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Long-acting quality-guaranteeing composite microbial agent and preparation method thereof

A compound microbial agent and long-acting technology, which is applied in aquaculture, microbial agent, ecological planting, and agricultural microbiology, can solve the problems of short storage period, inconspicuous fertilizer effect, single strain of microbial agent, etc. Oxygen, the effect of reducing the amount of chemical fertilizers

Active Publication Date: 2020-04-24
鸿坤土壤修复科技(苏州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a long-term quality-guaranteed compound microbial liquid bacterial agent and its preparation method, which solves the problems of single microbial strain, short storage period and inconspicuous fertilizer effect

Method used

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  • Long-acting quality-guaranteeing composite microbial agent and preparation method thereof
  • Long-acting quality-guaranteeing composite microbial agent and preparation method thereof
  • Long-acting quality-guaranteeing composite microbial agent and preparation method thereof

Examples

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preparation example Construction

[0040] A preparation method for a long-term quality-preserving compound microbial bacterial agent, comprising the following steps:

[0041] 1. 4 kinds of aerobic bacteria Bacillus colloidus, Streptomyces flavinus, Bacillus subtilis and Bacillus amyloliquefaciens were respectively cultivated to make 4 kinds of liquid aerobic bacteria agents,

[0042] (1) Prepare the liquid aerobic culture medium of Bacillus colloidus, Streptomyces flavinus, Bacillus subtilis and Bacillus amyloliquefaciens respectively, and then inoculate respective corresponding strains in 4 kinds of liquid aerobic culture medium respectively, Under the conditions of 33-37°C, 100-140r / min, and pH value of 6.0-7.5, the primary expansion culture of the strains was carried out to obtain 5 kinds of primary aerobic bacterial liquids;

[0043] (2) Take 4 kinds of first-level aerobic bacteria liquids and inoculate the respective corresponding strains into the 4 kinds of liquid aerobic culture mediums, and carry out th...

Embodiment 1

[0061] Composite microbial bacterial agent comprises 4 kinds of liquid aerobic bacterial agents cultivated with liquid medium and 4 kinds of liquid anaerobic bacterial agents cultivated with liquid medium, and the mass ratio of liquid aerobic bacterial agent and liquid anaerobic bacterial agent is 2: 3.

[0062] Liquid aerobic bacterial agent is made up of the bacterial agent of following mass parts:

[0063] 2 parts by mass of the liquid Streptomyces luteus agent; 3 parts by mass of the liquid Bacillus subtilis agent; 2 parts by mass of the liquid Bacillus amyloliquefaciens agent; 3 parts by mass of the liquid Bacillus subtilis agent.

[0064] Liquid anaerobic bacterial agent is made up of the bacterial agent of following mass parts:

[0065] 3 parts by mass of liquid photosynthetic bacteria; 2 parts by mass of liquid Saccharomyces cerevisiae inoculum; 5 parts by mass of liquid plant milk inoculum; 5 parts by mass of liquid Lactobacillus acidophilus inoculum.

[0066] Where...

Embodiment 2

[0089] Composite microbial bacterial agent comprises 4 kinds of liquid aerobic bacterial agents cultivated with liquid medium and 4 kinds of liquid anaerobic bacterial agents cultivated with liquid medium, and the mass ratio of liquid aerobic bacterial agent and liquid anaerobic bacterial agent is 10: 11.

[0090] Liquid aerobic bacterial agent is made up of the bacterial agent of following mass parts:

[0091] 1 part by mass of liquid Streptomyces flavinus agent; 3 parts by mass of liquid Bacillus subtilis agent; 3 parts of liquid Bacillus amyloliquefaciens; 3 parts by mass of liquid Bacillus subtilis agent.

[0092] Liquid anaerobic bacterial agent is made up of the bacterial agent of following mass parts:

[0093] 2 parts by mass of liquid photosynthetic bacteria; 3 parts by mass of liquid Saccharomyces cerevisiae inoculant; 3 parts by mass of liquid plant milk inoculum; 3 parts by mass of liquid Lactobacillus acidophilus agent.

[0094] Wherein, each kind of liquid aerob...

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Abstract

The invention relates to the technical field of agricultural microorganisms, ecological planting, aquaculture and microbial agents, and especially relates to a long-acting quality-guaranteeing compound microbial agent and a preparation method thereof. The composite microbial agent comprises a liquid aerobic microbial agent and a liquid anaerobic microbial agent, and the liquid aerobic microbial agent comprises a liquid Streptomyces microflavus agent, a liquid Bacillus subtilis agent, a liquid Bacillus amyloliquefaciens agent and a liquid Bacillus mucilaginosus agent; and the liquid anaerobic bacteria agent comprises liquid photosynthetic bacteria, a liquid Saccharomyces cerevisiae agent, a liquid Lactobacillus plantarum agent and a liquid Lactobacillus acidophilus agent. The composite microbial liquid agent and the preparation method thereof solve the problems of single strain, short storage life and unobvious fertilizer efficiency of microbial agents.

Description

technical field [0001] The invention relates to the technical fields of agricultural microorganisms, ecological planting, aquaculture and microbial inoculum, in particular to a long-term quality-guaranteed composite microbial inoculum and a preparation method thereof. Background technique [0002] At present, the abuse of chemical fertilizers in my country's planting and aquaculture industries is very common. The excessive use of chemical fertilizers has caused harmful substances such as nitrite, ammonia nitrogen, and hydrogen sulfide in soil and water to exceed the standard, resulting in a decline in the quality of agricultural products and threatening human health. It also caused serious damage to the ecological environment. [0003] In addition, the unreasonable application of chemical fertilizers leads to the continuous decline of soil conditions, not because of insufficient soil fertility, but because too many nutrients are consolidated in the soil and cannot be absorbed...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18C09K17/14C09K17/42C12R1/465C12R1/125C12R1/07C12R1/865C12R1/25C09K101/00
CPCC12N1/20C12N1/18C09K17/14C09K17/42C09K2101/00
Inventor 陈仲玉
Owner 鸿坤土壤修复科技(苏州)有限公司
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