A non-glutaraldehyde prepackaged dry biological valve material and its preparation method and application

A bio-valve, glutaraldehyde technology, applied in tissue regeneration, medical science, prosthesis and other directions, can solve the problems of inability to protect elastin, glutaraldehyde aldehyde residues, and inability to spread quickly, and achieve good anti-calcification performance and Elastin stability, solving the effect of easy calcification, avoiding the problem of glutaraldehyde aldehyde residue

Active Publication Date: 2020-10-30
VENUS MEDTECH (HANGZHOU) INC
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a non-glutaraldehyde prepackaged dry biological valve material in order to solve the problems of residual glutaraldehyde aldehyde group, easy calcification, inability to protect elastin, and dry valve material that cannot be flattened quickly after being pressed. And preparation method and application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A non-glutaraldehyde prepackaged dry biological valve material and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A preferred embodiment of the present invention provides a method for preparing a non-glutaraldehyde preloaded dry biological valve material, the specific steps are as follows:

[0035] a. Soak the porcine pericardium in a 0.1wt% disodium ethylenediaminetetraacetic acid solution for 1h, then soak in a 0.1wt% sodium lauryl sulfate solution for 24h, then rinse in a sterile PBS solution for 1h .

[0036] b. Soak the decellularized pericardium material in a 5wt% glycidyl methacrylate solution and incubate at 37°C for 3 days to introduce a carbon-carbon double bond structure.

[0037] c. Soak the material obtained in the previous step in a 0.1M 3-sulfonic acid propyl methacrylate aqueous solution at 37°C and incubate for 24 hours.

[0038] d. Add 20mM ammonium persulfate and 20mM sodium bisulfite aqueous solution to the material obtained in the previous step for crosslinking at 37°C for 24h.

[0039] e. Use a mixed solution of 60mM carbodiimide and 12mM N-hydroxysuccinimide to immers...

Embodiment 2

[0043] A preferred embodiment of the present invention provides a method for preparing a non-glutaraldehyde preloaded dry biological valve material, the specific steps are as follows:

[0044] a. Soak the porcine pericardium in a 0.1wt% disodium ethylenediaminetetraacetic acid solution for 1h, then soak in a 0.1wt% sodium lauryl sulfate solution for 24h, then rinse in a sterile PBS solution for 1h .

[0045] b. Soak the acellular pericardium material in a 2wt% glycidyl methacrylate solution and incubate at 37°C for 3 days to introduce a carbon-carbon double bond structure.

[0046] c. Soak the material obtained in the previous step in a 0.1M 3-sulfonic acid propyl methacrylate aqueous solution at 37°C and incubate for 24 hours.

[0047] d. Add 20mM ammonium persulfate and 20mM sodium bisulfite aqueous solution to the material obtained in the previous step for crosslinking at 37°C for 24h.

[0048] e. Use a mixed solution of 60mM carbodiimide and 12mM N-hydroxysuccinimide to immerse the...

Embodiment 3

[0052] A preferred embodiment of the present invention provides a method for preparing a non-glutaraldehyde preloadable dry biological valve material, and the specific steps are as follows:

[0053] a. Soak the porcine pericardium in 0.2wt% disodium ethylenediaminetetraacetic acid solution for 1h, then soak in 0.2wt% sodium lauryl sulfate solution for 20h, and then rinse in sterile PBS solution for 3h .

[0054] b. Soak the decellularized pericardium material in an 8wt% glycidyl methacrylate solution and incubate at 37°C for 4 days to introduce a carbon-carbon double bond structure.

[0055] c. Soak the material obtained in the previous step in a 0.2M 3-sulfonic acid propyl methacrylate aqueous solution at 37°C and incubate for 24 hours.

[0056] d. Add 20mM ammonium persulfate and 20mM sodium bisulfite aqueous solution to the material obtained in the previous step for crosslinking at 37°C for 24h.

[0057] e. Use a mixed solution of 50 mM carbodiimide and 15 mM N-hydroxysuccinimide to...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention discloses a non-glutaraldehyde pre-installable dry biological valve material and a preparation method and an application thereof, and belongs to the technical field of medical materials. The preparation method comprises the following specific steps: a, decellularizing a pig or bovine pericardium material; b, soaking the decellularized pericardium in a glycidyl methacrylate oracrylic anhydride solution to introduce a carbon-carbon double bond structure; c, soaking the material in an aqueous solution of propyl methacrylate 3-sulfonate; d, adding an initiator for double bond polymerization cross-linking; e, using a mixed solution of carbodiimide and N-hydroxysuccinimide for cross-linking fixation; f, soaking the material in a polyphenol solution; and g, conducting storage with a micro-organism-inhibiting solution after rinsing or with an alcohol solution after dehydration drying. The preparation method can avoid problems of glutaraldehyde aldehyde group residues, easy calcification, and inability to protect elastin, at the same time, can effectively solve a problem that the dry valve material cannot be quickly flattened after being pressed and held, and potentially extends service life.

Description

Technical field [0001] The invention belongs to the technical field of medical materials, and specifically relates to a non-glutaraldehyde preloadable dry biological valve material, and a preparation method and application thereof. Background technique [0002] The current artificial biological heart valve materials on the market mainly use porcine or bovine pericardium as raw materials and are made by cross-linking with glutaraldehyde solution. In addition, the artificial biological valve prepared from the pericardium cross-linked by glutaraldehyde has a current service life of only about 10 years due to the residual glutaraldehyde aldehyde group, calcification, and the inability to protect elastin. Based on this, the development of new biological valve materials has important clinical value and social significance. [0003] In order to overcome the shortcomings of glutaraldehyde cross-linked biological heart valves, many methods have been used to improve or replace glutaraldehyd...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3625A61L27/3687A61L27/3691A61L27/50A61L2430/20A61L2430/40
Inventor 雷洋王云兵郭高阳杨立
Owner VENUS MEDTECH (HANGZHOU) INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap