Bacillus sonorensis and application thereof

A technology of bacillus and bacteria agent, applied in Sonora desert bacillus and its application field, can solve the problems of increasing crop yield, achieve the effect of increasing yield and improving plant disease resistance

Active Publication Date: 2020-05-15
XINJIANG GENLIDUO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report that the soluble phosphorus content in the soil can be increased by us

Method used

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  • Bacillus sonorensis and application thereof
  • Bacillus sonorensis and application thereof
  • Bacillus sonorensis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0045] Example 1 Phosphorus dissolving function

[0046] Bacillus Sonora Desert Bacillus sonorensis The XW57 strain was activated on LB medium.

[0047] The activated strain was inoculated into the phosphorus-dissolving medium shown in Appendix A of NY / T 1847-2010 (glucose 10.00g, (NH 4 ) 2 SO 4 0.50 g, MnSO 4 ·7H 2 O 0.3g, NaCl 0.3g, KCl 0.30g, FeSO 4 ·4H 2 O 0.036g, MnSO 4 ·4H 2 O 0.03g, distilled water 1000 mL, pH 7.0. The inorganic phosphorus source is calcium phosphate with an addition amount of 10g; the organic phosphorus source is calcium phytate with an addition amount of 2g. Solid medium: add 1.5% agar powder in proportion).

[0048] After testing, Sonora Desert Bacillus Bacillus sonorensis The XW57 strain has the ability to dissolve both inorganic and organic phosphorus. The diameter of the dissolution circle that dissolves inorganic phosphorus reaches 1.3 cm. figure 1 As shown, the diameter of the dissolution circle for dissolving organic phosphorus reaches 1.4cm, se...

Example Embodiment

[0051] Example 2 Protease production

[0052] Bacillus Sonora Desert Bacillus sonorensis The XW57 strain was activated on LB medium.

[0053] Inoculate the activated strain into the protease detection medium (tryptone 5.00 g, yeast extract 3.00 g, glucose 1.00 g, agar 15.00 g, distilled water 1000 mL, pH 7.0, autoclave at 121°C for 30 min. Sterilize When the test culture medium is cooled to about 50 ℃, add skim milk to the culture medium at a ratio of 10%, mix it, pour it into a petri dish, wait for it to cool down, and incubate for 48 hours at 28 ℃ to observe whether there is a dissolution circle.

[0054] The results show that Bacillus Sonora Desert Bacillus sonorensis The XW57 strain has a good ability to solubilize protein, high protease production, and its dissolution circle diameter reaches 5.3cm, such as Figure 4 Shown. This strain has a high-yield protease and can be used for the extraction of protease, applied to the field of protease in the food industry or washing in...

Example Embodiment

[0055] Example 3 Cellulose production

[0056] Bacillus Sonora Desert Bacillus sonorensis The XW57 strain was activated on LB medium.

[0057] The activated strain was inoculated into the cellulose detection medium (MgSO 4 • 7H 2 O 0.25g, K 2 HPO 4 0.50g, 1.88g carboxymethylcellulose, 15.0g agar, 1000ml distilled water, pH 7.0, autoclaved at 121°C for 30 min). Incubate at 28°C for 7 days. After 7 days, add 5 mL of 0.2 mg / mL Congo red stain to each plate and stain for 1 hour. After discarding the Congo red stain, add 1 M NaCl and wash for 1 hour. Discard the washing solution and observe The formation of hydrolysis circles around the colony, the appearance of hydrolysis circles indicates the production of cellulase.

[0058] The results show that Bacillus Sonora Desert Bacillus sonorensis The XW57 strain has a good ability to dissolve cellulose, and its dissolution circle diameter reaches 6.0, such as Figure 5 Shown. The strain produces high cellulose, can degrade the cell wall...

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Abstract

The invention relates to bacillus sonorensis XW57. The bacillus sonorensis XW57 is deposited at the China Center for Type Culture Collection and has an accession number of CCTCC No: M2019819. The strain has a strong function of dissolving inorganic phosphorus and organic phosphorus, and can produce indoleacetic acid, cellulase and protease. The invention also relates to a microbial inoculum containing the strain and application of the strain in promoting plant growth and improving stress resistance such as plant disease resistance, drought resistance and the like.

Description

technical field [0001] The invention relates to a Sonora desert bacillus and its application, in particular to the function of the strain and its application in plant growth promotion. Background technique [0002] Rhizosphere growth-promoting bacteria are defined as bacteria living freely in the soil, rhizosphere, root surface, and phyllosphere that are beneficial to plant growth under certain conditions. These bacteria are able to fix nitrogen, dissolve phosphorus and iron, and produce plant hormones such as auxins, gibberellins, cytokinins, and ethylene. In addition, they increase plant resistance to stress, including drought, high salinity, heavy metal poisoning, and pesticides. [0003] Phosphorus is the second largest nutrient element necessary for plant growth and is involved in multiple metabolisms of plants, including energy transfer, signal transfer, respiration, synthesis of macromolecules and photosynthesis. However, 95%~99% of phosphorus is fixed by calcium io...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C05F11/08C12R1/07
CPCC12N1/20C05G3/00C05F11/08C12R2001/07C12N1/205
Inventor 吕硕邢明振柴同海
Owner XINJIANG GENLIDUO BIOTECH CO LTD
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