Method for simultaneously preparing rare ginsenoside and grease unsaponifiable matter
A technology for rare saponins and saponins of ginseng, which is applied in the directions of pharmaceutical formulations, plant raw materials, medical preparations containing active ingredients, etc., can solve the problems of wasting manpower and material resources, not seeing oil unsaponifiables and ginsenosides, and complicated operations.
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Embodiment 1
[0032] Process dried ginseng into powder, take 5g, add 50mL petroleum ether, 1.0g sodium hydroxide, 25mL n-butanol, 50mL ethanol, 50mL water, extract with a stirring tank or a stirrer, stirring shaft speed: 120rpm, temperature: 140 degrees centigrade, heating time is 0.5 hour, stand still for 90 minutes after heating, take the upper layer solution, reduce pressure or normal pressure to concentrate, make ginseng oil unsaponifiable matter. Take the solution in the lower layer, concentrate it to 1 / 4 of the original volume, add 2.5g of macroporous resin, the type of macroporous resin is D101, first elute with water until the ethanol eluent is colorless, clear and transparent, with a pH value of 7, Then elute with ethanol solution with a volume ratio of 40%, until the ethanol eluate is colorless, clear and transparent, collect the ethanol eluate, recover ethanol, and dry to obtain rare ginseng saponins in the form of dry extract.
[0033] The ginseng oil unsaponifiables prepared in...
Embodiment 2
[0051] Process dried ginseng into powder, take 5g, add 100mL petroleum ether, 1.5g sodium hydroxide, 50mL n-butanol, 80mL ethanol, 150mL water, extract with a stirring tank or a stirrer, stirring shaft speed: 140rpm, temperature: 120 degrees centigrade, heating time is 1.0 hour, stand still for 150 minutes after heating, take the upper layer solution, reduce pressure or normal pressure to concentrate, obtain ginseng oil and fat unsaponifiable matter. Take the solution in the lower layer, concentrate to 1 / 5 of the original volume, add 5.0 g of macroporous resin, the type of macroporous resin is D130, first elute with water until the ethanol eluent is colorless, clear and transparent, with a pH value of 7, Then elute with ethanol solution with a volume ratio of 50%, until the ethanol eluate is colorless, clear and transparent, collect the ethanol eluate, recover ethanol, and dry to obtain rare ginseng saponins in the form of dry extract.
Embodiment 3
[0053] Process the dried ginseng into powder, take 5g, add 150mL petroleum ether, 2.0g sodium hydroxide, 80mL n-butanol, 150mL ethanol, 250mL water, extract with a stirring tank or a stirrer, the stirring shaft speed: 160rpm, and the temperature is: 105 degrees centigrade, heating time is 1.5 hours, stand still for 120 minutes after heating, take the upper layer solution, reduce pressure or normal pressure to concentrate, make ginseng oil unsaponifiable matter. Take the lower layer solution, concentrate it to 1 / 6 of the original volume, put it in 6.0g macroporous resin, the macroporous resin model is AB8, first elute with water until the ethanol eluent is colorless, clear and transparent, and the pH value is 7, Then elute with ethanol solution with a volume ratio of 50%, until the ethanol eluate is colorless, clear and transparent, collect the ethanol eluate, recover ethanol, and dry to obtain rare ginseng saponins in the form of dry extract.
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