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Arthrobacter for degrading chlorobenzene pollutants and culture method and application thereof

A cultivation method and technology of chlorobenzenes, applied in the field of Arthrobacter and its cultivation for degrading chlorobenzene pollutants, can solve the problems of lack of in-depth research, differences in optimal degradation efficiency, and different cultivation times, and achieve strong Substrate Adaptability, Effect of Good Substrate Broadness

Active Publication Date: 2020-04-07
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, through the analysis of the current research status of chlorobenzene degrading bacteria, it is found that there is no in-depth research on the chlorobenzene VOCs degrading bacteria screened based on the medical and chemical industry between high and low concentrations, and the culture time of the bacteria is also limited. There are no specific requirements, resulting in different incubation times and optimal degradation efficiencies.

Method used

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  • Arthrobacter for degrading chlorobenzene pollutants and culture method and application thereof
  • Arthrobacter for degrading chlorobenzene pollutants and culture method and application thereof
  • Arthrobacter for degrading chlorobenzene pollutants and culture method and application thereof

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Embodiment 1

[0041] Embodiment 1: Isolation and identification of bacterial strains:

[0042] 1. Domestication and breeding of strains

[0043] The activated sludge (adding liquid culture medium) taken from the sewage discharge outlet of the water company (March 2018, a water company in Zhengzhou, Zhongyuan Environmental Protection Co., Ltd.) was aerated and domesticated (2d), and then the enriched bacterial solution The centrifuged substrate (5mL), liquid medium and different concentrations of chlorobenzene organic substances were added to serum bottles, and cultured in a shaker for 4 days to obtain microbial communities that can degrade target pollutants (chlorobenzene VOCs); using solid culture Purification and separation of degradable strains by the base coating method, that is, take 100 μL of the two microbial liquids in the previously purified liquid medium, and spread them on the solid medium through the steps of the previous solid medium experimental method. Put it upside down in ...

Embodiment 2

[0059] Embodiment 2: Arthrobacter aureus (Paenarthrobacter ureafaciens LY) degradation characteristic

[0060] 1. Degradation characteristics of Arthrobacter under different pH conditions (5.6-8.4)

[0061] Take 10 mL of each of the above four nutrient solutions in 9 serum bottles, and dilute to 200 mL. Take 160 μL C 6 h 5Cl. Add 5mL of bacterial solution to the serum bottle of Paenarthrobacter ureafaciens LY. Use HCl (HCl 10mL plus distilled water 50mL) and NaOH (NaOH 10g plus distilled water 50mL) to adjust the pH values ​​of culture flasks 1, 2, 3, 4, and 5 to pH=5.3, pH=6.2, and pH=7.0, respectively , pH=8.0, pH=9.0, respectively placed at 30°C, 180r·min -1 Continuous cultivation in a constant temperature shaker, sampling and analysis at regular intervals (results such as Figure 4 ). The results showed that the optimal degradation pH of Paenarthrobacter ureafaciens LY was 7.0, and at C 6 h 5 In the gradual degradation process of Cl, its degradation characteristics...

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Abstract

The invention discloses arthrobacter for degrading chlorobenzene pollutants and a culture method and application thereof. The arthrobacter for degrading the chlorobenzene pollutants is named as Paenarthrobacter ureafaciens LY; the strain is preserved in of HMIGD (Herbarium Microbiology Institute of Guangdong); the preservation number is GDMCC NO.60676; and preservation date is May 15th, 2019. Thearthrobacter for degrading the chlorobenzene pollutants, which is provided by the invention, is an aerobic gram staining positive bacterium, grows by using monochlorobenzene for example as a carbon source and energy, and completely degrades the monochlorobenzene into CO2, H2O and inorganic chloride. The arthrobacter has the highest degradation efficiency for chlorobenzene VOCs (Volatile Organic Compounds) under the conditions of 30 DEG C and pH of 6.8 to 7.2, and the strain has excellent substrate adaptability and multi-substrate degradation capacity and reaches the maximum degradation efficiency of 90% for the monochlorobenzene. By research on biological characteristics and degradation characteristics of the strain, technical support can be provided for biological treatment on the chlorobenzene VOCs in the medical and chemical industries.

Description

technical field [0001] The invention relates to the field of microbial strains, in particular to an Arthrobacter for degrading chlorobenzene pollutants and a culture method and application thereof. Background technique [0002] The rapid growth of my country's economy has brought serious environmental problems, such as the pollution caused by the leakage and discharge of volatile organic pollutants (Volatile Organic Compounds, VOCs) in the production process of most industries, which seriously threatens human health. Research on the sources of VOCs in the atmospheric environment found that: the annual average contribution rate of man-made sources of VOCs emissions is 62% of automobile exhaust, 10% of liquefied petroleum gas, 9% of gasoline volatilization, 6% of paint, 6% of petrochemical industry, and unknown sources 6%. And VOCs exhaust gas generally has certain toxicity, which will cause harm to human body and plant environment. Studies have shown that about 70% of VOCs ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02B01D53/84B01D53/70C12R1/01
CPCC12N1/20C12N1/02B01D53/84B01D53/70B01D2251/95C12R2001/01C12N1/205Y02A50/20Y02P20/59
Inventor 刘楠马闯廉影陈亚辉段文辉刘从彬赵继红
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY