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Preparation method and application of ultraviolet-cured hyaluronic acid hydrogel with adjustable crosslinking degree

A technology of hyaluronic acid and hydrogel, applied in medical science, prosthesis, etc., can solve the problems of slow polymerization of biomass hydrogel, small molecule residue, difficult to remove, etc., and achieve controllable water absorption rate and strength, Good biocompatibility and controllable cross-linking effect

Active Publication Date: 2020-03-31
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the introduction of other chemical molecules and the complicated preparation process will inevitably lead to the residue of small molecules
These molecules are difficult to remove, and most of them are toxic to cells
In addition, traditional biomass hydrogels have slow polymerization speed, low molding precision, and difficult to effectively control the micron size, which limits their wide application in biology.

Method used

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  • Preparation method and application of ultraviolet-cured hyaluronic acid hydrogel with adjustable crosslinking degree
  • Preparation method and application of ultraviolet-cured hyaluronic acid hydrogel with adjustable crosslinking degree
  • Preparation method and application of ultraviolet-cured hyaluronic acid hydrogel with adjustable crosslinking degree

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A preparation method of UV-curable hyaluronic acid hydrogel with adjustable crosslinking degree, said method comprising the following steps:

[0034] (1) Dissolve hyaluronic acid with a molecular weight of 100,000 in deionized water (1wt%) to form a uniform and transparent solution, add 1 volume of DMF, stir mechanically, cool to 3°C, and maintain the stirring and temperature conditions; Slowly add methacrylic anhydride with a molar ratio of 1.2 times (1 drop per second); then adjust the pH value of the mixture to 8 with 0.4M sodium hydroxide solution; after reacting for 10 hours, add 0.5M sodium chloride solid Continue stirring for 0.5h; use ethanol as a precipitating agent to obtain a white flocculated precipitate; the precipitate is washed with water and ethanol mixture (3 / 7, 1 / 4, 1 / 9 volume ratio); finally, the product is dissolved in deionized water (1wt %), dialyzed for 2 days, and freeze-dried to obtain a white spongy solid, i.e. methacrylated hyaluronic acid (HA...

Embodiment 2

[0038] A preparation method of UV-curable hyaluronic acid hydrogel with adjustable crosslinking degree, said method comprising the following steps:

[0039] (1) Dissolve hyaluronic acid with a molecular weight of 100,000 in deionized water (2wt%) to form a uniform and transparent solution, add 1.2 times the volume of DMF, stir mechanically, cool to 3.5°C, and maintain the stirring and temperature conditions; Slowly add methacrylic anhydride with a molar ratio of 1.4 times (2 drops per second); then adjust the pH value of the mixture to 8.5 with 0.5M sodium hydroxide solution; after reacting for 11 hours, add 0.5M sodium chloride solid Continue stirring for 0.8h; use ethanol as a precipitant to obtain a white flocculated precipitate; the precipitate is washed with water and ethanol mixture (3 / 7, 1 / 4, 1 / 9 volume ratio); finally, the product is dissolved in deionized water (1.5 wt%), dialyzed for 3 days, and freeze-dried to obtain a white spongy solid, i.e. methacrylated hyaluronic...

Embodiment 3

[0043] A preparation method of UV-curable hyaluronic acid hydrogel with adjustable crosslinking degree, said method comprising the following steps:

[0044] (1) Dissolve hyaluronic acid with a molecular weight of 100,000 in deionized water (3wt%) to form a uniform and transparent solution, add 1.5 times the volume of DMF, stir mechanically, cool to 4°C, and maintain the stirring and temperature conditions; Slowly add methacrylic anhydride with a molar ratio of 1.5 times (3 drops per second); then adjust the pH value of the mixture to 9 with 0.6M sodium hydroxide solution; after reacting for 12 hours, add 0.5M sodium chloride solid Continue stirring for 1h; use ethanol as a precipitant to obtain a white flocculated precipitate; the precipitate is washed with water and ethanol mixture (3 / 7, 1 / 4, 1 / 9 volume ratio); finally, the product is dissolved in deionized water (2wt% ), dialyzed for 4 days, and freeze-dried to obtain a white spongy solid, i.e. methacrylated hyaluronic acid ...

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Abstract

The invention provides a preparation method of ultraviolet-cured hyaluronic acid hydrogel with an adjustable crosslinking degree. By controlling the content of a cross-linking agent, the cross-linkingdegree and the water absorption rate of the hydrogel can be effectively adjusted. The prepared hydrogel is subjected to a rheological test and a compression test. Results show that the hydrogel can be quickly cured after being initiated by ultraviolet light; and the mechanical strength of the hydrogel reaches 70 kpa, which is close to the strength of blood vessels. In addition, the hydrogel has high forming precision, and micron-scale flow channels can be perfectly etched again. The hyaluronic acid hydrogel is also very effective in promoting the release of the vascular endothelial growth factor (VEGF).

Description

technical field [0001] The invention relates to a preparation method and application of an ultraviolet light-cured hyaluronic acid hydrogel with adjustable crosslinking degree. Background technique [0002] Biomass hydrogels have been greatly developed in wound healing, tissue scaffolding and drug release due to their advantages of degradability, green source and high biocompatibility. However, the introduction of other chemical molecules and the complicated preparation process will inevitably lead to the residue of small molecules. These molecules are difficult to remove, and most of them are toxic to cells. In addition, traditional biomass hydrogels have slow polymerization speed, low molding precision, and difficult to effectively control the micron size, which limits their wide application in biology. [0003] Hyaluronic acid is a biomaterial widely present in the extracellular matrix (ECM). It is a degradable and highly biocompatible natural linear polymer that promo...

Claims

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Application Information

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IPC IPC(8): C08J3/24C08J3/075C08B37/08A61L27/20A61L27/50A61L27/52A61L27/58
CPCC08J3/24C08J3/075C08B37/0072A61L27/20A61L27/50A61L27/52A61L27/58C08J2305/08C08L5/08
Inventor 陈枫张倩敏魏晓娟刘震杰钟明强
Owner ZHEJIANG UNIV OF TECH
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