Extraction method of angelica sinensis ferulic acid

An extraction method, the technology of ferulic acid, applied in the field of extracting angelica ferulic acid, can solve the problems that the yield has not been greatly improved, the process requirements are strict, and the steps are complicated, so as to avoid nutrient loss, high extraction rate, The effect of low production cost

Inactive Publication Date: 2020-04-17
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Most of the extracts of angelica ferulic acid in the market use hot water extraction and ultrasonication, but the yields of these methods are low. There are also extraction methods of cellulase and pectinase in the market, but the process requirements are strict. , the steps are complicated, and the yield has not been greatly improved. The market needs a method with simple process steps and improved ferulic acid extraction rate. The present invention solves such problems

Method used

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  • Extraction method of angelica sinensis ferulic acid
  • Extraction method of angelica sinensis ferulic acid
  • Extraction method of angelica sinensis ferulic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The method of the present invention: select 5 g of fresh angelica roots and cut them to a length of 1-2 cm, place them in a refrigerator at -40°C, freeze them for 4 hours, take them out, put them in a water bath at 0°C to thaw, and repeat three times. Put the angelica thawed for the last time in a juice extractor, add 10 times the amount of 70% methanol, and cold press for 3 minutes. Put the squeezed angelica solution in a 50ml volumetric flask, wash the container with a small amount of 70% methanol several times, put the lotion into the volumetric flask together, add distilled 70% methanol to the mark, shake well, then suction filter, and discard the medicinal residues. Take 10mL of the filtrate and centrifuge at 8000r / min for 20min to obtain the primary extract of ferulic acid.

[0027] Detection: Use a syringe to measure 1-3mL of the supernatant, pass through a 0.22um filter membrane, use acetonitrile-phosphoric acid solution (17:83) as the mobile phase, detect in a ...

Embodiment 2

[0052] Example 2, freezing temperature is -10°C, thawing at 0°C;

[0053]Choose 30g of fresh angelica roots and cut them to 2-3cm long, put them in a -10°C refrigerator, freeze for 4 hours, take them out, put them in a 0°C water bath to thaw, and repeat three times. Put the angelica thawed for the last time in a juice extractor, add 10 times the amount of 70% methanol, and cold press for 3 minutes. Put the squeezed angelica solution in a 500ml volumetric flask, wash the container with a small amount of 70% methanol several times, put the lotion into the volumetric flask together, add 70% methanol to the mark, shake well, then suction filter, and discard the medicinal residues. Take 50mL of the filtrate and centrifuge at 8000r / min for 10min, and take the supernatant to obtain the initial ferulic acid extract.

[0054] Detection: Use a syringe to measure 1-3mL of the supernatant, pass through a 0.22um filter membrane, use acetonitrile-phosphoric acid solution (17:83) as the mob...

Embodiment 3

[0058] Example 3, the freezing temperature is -20°C and thawed at 0°C;

[0059] Select 30g of fresh angelica roots and cut them to 2-3cm long, put them in a -20°C refrigerator, freeze them for 4 hours, take them out, put them in a 0°C water bath to thaw, and repeat three times. Put the angelica thawed for the last time in a juice extractor, add 10 times the amount of 70% methanol, and cold press for 3 minutes. Put the squeezed angelica solution in a 50ml volumetric flask, wash the container with a small amount of 70% methanol several times, put the lotion into the volumetric flask together, add 70% methanol to the mark, shake well, then suction filter, and discard the medicinal residues. Take 10ml of the filtrate and centrifuge at 8000r / min for 20min to obtain the primary extract of ferulic acid.

[0060] Detection: Use a syringe to measure 1-3mL of the supernatant, pass through a 0.22um filter membrane, use acetonitrile-phosphoric acid solution (17:83) as the mobile phase, d...

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Abstract

The invention discloses an extraction method of angelica sinensis ferulic acid. The extraction method comprises the following steps: 1, pretreating fresh angelica sinensis tuberous roots; 2, freezingthe pretreated angelica sinensis for 4-8 h at -20 to -80 DEG C, then unfreezing the angelica sinensis in a water bath at 0-40 DEG C for 1-3 times, putting the tubers unfrozen for the last time into ajuice extractor, adding a solvent into the juice extractor, performing cold pressing, uniformly shaking, filtering, and discarding medicine residues to obtain a filtrate; 3, centrifuging the filtrate,and taking the obtained supernatant to obtain a test solution. The ferulic acid extraction rate is greatly increased by using a freeze-thawing method, the extraction condition most suitable for extracting the angelica sinensis ferulic acid is found through optimization experiments to further increase the extraction rate, and the nutrition and effective components of the raw materials are reserved.

Description

technical field [0001] The invention relates to an extraction process, in particular to an extraction method of angelica ferulic acid. Background technique [0002] Angelica is sweet, pungent and warm in nature. Return liver, heart, spleen channel. It has the effects of nourishing blood and promoting blood circulation, regulating menstruation and relieving pain, moistening intestines and laxative. "Compendium of Materia Medica" records that angelica treats headaches, all kinds of pain in trusted subordinates, moisturizes the intestines, stomach, muscles, bones and skin, treats carbuncles, drains pus and relieves pain, and nourishes blood. Modern pharmacological studies have shown that angelica can reduce myocardial excitability; dilate coronary arteries and increase coronary flow; reduce myocardial oxygen consumption and blood pressure; have anti-arrhythmic effects, anti-atherosclerotic effects, and improve microcirculation. The chemical composition of angelica is mainly ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C51/42C07C59/64
CPCC07C51/42C07C59/64
Inventor 梁宗锁崔倩倩郭建军胡万哉陈喜良
Owner ZHEJIANG SCI-TECH UNIV
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