Preparation method of polyquaternium magnetic microsphere with core-shell structure

A technology of magnetic microspheres and polyquaternary ammonium salts, which can be applied in the preparation of microspheres, microcapsule preparations, chemical instruments and methods, etc., can solve the problems of low nucleic acid amount, limited saturation magnetization, low saturation magnetization, etc. The effect of strong grasping ability, increased production capacity and simple process flow

Inactive Publication Date: 2020-05-08
SUZHOU WEIDU BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, the saturation magnetization of some magnetic microspheres is low. Although the binding capacity is high, it is limited by the saturation magnetization of the magnetic microspheres, resulting in a low amount of isolated nucleic acid.

Method used

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  • Preparation method of polyquaternium magnetic microsphere with core-shell structure
  • Preparation method of polyquaternium magnetic microsphere with core-shell structure
  • Preparation method of polyquaternium magnetic microsphere with core-shell structure

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preparation example Construction

[0028] The invention provides a method for preparing magnetic microspheres with high saturation magnetization, high binding efficiency, simple process flow, and applicable to automation, capable of rapid biological macromolecules. The initiator, the crosslinking agent and the quaternary ammonium salt monomer are coated with the quaternary ammonium salt group on the surface of the seed microsphere through free radical polymerization.

[0029] The structural formula of the quaternary ammonium salt monomer is:

[0030]

[0031] In the formula, R1 has a carbon-carbon double bond, R2, R3, and R4 are the same or different hydrocarbon groups with a carbon chain length of 1 to 10 atoms arranged in a straight or branched chain, and R5 is a halogenated element, sulfonate or Sulfate A negatively charged group.

[0032] First, disperse the seed microspheres with a particle size of 10 nm to 5 μm in an organic solvent, and add a silane coupling agent under the condition of mechanical st...

Embodiment 1

[0039] Weigh 10g of ferroferric oxide magnetic microspheres (average particle diameter 100nm) and disperse them in the ethanol solution of 200ml, add 1g of gamma-methacryloxypropyltrimethoxysilane while stirring, then add 10g of Sodium hydroxide solution (0.5g / ml) was reacted at 40°C for 15 hours to obtain magnetic microspheres modified with double bonds on the surface, which were stored for future use.

Embodiment 2

[0041] Weigh 10g of ferroferric oxide magnetic microspheres (average particle diameter 500nm) and disperse them in the ethanol solution of 200ml, add 1g of gamma-methacryloxypropyltrimethoxysilane while stirring, then add 10g of Sodium hydroxide solution (0.5g / ml) was reacted at 70°C for 3 hours to obtain magnetic microspheres modified with double bonds on the surface, which were stored for future use.

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Abstract

The invention relates to a preparation method of polyquaternium magnetic microspheres with a core-shell structure, and the method comprises the following steps: dispersing seed microspheres in an organic solvent, adding a silane coupling agent under the condition of mechanical stirring, regulating the pH value to make the solution alkaline, and reacting to obtain double-bond-modified magnetic microspheres; dispersing the double-bond modified magnetic microspheres into an organic solvent, then adding a quaternary ammonium salt monomer, an initiator and a cross-linking agent, heating to 40-90 DEG C in a water bath, and reacting for 1-24 hours to obtain the quaternary ammonium salt modified magnetic microspheres. The quaternary ammonium salt has very strong adsorption capacity on biomacromolecules, and can be used for quickly separating the biomacromolecules, and the yield is more than 90%; the surface of the polyquaternium magnetic microspheres is rich in quaternary ammonium salt, so that the polyquaternium magnetic microspheres have strong adsorption capacity to biomacromolecules and are high in separation yield. The prepared microspheres are especially suitable for automatic separation and extraction of the biomacromolecules.

Description

technical field [0001] The invention relates to a method for preparing polyquaternary ammonium salt magnetic microspheres with a core-shell structure, and belongs to the technical field of preparation of functional polymer microspheres. Background technique [0002] In recent years, magnetic microspheres, as a new type of bioseparation technology and solid-phase carrier for targeted drugs, have received widespread attention and research. From the early 1980s to today, there have been many products such as Dynabeads, SeraMag, Sepharose 4FF, Sepharose6FF, etc. The emergence of these magnetic microspheres breaks the traditional notion that organics and metals do not intersect. Magnetic microspheres have shown various excellent performances in biological separation: simple operation, high separation purity, etc., using these characteristics to simplify tedious and complicated operations and greatly shorten the test cycle. Therefore, magnetic microspheres have been widely used ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F292/00C08F220/34C08F212/36C08F222/38C08F226/02B01J13/02B01J20/26B01J20/28B01J20/30
CPCB01J13/02B01J20/264B01J20/28009B01J20/28021C08F292/00C08F220/34C08F226/02
Inventor 康英杰杜德壮杨永喆
Owner SUZHOU WEIDU BIOTECH CO LTD
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