Method for preparing glyceride rich in arachidonic acid by two-step enzyme method, and product prepared by method

A technology of glyceryl tetraenoic acid and arachidonic acid, applied in the field of oil processing, can solve the problems of low content of arachidonic acid, no reports on preparation, etc., and achieve the effects of improving digestion and absorption efficiency and simple process

Pending Publication Date: 2020-05-19
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to existing reports, the raw materials for the synthesis of partial glycerides mainly come from common vegetable oils, such as soybean oil, rapeseed oil, etc., but the preparation of partial glycerides containing arachidonic acid has not been reported, and because the content of arachidonic acid in microbial oils is less than 40%. or so, the content of arachidonic acid in some glycerides obtained by the existing technology is relatively low

Method used

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  • Method for preparing glyceride rich in arachidonic acid by two-step enzyme method, and product prepared by method
  • Method for preparing glyceride rich in arachidonic acid by two-step enzyme method, and product prepared by method
  • Method for preparing glyceride rich in arachidonic acid by two-step enzyme method, and product prepared by method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) The microbial oil rich in arachidonic acid and ethanol are mixed according to the molar ratio of 1:3, add in the sealable batch reactor, and add the Lipozyme RM IM of 12wt% wherein, at temperature The fatty acid composition of the obtained intermediate enzymatic hydrolysis product is shown in Table 1 below under the condition of 25° C. and a stirring rate of 400 rpm for 4 hours.

[0028] The fatty acid composition of table 1 intermediate enzymatic hydrolysis product

[0029]

[0030]

[0031] (2) After vacuum distillation and molecular distillation are used to remove the ethanol and fatty acid ethyl ester of the intermediate enzymatic hydrolysis product respectively, the intermediate enzymatic hydrolysis product and glycerin are mixed in a batch reactor according to the molar ratio of 1:3, and are added to the Add 3wt% Novozym 435, and react for 18 hours at a temperature of 70° C. and a stirring rate of 600 rpm. The composition of the obtained monoglyceride pr...

Embodiment 2

[0045] (1) The microbial oil rich in arachidonic acid and ethanol are mixed according to the molar ratio of 1:6, add in the sealable batch reactor, and add the Lipozyme TL IM of 8wt% wherein, at temperature The reaction was sealed for 8 hours at 35°C and a stirring rate of 600 rpm, and the fatty acid composition of the obtained intermediate enzymatic hydrolysis product was shown in Table 6 below.

[0046] The fatty acid composition of table 6 intermediate enzymatic hydrolysis product

[0047]

[0048]

[0049] (2) After vacuum distillation and molecular distillation are used to remove the ethanol and fatty acid ethyl ester of the intermediate enzymatic hydrolysis product respectively, the intermediate enzymatic hydrolysis product and glycerol are mixed in a batch reactor according to the ratio of 1:5 in molar ratio, and poured into it Add 8wt% Novozym 435 and react for 12 hours at a temperature of 50° C. and a stirring rate of 600 rpm. The composition of the obtained mon...

Embodiment 3

[0063] (1) The microbial oil rich in arachidonic acid and ethanol are mixed according to the molar ratio of 1:9, add in the sealable batch reactor, and add 4wt% Lipase AY-30SD wherein, at temperature The fatty acid composition of the obtained intermediate enzymolysis product is shown in Table 11 below under the condition of 45°C and a stirring rate of 800rpm for 12 hours.

[0064] Fatty acid composition of table 11 intermediate enzymatic hydrolysis product

[0065]

[0066] (2) After vacuum distillation and molecular distillation are used to remove the ethanol and fatty acid ethyl ester of the intermediate enzymatic hydrolysis product respectively, the intermediate enzymatic hydrolysis product and glycerol are mixed in a batch reactor according to the ratio of 1:6 in molar ratio, and poured into it Add 12wt% of Novozym 435 and react for 6 hours at a temperature of 30°C and a stirring rate of 400rm. The composition of the obtained monoglyceride product is shown in Table 12 b...

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Abstract

The invention discloses a method for preparing glyceride rich in arachidonic acid by a two-step enzyme method, and a product prepared by the method. The method comprises the steps of performing an enzyme catalysis and alcoholysis reaction to obtain an intermediate enzymolysis product rich in arachidonic acid; adding the intermediate enzymolysis product and glycerine according to the molar ratio being 1 to (3-6) into a batch reactor, adding 3-12wt% of lipase, and performing a reaction under the condition of temperature being 30-70 DEG C and stirring rate being 400-800rpm for 6-18 hours to obtain a monoglyceride product; and adding the intermediate enzymolysis product and the glycerine according to the molar ratio being 1 to (0.5-1.5) into the batch reactor, adding 6-15wt% of lipase, and performing a reaction under the condition of temperature being 30-70 DEG C and stirring rate being 400-800rpm for 4-12 hours so as to obtain a diglyceride product. According to the method disclosed by the invention, the condition is optimized, and the monoglyceride product rich in arachidonic acid or the diglyceride product rich in arachidonic acid is prepared; and besides, the method is simple in technology, convenient to implement and easy to popularize.

Description

technical field [0001] The invention belongs to the technical field of oil processing, and specifically relates to a two-step enzymatic method for preparing glyceride rich in arachidonic acid and a product thereof. Background technique [0002] Arachidonic acid (C20:4n-6) is highly concentrated in the phospholipid bilayers of the brain and retinal nerve membranes of animals, and is of great significance to phototransduction and neuron function. Adults are able to synthesize arachidonic acid endogenously from linoleic acid (C18:2n-6), however, the ability to endogenously synthesize arachidonic acid is limited and varies widely between individuals, and for some infants, The ability to synthesize arachidonic acid is close to zero. Therefore, arachidonic acid is an essential fatty acid for infants and is mainly dependent on dietary supply. Human milk, considered an ideal food for infants, contains small amounts of arachidonic acid. The global average of arachidonic acid in hu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
CPCC12P7/6472C12P7/6454
Inventor 邹孝强张晖金青哲
Owner JIANGNAN UNIV
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