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A Straw Saprophytic Fungus and Its Application

A saprophytic bacteria and straw technology, applied in the field of microorganisms, can solve problems such as refractory degradation of straw, and achieve the effect of improving soil and promoting vegetation growth.

Active Publication Date: 2021-08-10
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problem that the existing straw is difficult to degrade, and provides a straw saprophytic fungus and its application

Method used

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  • A Straw Saprophytic Fungus and Its Application
  • A Straw Saprophytic Fungus and Its Application
  • A Straw Saprophytic Fungus and Its Application

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Experimental program
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specific Embodiment approach 1

[0021] Specific implementation mode 1: In this implementation mode, the straw saprophyte is Pythium ultimum TL1, which is preserved in the General Microorganism Center (CGMCC) of the China Microbiological Culture Collection Management Committee (CGMCC), and the storage address is No. 1, Beichen West Road, Chaoyang District, Beijing. No. 3, the deposit date is October 18, 2019, and the deposit number is CGMCC No.18590.

[0022] The Pythium ultimum TL1 of this embodiment can grow in the form of mycelia in solid potato sucrose medium.

[0023] The color of colonies of Pythium ultimum TL1 in this embodiment is white, the hyphae are white, carpet-like and scattered, and the spores are conidia. The morphology of strain TL1 on the PDA plate is as follows figure 1 shown.

specific Embodiment approach 2

[0024] Specific embodiment two: the acquisition method of the ultimate Pythium ultimum (Pythium ultimum) TL1 in this embodiment is:

[0025] Preliminary screening: Using the plate separation method, select samples from the experimental field of the Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun City, Jilin Province, cut the rotten rice samples from the stem and leaves, cut them into 2cm, rinse them in 45ml sterile water for several times, and place them After drying on a sterilized filter paper sheet, place the 2cm stem and leaf samples on the potato solid medium, and place them upside down in a 25°C incubator for 2-3 days to observe the growth of the colony and the formation of the transparent circle.

[0026] Potato solid medium: Peel 200g of potatoes, cut into pieces, boil in 500ml of water for 10min, filter the filtrate with gauze, add 10g of sucrose, 10g of agar powder, add distilled water to 1000ml, sterilize under high pressure s...

specific Embodiment approach 3

[0030] Embodiment 3: Pythium ultimum TL1 can degrade rice straw.

[0031] Straw degradation experiments were carried out on the strain TL1. An equal volume of dried rice straw was added to 30 ml of water, placed in a tissue culture dish, and sterilized by high-pressure steam at 115° C. for 30 minutes. Then inoculate 1 cm directly punched PDA bacterium block of bacterial strain TL1 under aseptic condition. The control group was not inoculated. Placed in a 25°C incubator for 30 days. The picture of the rapid propagation of strain TL1 in the rice straw matrix plate is as follows image 3 shown. The weight of the straw before and after degradation was weighed with an electronic balance, and the results are shown in Table 1. It was found that the straw weight loss rate of the inoculated strain was 0.049%, while that of the uninoculated straw was 0.014%. It shows that Pythium ultimum TL1 can degrade rice straw.

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Abstract

A straw saprophytic fungus and its application relate to the field of microorganisms, in particular a straw saprophytic fungus and its application. It is to solve the problem that the existing straw is difficult to degrade. The fungus is Pythium ultimum TL1, and it is preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures. The preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation date is October 18, 2019. , the deposit number is CGMCC No.18590. Pythium ultima TL1 can degrade straw, can promote rice straw to produce humic acid in the process of rice straw decomposition, and can promote 1g of rice straw to produce humic acid by 0.005g. The invention is applied to the field of straw degradation.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a straw saprophytic fungus and its application. Background technique [0002] Rice is the main food crop in my country and also the most important economic crop. After rice harvest, a large amount of straw remains, which can be used as a rural resource; however, rice straw cannot be effectively returned to the field in many areas, and it is burned for heating, which has a serious impact on environmental pollution. [0003] There is a lack of research on the impact of decomposing fungi on humic acid during the degradation of rice straw, and there is no effective technical system support. At present, it is imminent to isolate and identify microorganisms in the process of rice straw degradation. Contents of the invention [0004] The invention aims to solve the problem that the existing straw is difficult to degrade, and provides a straw saprophytic fungus and its application. [...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P1/02C05F11/02C12R1/645
CPCC05F11/02C12P1/02C12N1/145C12R2001/645
Inventor 田磊吉丽林晓龙田春杰
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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