A kind of collagen cross-linking agent composition and its application

A collagen and cross-linking agent technology, applied in the field of medical supplies, can solve problems such as the entry of toxic chemicals, and achieve the effects of preventing premature degradation, avoiding calcification and immune reactions, and reducing reactions between different species

Active Publication Date: 2021-05-18
南京中富先农生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, exogenous toxic chemicals enter the collagen

Method used

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  • A kind of collagen cross-linking agent composition and its application
  • A kind of collagen cross-linking agent composition and its application
  • A kind of collagen cross-linking agent composition and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The preparation of embodiment 1 crosslinking agent composition

[0040] The present embodiment has prepared the cross-linking agent composition solution of following prescription

[0041] Composition 1:

[0042] Genipin 0.1mg / ml, 0.1mg / ml 5-aminolevulinic acid, 0.05mol / L sodium citrate, 0.02mg / ml imidazole, PBS as buffer system, pH 8.0.

[0043] Composition 2:

[0044] 0.1mg / ml oxidized sodium alginate, 0.1mg / ml methylene blue, 0.05mol / L sodium citrate, 0.02mg / ml imidazole, PBS as buffer system, pH7.5.

[0045] Composition 3:

[0046] 50mg / ml oxidized sodium alginate, 20mg / ml methylene blue, 0.8mol / L potassium citrate, PBS as buffer system, pH7.0.

[0047] Control 1:

[0048] Genipin 0.2mg / ml, with PBS as the buffer system, pH7.5.

[0049] Control 2:

[0050] 0.2mg / ml 5-aminolevulinic acid, with PBS as the buffer system, pH7.0.

[0051] Control 3:

[0052]0.1mg / ml oxidized sodium alginate, 0.1mg / ml methylene blue, with PBS as the buffer system, pH7.5.

Embodiment 2

[0053] The acquisition of embodiment 2 collagen biomaterials

[0054] 2.1 Sclera

[0055] New Zealand white rabbits were killed by air embolism, the eyeballs were taken out, and the sclera was separated. After being soaked in dodecyl dimethyl benzyl ammonium bromide solution, then placed in polyethylene glycol octyl phenyl ether solution for incubation, placed in trypsin and EDTA mixed solution for incubation, and placed Incubate in a mixed solution of DNase-1 and RNase-A to obtain decellularized sclera tissue. Slices were made into 1cm×1cm. Obtain the decellularized material.

[0056] 2.2 Vascular tissue

[0057] Fresh and suitable bovine veins were selected and decellularized by a multi-step detergent-enzyme digestion method. Soak in 0.25% trypsin solution for 18 hours, then place in 0.1% SDS for 12 hours, then immerse the tissue in 0.25% trypsin solution for 12 hours, and finally treat it in Dispase at 25°C for 12 hours, and finally place the decellularized cow neck ...

Embodiment 3

[0060] Embodiment 3 cross-linking reaction

[0061] The decellularized material slices obtained in Example 2 were respectively soaked in 20 mL of the solution of Example 1, and put into an 80 mL container. Maintain the temperature of the equilibrium system at 15°C, the pH at 7.5-7.8, and soak for 60 minutes. Subsequently, under the osmotic pressure of 310-330 Osm, the cross-linking reaction was carried out for 60 minutes under the condition of 1000w incandescent light irradiation and oxygen flow. Finally, with 0.25M Na 2 CO 3 / NaHCO 3 buffer solution to adjust the pH of the reaction system to 7, soak and shake for 1 hour, and seal and store for later use.

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Abstract

The invention relates to a collagen cross-linking agent composition and application thereof. The composition comprises photosensitive crosslinking agent, chemical crosslinking agent, citrate and imidazole. The cross-linking agent composition can make the chemical cross-linking and photo-oxidative cross-linking of the decellularized collagen proceed simultaneously and play a synergistic effect, thereby improving the efficiency of the cross-linking reaction and obtaining higher-quality cross-linked collagen medical materials .

Description

technical field [0001] This application relates to the field of medical supplies, in particular to a collagen crosslinking agent composition and its application. Background technique [0002] Collagen is the most widely distributed protein in mammals, easy to extract, and has good biodegradability and biocompatibility. Collagen is a scaffold material for cell growth, which can promote cell adhesion and induce cell growth and differentiation. It is widely used in tissue engineering research and in the medical field. [0003] Collagen has been widely used because of its excellent low immune activity, good biocompatibility, and biodegradability, but unmodified collagen often has poor thermal stability, fast degradation rate, and low mechanical strength. Variability and other shortcomings, in many cases can not meet the requirements of use. Therefore, the method of modifying it is usually used to effectively improve the thermal stability and mechanical properties of collagen, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08K5/00C08K5/092C08K5/3445C08L5/04C08J3/24C08L89/00A61K47/42A61L24/00A61L24/10A61L27/24A61L27/54A61L27/60
CPCA61K47/42A61L24/0015A61L24/0036A61L24/102A61L27/24A61L27/54A61L27/60A61L2300/252A61L2300/412A61L2400/04A61L2430/02A61L2430/16A61L2430/40C08J3/243C08J2389/00C08J2405/04C08K5/0025C08K5/092C08K5/3445C08L5/04
Inventor 戴东升张建伟
Owner 南京中富先农生物科技有限公司
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