Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for determining non-volatile organic acids and fatty acids in tobacco by using miniature extraction separation-gas chromatography

A gas chromatography, non-volatile technology, applied in the field of micro-extraction separation-gas chromatography for the determination of non-volatile organic acids and fatty acids in tobacco, can solve the problems of time-consuming operation, large consumption of organic solvents, etc., and achieves easy operation and organic solvent. The effect of reduced consumption and easy promotion and application

Active Publication Date: 2020-05-19
CHINA TOBACCO YUNNAN IND
View PDF16 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of methyl esterification with sulfuric acid-methanol is the most commonly used method. This method is to dissolve the organic acid in the sample by using sulfuric acid-methanol solution. The operation needs to add the tobacco sample into the sulfuric acid-methanol solution and reflux for several hours. The operation is very time-consuming and consumes a lot of organic solvents.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for determining non-volatile organic acids and fatty acids in tobacco by using miniature extraction separation-gas chromatography
  • Method for determining non-volatile organic acids and fatty acids in tobacco by using miniature extraction separation-gas chromatography
  • Method for determining non-volatile organic acids and fatty acids in tobacco by using miniature extraction separation-gas chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The method for micro-extraction separation-gas chromatography determination of non-volatile organic acids and fatty acids in tobacco comprises the steps:

[0044] Step (1), sample pretreatment:

[0045] The tobacco sample to be tested is pulverized and placed in a microwave reaction tank, and then an internal standard solution and a sulfuric acid methanol solution are added thereto, and after microwave reaction, filtered to obtain a filtrate;

[0046] Described internal standard is adipic acid;

[0047] Step (2), micro-extraction separation:

[0048] Take the filtrate obtained in step (1) and mix it with saturated sodium chloride solution, then transfer it to a micro-extraction device, use dichloromethane as the extraction agent to carry out vibration extraction, and after standing for stratification, remove the lower layer of clear liquid;

[0049] Step (3), gas chromatography analysis and quantification:

[0050] The lower layer liquid obtained in step (2) is analy...

Embodiment 2

[0053] The method for micro-extraction separation-gas chromatography determination of non-volatile organic acids and fatty acids in tobacco comprises the steps:

[0054] Step (1), sample pretreatment:

[0055] The tobacco sample to be tested is pulverized and placed in a microwave reaction tank, and then an internal standard solution and a sulfuric acid methanol solution are added thereto, and after microwave reaction, filtered to obtain a filtrate;

[0056] Described internal standard is adipic acid;

[0057] Step (2), micro-extraction separation:

[0058] Take the filtrate obtained in step (1) and mix it with saturated sodium chloride solution, then transfer it to a micro-extraction device, use dichloromethane as the extraction agent to carry out vibration extraction, and after standing for stratification, remove the lower layer of clear liquid;

[0059] Step (3), gas chromatography analysis and quantification:

[0060] The lower layer liquid obtained in step (2) is analy...

Embodiment 3

[0073] The method for micro-extraction separation-gas chromatography determination of non-volatile organic acids and fatty acids in tobacco comprises the steps:

[0074] Step (1), sample pretreatment:

[0075] The tobacco sample to be tested is pulverized and placed in a microwave reaction tank, and then an internal standard solution and a sulfuric acid methanol solution are added thereto, and after microwave reaction, filtered to obtain a filtrate;

[0076] Described internal standard is adipic acid;

[0077] Step (2), micro-extraction separation:

[0078] Take the filtrate obtained in step (1) and mix it with saturated sodium chloride solution, then transfer it to a micro-extraction device, use dichloromethane as the extraction agent to carry out vibration extraction, and after standing for stratification, remove the lower layer of clear liquid;

[0079] Step (3), gas chromatography analysis and quantification:

[0080] The lower layer liquid obtained in step (2) is analy...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for determining non-volatile organic acids and fatty acids in tobacco by using miniature extraction separation-gas chromatography, belonging to the technical field ofanalytical chemistry. The method comprises the following steps: crushing a tobacco sample to be detected, putting the crushed tobacco sample into a microwave reaction tank, adding an internal standard solution and a methanol solution of sulfuric acid into the microwave reaction tank, carrying out a microwave reaction, and conducting filtering to obtain a filtrate, wherein internal standard is adipic acid; mixing the filtrate with a saturated sodium chloride solution, transferring the obtained mixture into a miniature extraction device, carrying out oscillation extraction by taking dichloromethane as an extraction agent, conducting standing for layering, and taking subnatant; and carrying out gas chromatographic analysis on the obtained subnatant, and conducting quantifying by using an external standard method. Compared with a conventional method, the method disclosed by the invention has the advantages that extraction and derivation reaction speeds are greatly increased and can be completed in only 10 minutes; the consumption of derivation reagents and extraction organic solvents is greatly reduced; and the method is simple and convenient to operate, high in accuracy, good in repeatability, suitable for analysis of large-batch samples and easy to popularize and apply.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, and in particular relates to a micro-extraction separation-gas chromatography method for measuring non-volatile organic acids and fatty acids in tobacco. Background technique [0002] There are a variety of organic acids in tobacco. It is generally believed that organic acids in tobacco leaves can be divided into volatile and non-volatile (except amino acids) and some higher fatty acids. Most of the relatively high content are dibasic acids in non-volatile organic acids. and tribasic acids, the content of malic acid, citric acid, oxalic acid and malonic acid is relatively high (accounting for about 5% to 18% in flue-cured tobacco), and the non-volatile organic acids and fatty acids in tobacco have an important impact on the quality of tobacco . Therefore, the determination of non-volatile organic acids, higher fatty acids and their derivatives in tobacco has gradually become the con...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): B01D11/02B01D11/04B01J19/12G01N30/02G01N30/06G01N30/14
CPCB01D11/0211B01D11/0419G01N30/02G01N30/06G01N30/14G01N2030/062G01N2030/067
Inventor 李超李娥贤张承明姜黎王庆华吴佳高文军
Owner CHINA TOBACCO YUNNAN IND