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Bacillus sonorensis and application thereof

A technology of bacillus and bacteria agent, applied in Sonora desert bacillus and its application field, can solve the problems of increasing crop yield, achieve the effect of increasing yield and improving plant disease resistance

Active Publication Date: 2020-05-15
XINJIANG GENLIDUO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report that the soluble phosphorus content in the soil can be increased by using phosphorus-solubilizing microorganisms, and then the yield of crops can be increased.

Method used

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  • Bacillus sonorensis and application thereof
  • Bacillus sonorensis and application thereof
  • Bacillus sonorensis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Phosphorus dissolving function

[0046] Bacillus desertis Bacillus sonorensis XW57 strain was activated on LB medium.

[0047] The activated bacterial strain is inoculated into phosphate-dissolving medium (glucose 10.00g, (NH 4 ) 2 SO 4 0.50 g, MnSO 4 ·7H 2 O 0.3g, NaCl 0.3g, KCl 0.30g, FeSO 4 4H 2 O 0.036g, MnSO 4 4H 2 O 0.03g, distilled water 1000mL, pH 7.0. The inorganic phosphorus source is calcium phosphate, and the addition amount is 10g; the organic phosphorus source is calcium phytate, and the addition amount is 2g. Solid medium: add 1.5% agar powder in proportion).

[0048] Sonora desert bacillus was detected Bacillus sonorensis The XW57 strain has the ability to dissolve both inorganic phosphorus and organic phosphorus, and the diameter of the dissolution circle for dissolving inorganic phosphorus reaches 1.3cm, see figure 1 As shown, the diameter of the dissolution circle for dissolving organic phosphorus reaches 1.4cm, see figure...

Embodiment 2

[0051] Example 2 Protease production

[0052] Bacillus desertis Bacillus sonorensis XW57 strain was activated on LB medium.

[0053] Inoculate the activated strain into protease detection medium (tryptone 5.00 g, yeast extract 3.00 g, glucose 1.00 g, agar 15.00 g, distilled water 1000 mL, pH 7.0, autoclave at 121°C for 30 min. Sterilize When the detection medium is cooled to about 50°C, add 10% skimmed milk into the medium and mix well, then pour it into a petri dish, wait for it to cool down for later use), incubate at 28°C for 48 hours, and observe whether there is a dissolution circle.

[0054] The results showed that Bacillus desertis Bacillus sonorensis The XW57 strain has a good ability to dissolve proteins, high-yield protease, and the diameter of its dissolution circle reaches 5.3cm, such as Figure 4 shown. This strain produces high-quality protease, which can be used for the extraction of protease, and can be used in the field of protease such as food industr...

Embodiment 3

[0055] Embodiment 3 produces cellulose

[0056] Bacillus desertis Bacillus sonorensis The XW57 strain was activated on LB medium.

[0057] The activated strain was inoculated into the medium for cellulose detection (MgSO 4 •7H 2 O 0.25g, K 2 HPO 4 0.50g, carboxymethylcellulose 1.88g, agar 15.0g, distilled water 1000mL, pH 7.0, autoclave at 121℃ for 30min). Incubate at 28°C for 7 days. After 7 days, add 5 mL of 0.2 mg / mL Congo red staining solution to each plate, stain for 1 hour, discard the Congo red staining solution, add 1 M NaCl to wash for 1 hour, discard the washing solution, and observe Production of a hydrolytic zone around the colony, the appearance of a hydrolytic zone indicates the production of cellulase.

[0058] The results showed that Bacillus desertis Bacillus sonorensis The XW57 strain has a good ability to dissolve cellulose, and the diameter of its dissolution circle reaches 6.0, such as Figure 5 shown. The strain produces high cellulose, can ...

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Abstract

The invention relates to bacillus sonorensis XW57. The bacillus sonorensis XW57 is deposited at the China Center for Type Culture Collection and has an accession number of CCTCC No: M2019819. The strain has a strong function of dissolving inorganic phosphorus and organic phosphorus, and can produce indoleacetic acid, cellulase and protease. The invention also relates to a microbial inoculum containing the strain and application of the strain in promoting plant growth and improving stress resistance such as plant disease resistance, drought resistance and the like.

Description

technical field [0001] The invention relates to a Sonora desert bacillus and its application, in particular to the function of the strain and its application in plant growth promotion. Background technique [0002] Rhizosphere growth-promoting bacteria are defined as bacteria living freely in the soil, rhizosphere, root surface, and phyllosphere that are beneficial to plant growth under certain conditions. These bacteria are able to fix nitrogen, dissolve phosphorus and iron, and produce plant hormones such as auxins, gibberellins, cytokinins, and ethylene. In addition, they increase plant resistance to stress, including drought, high salinity, heavy metal poisoning, and pesticides. [0003] Phosphorus is the second largest nutrient element necessary for plant growth and is involved in multiple metabolisms of plants, including energy transfer, signal transfer, respiration, synthesis of macromolecules and photosynthesis. However, 95%~99% of phosphorus is fixed by calcium io...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C05F11/08C12R1/07
CPCC12N1/20C05G3/00C05F11/08C12R2001/07C12N1/205
Inventor 吕硕邢明振柴同海
Owner XINJIANG GENLIDUO BIOTECH CO LTD
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