Method for increasing hydrogen production of HAU-M1 photosynthetic bacterium groups through fermentation of corn straw
A technology of HAU-M1 and corn stalk is applied in the field of microbial fermentation and biological hydrogen production, which can solve the problems of low hydrogen production rate and waste of resources, and achieve the effects of improving efficiency, clear steps and reasonable design.
Active Publication Date: 2020-04-03
HENAN AGRICULTURAL UNIVERSITY
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Problems solved by technology
Failure to control the metabolic pathway of photosynthetic hydrogen production will result in low hydrogen production rate and waste of resources
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[0042] 1.1 Test method
[0043] A method for improving HAU-M1 photosynthetic bacteria group to use corn stalks to ferment and produce hydrogen, the specific steps are as follows:
[0044] (1) Pretreatment of corn stalks: dry the corn stalks, crush them to 80-100 μm, and set aside;
[0045] (2) Pretreatment of the catalyst: crush the catalyst to 80-100μm, and then place the crushed catalyst in 1-1.5mol / L H 2 SO 4 Medium treatment for 24 hours, after cleaning, set aside;
[0046] (3) Photosynthetic hydrogen production of HAU-M1 photosynthetic bacteria group: 150 mL conical flask was used as the reactor for the experiment, and 5 g corn stalks, 1-7 mL cellulase (liquid enzyme preparation), 120 mL Citric acid-sodium citrate buffer (pH4.8) and hydrogen production medium, and use 5 mol / L NaOH to adjust the pH value to 7, and then add different qualities of activated carbon, diatomaceous earth or zeolite as catalysts, the amount added 0.5 g, 1 g, 1.5 g, 2 g, and 2.5 g, respectivel...
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The invention belongs to the technical field of biological hydrogen production through microorganism fermentation, and particularly relates to a method for increasing hydrogen production of HAU-M1 photosynthetic bacterium groups through fermentation of corn straw. According to the method disclosed by the invention, the corn straw degraded by cellulose is used as a hydrogen production substrate, and through adding a catalyst, efficient hydrogen production of the HAU-M1 photosynthetic bacterium groups can be promoted. According to the experiment, through adding activated carbon, diatomite and zeolite of different concentrations, a fermentation way in the process of photosynthetic organism hydrogen production of the corn straw is changed, the photosynthetic hydrogen production quantity is significantly increased, wherein the diatomite significantly increases the photosynthetic hydrogen production quantity of the corn straw, and the hydrogen production quantity is increased by 15.93% thanthat of the control group; and the zeolite significantly reduces the concentration of propanoic acid in reaction liquid and changes the light fermentation way of the corn straw, so that the hydrogen production quantity is increased by 33.33% than that of the control group. The method disclosed by the invention is reasonable in design, clear in steps, and simple and easy to operate, and the hydrogen production efficiency of the photosynthetic bacterium groups HAU-M1 through the corn straw can be improved.
Description
technical field [0001] The invention belongs to the technical field of microbial fermentation biological hydrogen production, and in particular relates to a method for improving HAU-M1 photosynthetic bacteria group to use corn stalks to ferment hydrogen production method. Background technique [0002] Hydrogen is a high calorific value, clean energy, it plays an important role in many fields. Compared with traditional industrial hydrogen production, biological hydrogen production has better prospects. It does not need to consume a large amount of non-renewable resources, does not produce a large amount of greenhouse gases, and can degrade agricultural biomass waste, food processing plant sewage, livestock Pollutants such as poultry manure. There are two main methods of biohydrogen production: dark fermentation and light fermentation. Compared with dark fermentation biological hydrogen production, light fermentation hydrogen production has many advantages such as stable hyd...
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Login to View More IPC IPC(8): C12P39/00C12P3/00C12R1/38C12R1/01
CPCC12P39/00C12P3/00Y02E50/30
Inventor 路朝阳张全国胡建军王毅荆艳艳贺超金鹏刘泽先张学婷岳田
Owner HENAN AGRICULTURAL UNIVERSITY