Uric acid electrochemical sensor and application thereof
An electrochemical and sensor technology, applied in the field of electrochemical analysis, can solve the problems of poor anti-interference ability of spectroscopy, complicated operation of chromatography, high cost of enzymatic method, etc., and achieve high catalytic site activity, high conductivity, and high sensitivity Effect
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Embodiment 1
[0048] Example 1, an electrochemical sensor for uric acid based on metal-organic framework-derived materials and its preparation method
[0049] The electrochemical sensor for uric acid based on metal-organic framework-derived materials is a three-electrode system;
[0050] The three-electrode system consists of an electrochemically active material-modified glassy carbon electrode as the working electrode, a saturated calomel electrode as the reference electrode, and platinum as the counter electrode; wherein, the electrochemically active material-modified glassy carbon electrode includes a glassy carbon electrode and a glassy carbon electrode. The encapsulated electrochemically active material is composed of gold nanoparticles and cobalt nanoparticles embedded in a hollow nanoporous carbon framework. The electrochemically active material-modified glassy carbon electrode is prepared by dispersing the electrochemically active material with N-methylpyrrolidone containing polyvin...
Embodiment 2
[0066] The preparation method of the uric acid electrochemical sensor based on the metal-organic framework derivative material is the same as the embodiment 1 of the present invention, the difference is that the chloroauric acid solution is added so that the concentration of trivalent gold ions in the methanol solution is 0.2 mg·mL -1 , etch metal-organic framework templates, fully and continuously stirred for 4 hours, programmed temperature rise, and the heating rate was 1°C min -1 , the temperature was raised to 800°C, and the temperature was kept at 8 hours. The electrochemically active material was dispersed into the N-methylpyrrolidone solution containing polyvinylidene fluoride. The mass ratio of the electrochemically active material to polyvinylidene fluoride was 5:1, and the electrochemical activity The content of the material in N-methylpyrrolidone solution is 2mg·mL -1 .
[0067] Configuration 25, 50, 100, 200, 400, 800, 1600μmol L -1 7 groups of uric acid standar...
Embodiment 3
[0069] The preparation method of the uric acid electrochemical sensor based on the metal-organic framework derivative structure is the same as that in Example 1 of the present invention. The difference is that the mass ratio of the electrochemically active material to polyvinylidene fluoride is 20:1, and the content of the electrochemically active material in the N-methylpyrrolidone solution is 4 mg·mL -1 .
[0070]Using the uric acid electrochemical sensor prepared above, measure according to the electrochemical determination method for determining uric acid content in Example 1 of the present invention, as follows: 10 groups of adult blood samples were obtained from the rheumatology department of a hospital in Shandong, centrifuged at 8000g and separated for 15 minutes to obtain For serum, human serum samples were diluted 100 times with phosphate buffer as the supporting electrolyte, the electrochemical signal of uric acid was obtained by differential pulse voltammetry, and ...
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