Fat extract, fat acellular matrix and preparation method and application of fat acellular matrix
A technology of acellular matrix and extraction liquid, applied in the field of fat acellular matrix and its preparation, and fat extraction liquid, can solve the problems of increasing operation difficulty and biological pollution, and achieve the advantages of shortening processing time, avoiding damage and high biological safety. Effect
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[0043] Example 1: Preparation of Adipose liquid extract (ALE) and M-DAT (Mechanical processing DAT: DAT prepared by physical method)
[0044] 1) Wash the obtained granular fat with Phosphate buffered saline (PBS), centrifuge at 1000g / min for 3min, discard the upper fat and bottom liquid after centrifugation, and collect the middle fat tissue.
[0045] 2) Mix the adipose tissue obtained in step 1) with an equal volume of PBS, and transfer to a 10 ml screw-top syringe.
[0046] 3) Connect the fat-containing syringe obtained in step 2) and a new 10ml screw-top syringe through a 2.4mm luer connector, and push the plunger of the fat-containing syringe to push the fat into the opposite syringe. The bolus injection speed is 10ml / s, so continuous round-trip bolus injection for 1 min.
[0047] 4) Centrifuge the bolus product obtained in step 3) at 2000g / min for 5min, discard the upper layer of oil, and collect the bottom liquid part to be the Adipose liquid extract (ALE) and store it at -20°C ...
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[0055] Example 2: Preparation of fat extract ALE and fat acellular matrix M-DAT
[0056] 1) Wash the obtained granular fat with Phosphate buffered saline (PBS), centrifuge at 800g / min for 2min, discard the upper fat and bottom liquid after centrifugation, and collect the middle fat tissue.
[0057] 2) Mix the adipose tissue obtained in step 1) with an equal volume of PBS, and transfer to a 10 ml screw-top syringe.
[0058] 3) Connect the fat-containing syringe obtained in step 2) and a new 10ml screw-top syringe through a 1.4mm luer connector, and push the plunger of the fat-containing syringe to push the fat into the opposite syringe. The bolus injection speed is 8ml / s, so the continuous round-trip bolus injection is 0.5min.
[0059] 4) Centrifuge the bolus product obtained in step 3) at 1500g / min for 3min, discard the upper layer of grease, collect the bottom liquid part, which is ALE and store it at -20°C for later use, and boluse the deoiled fat from the middle layer The tissue w...
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