Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Biomarkers for Osteoporosis Diagnosis and Treatment

An osteoporosis and reagent technology, applied in the field of biomedicine, can solve the problem of unclear pathogenesis of osteoporosis, and achieve the effect of improving the quality of life

Active Publication Date: 2021-06-18
北京市创伤骨科研究所
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although current studies suggest that the onset of osteoporosis is related to age, gender, and is closely related to genetic factors and environmental factors, the pathogenesis of osteoporosis is still unclear. Early diagnosis, treatment monitoring and prognosis evaluation of osteoporosis Plays an important role in the prevention and treatment of osteoporosis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biomarkers for Osteoporosis Diagnosis and Treatment
  • Biomarkers for Osteoporosis Diagnosis and Treatment
  • Biomarkers for Osteoporosis Diagnosis and Treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Detection of the expression of LOC101927627 in osteoporosis by QPCR

[0044] 1. Sample collection

[0045] Selected 80 patients with hip replacement surgery, aged 50-75 years, including 47 patients with osteoporosis and 33 normal controls, and collected blood samples and tissue samples from the patients.

[0046] Inclusion and exclusion criteria for patients with osteoporosis:

[0047] Inclusion criteria: Patients diagnosed as needing hip replacement surgery; patients aged 50-75 years old and diagnosed as osteoporosis by dual-energy X-ray absorptiometry.

[0048] Exclusion criteria: The patient has taken drugs that affect bone metabolism within the past 6 months, and the patient also suffers from other diseases.

[0049] Inclusion and exclusion criteria for the control group:

[0050] Inclusion criteria: patients diagnosed as needing hip replacement surgery; patients aged 50-75 years old, and patients with non-osteoporosis determined by dual-energy X-ray ab...

Embodiment 2

[0086] Example 2 Expression of LOC101927627 in cells

[0087] 1. Isolation and culture of osteoblasts

[0088] Cancellous bone was taken from the human femoral head removed from hip replacement, the excess periosteum and soft tissue were removed, and the bone was repeatedly washed with normal saline. Rongol and scissors cut the femoral neck to 1mm 3 Fragments of osteoblasts were isolated and purified by enzymatic digestion.

[0089] The cell suspension obtained by enzymatic digestion was counted with a hemocytometer, and the concentration was diluted to 5×10 7 mL-1, inoculate it in a sterile petri dish, put in CO 2 Incubator (5% CO 2 , humidity 95%, temperature 37°C) for 48h, and the medium was changed every two days. The cells were nearly confluent and passaged (trypsin: 0.2% EDTA=1:2).

[0090]2. Extraction of total cellular RNA

[0091] The well-grown osteoblasts in log phase were taken for extraction of total cellular RNA, and cellular RNA was extracted by Trizol met...

Embodiment 3

[0096] Example 3 The effect of LOC101927627 on osteoblasts

[0097] 1. Design and synthesize siRNA against LOC101927627, siRNA-LOC101927627 and universal negative control siRNA-NC were purchased from Shanghai Jima Gene Chemical Technology Co., Ltd. The sequence of the interfering RNA against LOC101927627 is shown below.

[0098] Sequence of siRNA1:

[0099] The sense strand is 5'-UUUGAUCUUUAUCUAGAAGGC-3' (SEQ ID NO.5)

[0100] The antisense strand is 5'-CUUCUAGAUAAAGAUCAAAACC-3' (SEQ ID NO.6)

[0101] Sequence of siRNA2:

[0102] The sense strand is 5'-ACAACAUCAUUAUAACCAGAU-3' (SEQ ID NO.7)

[0103] The antisense strand is 5'-CUGGUUAUAAUGAUGUUGUGA-3' (SEQ ID NO.8)

[0104] 2. Cell culture

[0105] The osteocytes of Con group and OP group were cultured to logarithmic growth phase, and their concentration was measured and diluted. 5 Inoculated into a 6-well plate per well, and added the corresponding medium to make the final volume 2m1, put it at 37°C, 5% CO 2 Cultivated ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a biomarker for diagnosis and treatment of osteoporosis, and the biomarker is LOC101927627. The invention discloses the application of LOC101927627 in the preparation of osteoporosis diagnostic products, and the invention also discloses the application of LOC101927627 as a molecular target in the preparation of drugs for treating osteoporosis.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a biomarker for the diagnosis and treatment of osteoporosis, and the specific biomarker is LOC101927627. Background technique [0002] Osteoporosis is a systemic bone disease characterized by low bone mass and microarchitectural degradation in bone tissue, leading to increased bone fragility and increased risk of fracture. Osteoporotic fractures result in a significant decrease in quality of life and increased morbidity, mortality, and disability. [0003] According to the etiology of osteoporosis, it is divided into primary osteoporosis and secondary osteoporosis. Primary osteoporosis is associated with the aging process with a concomitant decrease in sex hormones. Bones have deteriorated in terms of microarchitecture, leading to a loss of bone mineral density and an increased risk of fractures. Secondary osteoporosis may be endocrine and metabolic disorders (eg, hypogonadism, hyperc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883A61K45/00A61P19/10
CPCA61K45/00A61P19/10C12Q1/6883C12Q2600/158C12Q2600/178
Inventor 吴成爱田伟王超王莹王倩倩张砚卓陶剑锋袁越
Owner 北京市创伤骨科研究所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com