in antibacterial aspect

A technology of oxalicum and polysaccharide, which is applied in the field of natural active products, can solve the problems such as the lack of research reports on the separation, purification and activity evaluation of oxalicum polysaccharides, and achieves extensive development value and the effect of inhibiting growth and reproduction.

Active Publication Date: 2020-03-24
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current research mainly focuses on the extraction and activity evaluation of flavonoids in the root of Niu Da...

Method used

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  • Millettia speciosa champ polysaccharide, preparation method and application thereof in antibacterial aspect
  • Millettia speciosa champ polysaccharide, preparation method and application thereof in antibacterial aspect
  • Millettia speciosa champ polysaccharide, preparation method and application thereof in antibacterial aspect

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Embodiment 1

[0063] Embodiment 1: the preparation of bovine herbaceous polysaccharide MSCP1 and MSCP2

[0064] In the present invention, the separation and purification process of bovine vigorously polysaccharide is as follows: figure 1 Shown:

[0065] (1) Preparation of Niu Dali crude extract: Clean the root of Niu Dali (produced in Fengkai County, Zhaoqing City, Guangdong Province, China), cut into slices, dry (60°C for 15 hours), pulverize, pass through 100 Mesh sieve to obtain the root block powder of Niu Dali; use the hot water reflux method to extract the root block powder of Niu Dali, the conditions are temperature 80°C, material-liquid ratio 1:10, extraction time 1h; after the extraction, centrifuge to take the supernatant, Repeat the extraction twice according to the above conditions, combine the supernatants, concentrate under reduced pressure at 55°C to 1 / 4 of the volume, and obtain the crude extract of bovine herbaceous polysaccharide;

[0066] (2) Preparation of the crude po...

Embodiment 2

[0070] Embodiment 2: the preparation of bovine herbaceous polysaccharide MSCP1 and MSCP2

[0071] (1) Preparation of Niu Dali crude extract: Clean the root of Niu Dali (produced in Fengkai County, Zhaoqing City, Guangdong Province, China), cut into slices, dry (60°C for 15 hours), pulverize, pass through 100 Mesh sieve to obtain the root block powder of Niu Dali; use the hot water reflux method to extract the root block powder of Niu Dali, the conditions are temperature 90°C, material-liquid ratio 1:20, extraction time 2h; centrifuge after leaching to take the supernatant, Repeat the extraction twice according to the above conditions, combine the supernatants, concentrate under reduced pressure at 65°C to 1 / 4 of the volume, and obtain the crude extract of bovine herbaceous polysaccharide;

[0072] (2) Preparation of the crude polysaccharide of bovine vigorously: in order to remove the pigment and protein, add 300mL of crude polysaccharide of bovine vigorously to the AB-8 macro...

Embodiment 3

[0076] Embodiment 3: the preparation of bovine herbaceous polysaccharide MSCP1 and MSCP2

[0077] (1) Preparation of Niu Dali crude extract: Clean the root of Niu Dali (produced in Fengkai County, Zhaoqing City, Guangdong Province, China), cut into slices, dry (60°C for 15 hours), pulverize, pass through 100 Mesh sieve to obtain the root block powder of Niu Dali; use the hot water reflux method to extract the root block powder of Niu Dali, the conditions are temperature 100°C, material-liquid ratio 1:30, extraction time 3h; centrifuge after leaching to take the supernatant, Repeat the extraction twice according to the above conditions, combine the supernatants, concentrate under reduced pressure at 75°C to 1 / 4 of the volume, and obtain the crude extract of bovine herbaceous polysaccharide;

[0078] (2) Preparation of the crude polysaccharide of bovine vigorously: in order to remove the pigment and protein, add 300mL of crude polysaccharide of bovine vigorously to the AB-8 macr...

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Abstract

The invention discloses a millettia speciosa champ polysaccharide, a preparation method and application thereof in antibacterial aspect. The method includes the steps of: (1) cleaning millettia speciosa champ root tubers, performing slicing, drying, crushing and sieving, and then carrying out hot water reflux extraction to obtain a millettia speciosa champ polysaccharide crude extracted liquid; (2) removing pigment from the millettia speciosa champ polysaccharide crude extracted liquid by macroporous resin, removing residual protein by a Sevag reagent, and then conducting alcohol precipitationto obtain millettia speciosa champ crude polysaccharide; and (3) adding water into the millettia speciosa champ crude polysaccharide to prepare a millettia speciosa champ crude polysaccharide solution, then adding the millettia speciosa champ crude polysaccharide solution into a DEAE-52 cellulose anion exchange column, sequentially conducting elution with distilled water and a 0.2mol/L NaCl solution, and respectively collecting eluent to obtain millettia speciosa champ polysaccharides MSCP1 and MSCP2. The millettia speciosa champ polysaccharide prepared according to the invention has certaininhibition effect on gram-negative bacteria, especially escherichia coli and salmonella, therefore the millettia speciosa champ polysaccharide can be used for developing antibacterial materials or antibacterial agents.

Description

technical field [0001] The invention relates to the field of natural active products, in particular to a bovine herbaceous polysaccharide, its preparation method and its antibacterial application. Background technique [0002] Polysaccharides are an important class of biological macromolecules. In recent years, polysaccharides isolated from plants, animals, and microorganisms have shown biological activities such as anti-oxidation, anti-tumor, anti-inflammation, hypoglycemia, and immune regulation, so they have received extensive attention. Niu Dali is the main variety of Nanyao. It belongs to the Papilionaceae Papilionaceae plant Spathiphyllum beautiful. It is mainly distributed in the southern part of my country and some countries in Southeast Asia. Niu Dali, as a kind of Chinese herbal medicine with both medicine and food, has the functions of treating arthritis and bronchitis, protecting the liver, strengthening muscles and bones, nourishing deficiency and nourishing lu...

Claims

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Application Information

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IPC IPC(8): C08B37/00A23L33/125A61K31/715A61P31/04
CPCA23V2002/00A61K31/715A23L33/125A61P31/04C08B37/0003C08B37/006A23V2200/324A23V2250/51Y02A50/30
Inventor 娄文勇黄智罗思媛陈曦
Owner SOUTH CHINA UNIV OF TECH
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