A non-glutaraldehyde prepackaged dry biological valve material and its preparation method and application
A bio-valve, glutaraldehyde technology, applied in tissue regeneration, medical science, prosthesis and other directions, can solve the problems of inability to protect elastin, glutaraldehyde aldehyde residues, and inability to spread quickly, and achieve good anti-calcification performance and Elastin stability, solving the effect of easy calcification, avoiding the problem of glutaraldehyde aldehyde residue
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Embodiment 1
[0034] A preferred embodiment of the present invention provides a method for preparing a non-glutaraldehyde preloaded dry biological valve material, the specific steps are as follows:
[0035] a. Soak the porcine pericardium in a 0.1wt% disodium ethylenediaminetetraacetic acid solution for 1h, then soak in a 0.1wt% sodium lauryl sulfate solution for 24h, then rinse in a sterile PBS solution for 1h .
[0036] b. Soak the decellularized pericardium material in a 5wt% glycidyl methacrylate solution and incubate at 37°C for 3 days to introduce a carbon-carbon double bond structure.
[0037] c. Soak the material obtained in the previous step in a 0.1M 3-sulfonic acid propyl methacrylate aqueous solution at 37°C and incubate for 24 hours.
[0038] d. Add 20mM ammonium persulfate and 20mM sodium bisulfite aqueous solution to the material obtained in the previous step for crosslinking at 37°C for 24h.
[0039] e. Use a mixed solution of 60mM carbodiimide and 12mM N-hydroxysuccinimide to immers...
Embodiment 2
[0043] A preferred embodiment of the present invention provides a method for preparing a non-glutaraldehyde preloaded dry biological valve material, the specific steps are as follows:
[0044] a. Soak the porcine pericardium in a 0.1wt% disodium ethylenediaminetetraacetic acid solution for 1h, then soak in a 0.1wt% sodium lauryl sulfate solution for 24h, then rinse in a sterile PBS solution for 1h .
[0045] b. Soak the acellular pericardium material in a 2wt% glycidyl methacrylate solution and incubate at 37°C for 3 days to introduce a carbon-carbon double bond structure.
[0046] c. Soak the material obtained in the previous step in a 0.1M 3-sulfonic acid propyl methacrylate aqueous solution at 37°C and incubate for 24 hours.
[0047] d. Add 20mM ammonium persulfate and 20mM sodium bisulfite aqueous solution to the material obtained in the previous step for crosslinking at 37°C for 24h.
[0048] e. Use a mixed solution of 60mM carbodiimide and 12mM N-hydroxysuccinimide to immerse the...
Embodiment 3
[0052] A preferred embodiment of the present invention provides a method for preparing a non-glutaraldehyde preloadable dry biological valve material, and the specific steps are as follows:
[0053] a. Soak the porcine pericardium in 0.2wt% disodium ethylenediaminetetraacetic acid solution for 1h, then soak in 0.2wt% sodium lauryl sulfate solution for 20h, and then rinse in sterile PBS solution for 3h .
[0054] b. Soak the decellularized pericardium material in an 8wt% glycidyl methacrylate solution and incubate at 37°C for 4 days to introduce a carbon-carbon double bond structure.
[0055] c. Soak the material obtained in the previous step in a 0.2M 3-sulfonic acid propyl methacrylate aqueous solution at 37°C and incubate for 24 hours.
[0056] d. Add 20mM ammonium persulfate and 20mM sodium bisulfite aqueous solution to the material obtained in the previous step for crosslinking at 37°C for 24h.
[0057] e. Use a mixed solution of 50 mM carbodiimide and 15 mM N-hydroxysuccinimide to...
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