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Preparation method of codfish skin collagen peptide

A technology of skin collagen and protein peptide is applied in the field of preparation of cod skin collagen peptide, which can solve the problems of low extraction rate of collagen peptide and the like

Inactive Publication Date: 2020-05-08
CHANGCHUN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the extraction rate of collagen peptides in these patents is relatively low

Method used

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  • Preparation method of codfish skin collagen peptide
  • Preparation method of codfish skin collagen peptide

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preparation example Construction

[0037] The reagents or instruments used in the preparation method of the cod skin collagen peptide provided by the present invention can be purchased from the market.

[0038] Materials and Reagents:

[0039] Materials: Cod skin was purchased from Weifang Beihai Refrigeration Co., Ltd.; experimental mice (48 5-week-old female mice, weighing 19-22 g) were purchased from Jinan Pengyue Experimental Animal Breeding Co., Ltd.

[0040] Reagents: activated carbon (CAS No. 64356-11-3), glycerol (CAS No. 56-81-5), chitotriose (CAS No. 41708-93-4), kestetose (CAS No. 13133-07- 8) and trypsin (enzyme activity: 200U / mg) were purchased from Cloud Reagent Mall - Science Grass Officer.

[0041] equipment:

[0042] Electric heating stainless steel reaction kettle 50L (Shandong Longxing Chemical Machinery Group); digital display three-purpose electric heating constant temperature water tank water bath (Jincheng Zhijie Experimental Instrument Factory, Jintan District); stainless steel constan...

Embodiment 1

[0044] Example 1 Cod Skin Collagen Peptide Preparation

[0045] The extraction process for preparing cod skin collagen peptides: cod skin—deashing treatment—hot water extraction—enzymatic hydrolysis—inactivation—decolorization, deodorization—membrane filtration—ion exchange—concentration—drying, the specific steps are as follows:

[0046] 1. Weigh 100g of cod skin for hot water extraction to obtain collagen; the parameters are: material ratio 1:4, extraction temperature 95°C, extraction time 2h;

[0047] 2. Cod skin collagen peptides were prepared by hydrolysis with trypsin, the amount of enzyme added was 4.5%, and the enzymatic hydrolysis time was 2 hours. In order to improve and protect the activity of trypsin, add enzyme protection agent (0.2% glycerol, 0.2% chitotriose, 0.2% kestose) to continue the reaction, heat at 98°C for 10min to inactivate the protein after the reaction, and centrifuge at 4000rpm 20min to get the supernatant.

[0048] 3. Use 2% activated carbon to ...

Embodiment 2

[0053] Example 2 Cod Skin Collagen Peptide Preparation

[0054] The amount of enzyme added is 5%, and other process parameters are the same as in Example 1.

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Abstract

The invention relates to the technical field of collagen extraction, in particular to a preparation method of codfish skin collagen peptide. The method comprises the following steps: mixing codfish skin with water, and performing extraction at 90-95 DEG C for 2-2.5 hours to obtain a mixed material; adding trypsin for enzymolysis, wherein the mass percent of the added trypsin is 4%-5.5%, and the enzymolysis time is 2-3 h; performing inactivating and centrifuging to obtain a supernatant; performing decolorizing and deodorizing with activated carbon, and performing filtration with a plate frame to obtain a decolorized solution; and performing filtration by an ultrafiltration membrane, and then carrying out ion exchange, concentration and freeze drying to obtain the cod skin collagen peptide.According to the method disclosed by the invention, the extraction rate of the collagen peptide can reach 94%-95%. The molecular weight is mainly below 1000Da, and the content is as high as more than80%. The collagen peptide is mainly composed of a polypeptide mixture containing 2-10 amino acids, and can remarkably increase the content of hydroxyproline and collagen in the skin of a model mouse,enhance the antioxidant enzyme activity in human blood and achieve the effect of delaying senescence.

Description

technical field [0001] The invention relates to the technical field of collagen extraction, in particular to a preparation method of cod skin collagen peptide. Background technique [0002] Collagen is a fibrous protein widely distributed in animal skin, hair, bone and blood vessels, which can support and protect the body. Collagen peptide is a polypeptide mixture with a molecular weight less than 3kDa produced by enzymatic hydrolysis of collagen. Compared with collagen, it has a better absorption rate; it also has properties that collagen does not have, such as anti-oxidation and inhibition of platelet aggregation. Because of its remarkable physiological activity, it is widely used in various fields such as functional food, health food and cosmetics. [0003] According to statistics, the annual processing volume of cod in my country has exceeded 500,000 tons, of which cod skin, as a by-product, is often discarded, causing environmental pollution and waste of resources. St...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/18C07K1/14
CPCC07K14/78C12P21/06
Inventor 赵珺胡彦波王西丁修庆刘佳鑫吴琼陈丽娜张莉弘
Owner CHANGCHUN UNIV
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