High performance liquid chromatography analysis method of traditional Chinese medicine prescription Dachengqi decoction

A technology of high-performance liquid chromatography and Dachengqi Decoction, which is applied in the field of high-performance liquid chromatography analysis of the traditional Chinese medicine prescription Dachengqi Decoction, can solve problems that have not yet been discovered, and achieve the effect of wide applicability and convenient operation

Pending Publication Date: 2020-04-28
GUANGXI INT ZHUANG MEDICINE HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, no method has been found to simultaneously determine naringin, hesperidin, neohesperidin, aloe-emodin, rhein, honokiol, magnolol, and emodin in Dachengqi Decoction composed of different processed rhubarb. , chrysophanol, emodin methyl ether and other 10 kinds of reports

Method used

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  • High performance liquid chromatography analysis method of traditional Chinese medicine prescription Dachengqi decoction
  • High performance liquid chromatography analysis method of traditional Chinese medicine prescription Dachengqi decoction
  • High performance liquid chromatography analysis method of traditional Chinese medicine prescription Dachengqi decoction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Simultaneous determination of the contents of 10 active ingredients in Dachengqi Decoction, a different processed product of rhubarb

[0042] 1. Experimental materials

[0043] 1.1 Instrument

[0044] Model 2695 high-performance liquid chromatography, including 2489UV, quaternary pump, vacuum degassing pump, automatic sampler, column thermostat and Empower3 liquid phase workstation (Waters, USA); KQ5200B ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd. ); electronic analytical balance (Shanghai Shensheng Technology Co., Ltd., D1810C); DHG-9203A electric heating constant temperature blast drying oven (Shanghai Qixin Scientific Instrument Co., Ltd.); TGL-16G high-speed desktop centrifuge (Shanghai Anting Scientific Instrument Factory );

[0045] Simplicity pure water system; (France, Millipore); HWS-26 electric heating constant temperature water bath (Shanghai Qixin Scientific Instrument Co., Ltd.); WND-200 universal pulverizer (Tianjin Taiste Instr...

Embodiment 2

[0074] Embodiment 2 Negative control experiment

[0075] Samples were prepared according to the method under "Item 2.2" of Example 1, and the negative samples of Dachengqi Decoction that did not contain rhubarb processed products, Magnolia officinalis, Fructus Citrus Fructus Citrus Fructus Citrifolia and Glauber's Salt were prepared respectively, and then prepared according to the method under "Item 2.3" of Example 1 The negative control solution of the test product was centrifuged, filtered through a 0.45 μm microporous membrane and stored for later use. Inject 10 μl of the test solution, the mixed reference solution and the negative control solution respectively, and measure its content, naringin, hesperidin, neohesperidin, aloe-emodin, rhein, honokiol, magnolol , emodin, chrysophanol and emodin methyl ether retention time are about: 42.7, 44.1, 45.9, 62.3, 64.9, 72.1, 73.5, 79.1, 83.7, 90.8min. After comparison of the experimental chromatograms, it was shown that the negat...

Embodiment 3

[0076] The investigation of embodiment 3 chromatographic conditions

[0077] 1. Selection of mobile phase

[0078] Investigation and comparison of methanol-water, methanol-0.1% glacial acetic acid, methanol-0.3% glacial acetic acid, methanol-0.2% phosphoric acid, methanol-0.1% phosphoric acid, methanol-0.05% phosphoric acid, acetonitrile-water, acetonitrile-0.1% phosphoric acid and acetonitrile The influence of -0.2% phosphoric acid and other elution systems on peak elution time, resolution and baseline. It was found that the use of acetonitrile-0.1% phosphoric acid system has better separation of chromatographic peaks and a more stable baseline. Appropriately adjust the gradient elution The procedure can achieve good separation of each peak, and the baseline is relatively balanced, so the acetonitrile-0.1% phosphoric acid system is used for gradient elution.

[0079] 2. Selection of Elution Conditions

[0080]Investigate the effects of different column temperatures and flow...

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Abstract

The invention relates to the field of medicine analysis, in particular to a high performance liquid chromatography analysis method of a traditional Chinese medicine prescription Dachengqi decoction. The method comprises the following steps: (1), making preparation of a test sample; to be specific, precisely taking Dachengqi decoction, diluting the decoction with absolute ethyl alcohol, fixing thevolume, carrying out uniform shaking and centrifuging, taking supernatant, carrying out filtering, and taking subsequent filtrate to obtain a test sample solution; and (2), carrying out high performance liquid chromatography determination; to be specific, precisely absorbing the test sample solution, and injecting the test sample solution into a high performance liquid chromatograph for determination. The method disclosed by the invention can be used for simultaneously determining 10 main medicinal components in the Dachengqi decoction and is convenient to operate; and a basis is provided forestablishing a fingerprint spectrum. The method is suitable for determining the effective components of the Dachengqi decoction prepared from different processed rheum officinale products and has a wide application range.

Description

technical field [0001] The invention relates to the field of drug analysis, and relates to a high-performance liquid chromatography analysis method for a traditional Chinese medicine prescription Dachengqi Decoction. Background technique [0002] Dachengqi Decoction comes from Zhang Zhongjing's "Treatise on Febrile Diseases" in the Han Dynasty. It is composed of rhubarb, Citrus aurantium, Magnolia officinalis and Glauber's salt. Flow and Lire empirical evidence, etc. The main active ingredients of rhubarb are anthraquinone compounds, which exist in two forms: free form and bound form. Rhubarb anthraquinones are one of the purgative active ingredients of traditional Chinese medicine rhubarb, magnolols in magnolia bark have anti-pathogenic microorganisms and adjust gastrointestinal motility, etc. Pharmacological effects such as anti-inflammatory and diuretic. The above active ingredients are the main material basis for the pharmacological effects of Dachengqi Decoction, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/88
CPCG01N30/02G01N30/88G01N2030/047G01N2030/8809
Inventor 魏江存王成龙秦祖杰黄国东陈勇彭宪郭雨西梁迪黄英红施显怡马艳
Owner GUANGXI INT ZHUANG MEDICINE HOSPITAL
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