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Scleroderma, application, fermentation medium and preparation method of PF1022A

A technology of PF1022A, fermentation medium, applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, can solve the problems of cumbersome steps of solid-phase synthesis method, unsuitable for large-scale production, complex process, etc., and shorten the fermentation time. The effect of cycle time, yield improvement and short fermentation time

Active Publication Date: 2020-04-07
CHENGDU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solid-phase synthesis method has cumbersome steps and complicated process, which is not suitable for large-scale production
However, there are relatively few reports on the production of PF1022A by microbial fermentation, and the yield is relatively low, which is limited to the laboratory level

Method used

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  • Scleroderma, application, fermentation medium and preparation method of PF1022A
  • Scleroderma, application, fermentation medium and preparation method of PF1022A

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Isolation and Characterization of Strain SIIA-16-1#

[0037] 1. Isolation of strain SIIA-16-1#:

[0038] The original strain SIIA-16-1# producing PF1022A was isolated and screened from the soil samples of Mengding Mountain in Ya'an, Sichuan.

test approach and

[0039] Test method and steps:

[0040] 1. Preparation of Media

[0041] (1) Formula: glucose 10.0g, peptone 5.0g, agar 20g, K2HPO4 1.0g, MgSO4·7 H2O 0.5g, 1 / 3000 Bengal red aqueous solution 100mL, 0.03℅ streptomycin dilution 100mL, tap water 800mL.

[0042] (2) Operation steps:

[0043] 1) Mix the raw materials according to the above recipe, and boil until the agar dissolves.

[0044] 2) Dispense it into 18mm×180mm test tubes while hot, 15mL per tube, stuff with tampons, label them, put them into small wire baskets, and wrap the tampons with old newspapers.

[0045] 3) Sterilize by high pressure steam at 121°C for 30 minutes.

[0046] Among them, the preparation process of 0.03% streptomycin solution is as follows: take 1g / bottle of domestic streptomycin, inhale 10mL of sterile water with a sterile pipette to dissolve, obtain 10% streptomycin solution, take 0.3mL of this solution to 100mL and sterilize A 0.03% streptomycin solution can be obtained in a bacterial volumetric...

Embodiment 2

[0079] 1. Isolation, mutagenesis and characteristics of the mutagenic strain SIIA-18-56#

[0080] 1. Isolation and mutagenesis of mutagenic strain SIIA-18-56#

[0081] 1.1 Bacterial suspension preparation:

[0082] The original strain SIIA-16-1# producing PF1022A was isolated and screened from the soil samples of Mengding Mountain in Ya'an, Sichuan. Add 10mL of sterile saline to the slant culture of strain SIIA-16-1# for washing, pour it into a shaker flask with crushed glass and vibrate to disperse, and make a bacterial suspension for later use.

[0083] 1.2 Compound mutagenesis treatment:

[0084] (1) Use a pipette gun to draw 10 μL of 1.1 medium bacterial suspension onto a glass slide with a diameter of 1 cm, and place it in a normal-pressure room-temperature plasma with helium as the working gas, a power of 120 W, a ventilation volume of 10 SLM, and a treatment distance of 2 mm. In vivo mutagenesis system (ARTP mutagenesis system). Treat 0s, 15s, 30s, 45s, 60s, and 80s...

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Abstract

The invention discloses a Rosellinia strain, its application, a fermentation medium and a preparation method of PF1022A. The Rosellinia strain has been preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 18132. The Rosellinia strain is used for production of PF1022A, and has advantages of fast mycelium growth, low mycelium concentration, short fermentation time and high fermentation unit.

Description

technical field [0001] The invention relates to the field of biotechnology, and in particular to a sclerocystis, its application, a fermentation medium and a preparation method of PF1022A. Background technique [0002] PF1022A is a kind of anthelmintic drug with low toxicity to animals, wide control spectrum and good effect. After macrolide anthelmintic drugs, it is the most potential class of anthelmintic drugs for livestock and pets. PF1022A can bind to the amino site of the hepta-helical latrophilin-like transmembrane receptor isolated from Haemonchus contortus Rudolphi, and its role is to induce external calcium influx into cells. The mode of action of this new anti-nematode drug is quite different from that of known anthelmintics, such as benzimidazoles, imidazothiazoles, and macrolides. Therefore, PF1022A insecticides are effective, and they also have a strong insecticidal effect on drug-resistant parasites. [0003] Currently PF1022A is synthesized by solid-phase sy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P17/14C12R1/645
CPCC12N1/14C12P17/14C12N1/145C12R2001/645
Inventor 王昆蓉俞岩青曾志刚田敏雷叶明马利明
Owner CHENGDU UNIV
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