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Ultrahigh-flux platform for screening crystal generation conditions and screening method

A conditional and high-throughput technology, applied in the field of microfluidic screening, can solve the problems of time-consuming, low throughput, low efficiency and throughput, and achieve the effects of low reagent consumption, large screening range and fast experimental speed

Pending Publication Date: 2020-05-12
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional screening methods not only have the disadvantages of low throughput, high cost, and time-consuming, but also when there are many screening components (three components or more), the throughput is difficult to meet the screening requirements; and, as people are Different fields (such as pharmaceuticals, inorganic / organic substances, etc.) have higher and higher requirements on the properties of crystals, and the conditions for crystal formation are becoming more and more complex. Screening methods with high cost, low efficiency and low throughput are more difficult to meet the needs

Method used

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  • Ultrahigh-flux platform for screening crystal generation conditions and screening method
  • Ultrahigh-flux platform for screening crystal generation conditions and screening method
  • Ultrahigh-flux platform for screening crystal generation conditions and screening method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] The crystallization condition screening of drug crystals-indomethacin drug crystals, the specific steps are as follows:

[0055] S1. Microfluidic droplet ultra-high-throughput droplet generation experiment

[0056] S11. Fabrication of the microfluidic droplet generation chip: according to the co-flow focusing microchannel structure of the microfluidic droplet chip, the microfluidic droplet chip is fabricated, and the chip manufacturing method is selected from the molding method;

[0057] Preparation of microfluidic droplet chip: using PDMS (polydimethylsiloxane) and aluminum sheet as materials;

[0058] (1) Use SolidWorks2017 software to design the co-flow focusing microchannel structure generated by droplets, and then use a high-resolution and high-power laser engraving machine to engrave the channel structure on the aluminum sheet, and control the engraving depth of the channel by grasping the engraving time. Polishing treatment reduces the surface roughness of the a...

Embodiment 2

[0077] The crystallization condition screening of inorganic crystals-calcium carbonate crystals, the specific steps are as follows:

[0078] S1. Microfluidic droplet ultra-high-throughput droplet generation experiment

[0079] S11. Fabrication of the microfluidic droplet generation chip: the fabrication method is the same as that of S11 in Example 1.

[0080] S12, Preparation of 0.5 mol / L and 0.05 mol / L calcium chloride solution: Weigh 5.55 g of calcium chloride powder, place it in a 100 mL volumetric flask, add water to make it 100 ml, and place it in an ultrasonic cleaner Sonicate for 10 min to dissolve calcium chloride completely, and prepare 0.5 mol / L calcium chloride solution. Weigh 0.555 g of calcium chloride powder, place it in a 100 mL volumetric flask, add water to make the volume to 100 ml, then place it in an ultrasonic cleaner for 10 minutes to dissolve calcium chloride completely, and prepare 0.05 mol / L chlorine calcium solution.

[0081]S13, Preparation of 0.5...

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Abstract

The invention discloses an ultrahigh-flux platform for screening crystal generation conditions and a screening method. The platform comprises a micro-fluidic droplet chip, a micro-droplet collection chip and a characterization-analysis system, wherein the micro-fluidic droplet chip is used for generating crystallized droplets at ultrahigh flux; the micro-droplet collection chip is used for collecting the crystallized droplets generated by the micro-fluidic droplet chip; and after the micro-droplet collection chip collects the crystallized droplets, the characterization-analysis system characterizes and identifies the morphology of crystals in the crystallized droplets, and analyzes and screens out crystallization conditions corresponding to the optimal crystal morphology according to the characterized and identified morphology of the crystals. The ultrahigh-flux platform provided by the invention provides a good solution for rapid screening of crystal generation conditions. The screening method for screening the crystal generation conditions provided by the invention is based on the ultrahigh-flux platform, can quickly screen out excellent and stable crystallization conditions, andis convenient and accurate.

Description

technical field [0001] The invention relates to the technical field of microfluidic screening, in particular to an ultra-high-throughput platform and screening method for screening crystal formation conditions. Background technique [0002] Crystals are the focus of research in many fields, such as drug crystals, inorganic crystals, organometallic framework material crystals, organic crystals, etc. The physical and chemical properties of crystals in different crystal forms are very different. However, the crystallization of crystals is often affected by many parallel combination conditions, such as various combination solvents in different proportions (the classic single crystal culture solvent combination is: dichloromethane / toluene / n-hexane), in addition to temperature, pH, inducing reagents and other influencing factors. [0003] According to the mathematical permutation and combination method, the number of experiments required for the screening of crystal formation con...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N21/84G01N23/20008G01N25/14
CPCB01L3/5027G01N21/84G01N23/20008G01N25/147G01N2021/8477
Inventor 周建华苏振宁
Owner SUN YAT SEN UNIV
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