The invention discloses a silk cultural relic
fiber material identification method based on
amino acid analysis. The method comprises the following steps: (A) weighing 0.3-0.5mg of silk cultural relic sample in microgramme grade, putting the silk cultural relic sample in a
hydrolysis test tube, dissolving the silk cultural relic sample in 0.5-1.5ml of 6NHCl, vacuumizing while sealing a
test tube opening, hydrolyzing the sample for 22-24 hours at 110 DEG C; (B)
drying the hydrolyzed sample by using a
nitrogen blowing instrument, redissolving in 1.5-2.5ml of 10.1N HCl, freezing,
drying and preserving at minus 20 DEG C till the chromatographic analysis is implemented; (C) performing ammonic acid separation and detection by using an AQC
amino acid analysis packet and 2695HPLC of Waters company of America; (D)
processing data, representing the content of each
amino acid by utilizing relative mol percentage, when the mol ratio of the
glycine to
alanine to
serine is 4: 3: 1, deducing that the
fiber material of the silk culture relic is mulberry silk, when the mol ratio of the
glycine to
alanine to
serine is 3: 4: 1, deducing that the
fiber material of the silk culture relic is tussah silk. The variety and the content of the amino acid can be flexibly detected, and the culture relic cannot be greatly damaged since the sample quantity demand is few. The method disclosed by the invention is suitable for the identification of the silk cultural relic fiber material.