Near-infrared two-zone wide-field microscopy imaging system based on multi-dimensional adjustment frame

Abstract

The invention discloses a near-infrared two-zone fluorescence wide-field microscopic imaging system based on a multi-dimensional adjustment frame. The invention is based on a commercial upright fluorescence microscope module, and the epi-illuminator is installed on a multi-dimensional adjustment frame, which can realize multi-dimensional translation and rotation, so that the objective lens can be conveniently moved directly above the imaging plane. The excitation light is introduced from the rear port of the epi-illuminator, reflected by the dichroic mirror and irradiated on the sample through the objective lens. The fluorescence emitted by the sample is collected by the objective lens and then imaged on the detection surface of the InGaAs camera through the lens tube lens to realize fluorescence microscopy. imaging. The objective lens is installed on the electric focusing module, which can realize precise focusing and tomographic imaging. The invention overcomes the disadvantage that the original product cannot image larger experimental animals, can be precisely and conveniently adjusted vertically above the imaging window, is very suitable for wide-field microscopic imaging of large animals, has excellent performance, is convenient to use, and is suitable for popularization.

Description

technical field [0001] The invention belongs to the field of micro-imaging of applied optics, and relates to a near-infrared two-zone wide-field fluorescence micro-imaging system based on a multi-dimensional adjustment frame. Background technique [0002] Fluorescence signals emitted by organisms can reveal a wealth of information, and fluorescence-based biomicroscopic imaging has been widely used in life science research. Compared with traditional transmission microscopy imaging, fluorescence microscopy imaging has the advantages of high spatial resolution, high imaging signal-to-noise ratio, and specific labeling. Fluorescence microscopy imaging systems are generally composed of an illumination part, an imaging part, a beam splitter (such as a dichroic mirror), an objective lens, and a sample. Epi-illumination is often used to excite the sample to generate fluorescence, and the beam splitter is used to separate the excitation light and signal light. [0003] Today, there...

AI Technical Summary

Problems solved by technology

At the same time, because the autofluorescence of biological tissues is relatively small in this region, the fluorescence imaging of the second near-infrared region has a higher signal-to-background ratio; limited by the development of detectors and optical devices in this band, the imaging system of the second region of the near-infrared is still limited. , the imaging performance still needs to be improved

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