A kind of collagen cross-linking agent composition and its application
A collagen and cross-linking agent technology, applied in the field of medical supplies, can solve problems such as the entry of toxic chemicals, and achieve the effects of preventing premature degradation, avoiding calcification and immune reactions, and reducing reactions between different species
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0039] The preparation of embodiment 1 crosslinking agent composition
[0040] The present embodiment has prepared the cross-linking agent composition solution of following prescription
[0041] Composition 1:
[0042] Genipin 0.1mg / ml, 0.1mg / ml 5-aminolevulinic acid, 0.05mol / L sodium citrate, 0.02mg / ml imidazole, PBS as buffer system, pH 8.0.
[0043] Composition 2:
[0044] 0.1mg / ml oxidized sodium alginate, 0.1mg / ml methylene blue, 0.05mol / L sodium citrate, 0.02mg / ml imidazole, PBS as buffer system, pH7.5.
[0045] Composition 3:
[0046] 50mg / ml oxidized sodium alginate, 20mg / ml methylene blue, 0.8mol / L potassium citrate, PBS as buffer system, pH7.0.
[0047] Control 1:
[0048] Genipin 0.2mg / ml, with PBS as the buffer system, pH7.5.
[0049] Control 2:
[0050] 0.2mg / ml 5-aminolevulinic acid, with PBS as the buffer system, pH7.0.
[0051] Control 3:
[0052]0.1mg / ml oxidized sodium alginate, 0.1mg / ml methylene blue, with PBS as the buffer system, pH7.5.
Embodiment 2
[0053] The acquisition of embodiment 2 collagen biomaterials
[0054] 2.1 Sclera
[0055] New Zealand white rabbits were killed by air embolism, the eyeballs were taken out, and the sclera was separated. After being soaked in dodecyl dimethyl benzyl ammonium bromide solution, then placed in polyethylene glycol octyl phenyl ether solution for incubation, placed in trypsin and EDTA mixed solution for incubation, and placed Incubate in a mixed solution of DNase-1 and RNase-A to obtain decellularized sclera tissue. Slices were made into 1cm×1cm. Obtain the decellularized material.
[0056] 2.2 Vascular tissue
[0057] Fresh and suitable bovine veins were selected and decellularized by a multi-step detergent-enzyme digestion method. Soak in 0.25% trypsin solution for 18 hours, then place in 0.1% SDS for 12 hours, then immerse the tissue in 0.25% trypsin solution for 12 hours, and finally treat it in Dispase at 25°C for 12 hours, and finally place the decellularized cow neck ...
Embodiment 3
[0060] Embodiment 3 cross-linking reaction
[0061] The decellularized material slices obtained in Example 2 were respectively soaked in 20 mL of the solution of Example 1, and put into an 80 mL container. Maintain the temperature of the equilibrium system at 15°C, the pH at 7.5-7.8, and soak for 60 minutes. Subsequently, under the osmotic pressure of 310-330 Osm, the cross-linking reaction was carried out for 60 minutes under the condition of 1000w incandescent light irradiation and oxygen flow. Finally, with 0.25M Na 2 CO 3 / NaHCO 3 buffer solution to adjust the pH of the reaction system to 7, soak and shake for 1 hour, and seal and store for later use.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com