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Heat-resistant protective agent for Newcastle disease-bronchitis combined live vaccine and preparation process of heat-resistant protective agent

A technology of heat-resistant protective agent and bronchitis, which is applied in the direction of vaccines, multivalent vaccines, antiviral agents, etc., and can solve problems such as unstable performance and low yield rate

Pending Publication Date: 2020-05-22
LIAONING YIKANG BIOLOGICAL CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Research on heat-resistant freeze-dried protective agents for chicken Newcastle disease and chicken infectious bronchitis dual live vaccines. The freeze-dried vaccines that use 5% sucrose skimmed milk as protective agents are often used. The performance of the product is not stable enough, and the pass rate is low.

Method used

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  • Heat-resistant protective agent for Newcastle disease-bronchitis combined live vaccine and preparation process of heat-resistant protective agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1) First, heat 500ml of water for injection to boiling, slowly add 30g of calcium lactate, stir until it is completely dissolved and transparent, and filter with qualitative filter paper; then add 10g of thiourea, 20g of sucrose, 4g of trehalose, 6g of sodium L-glutamate and beef Dissolve 16g of serum albumin in calcium lactate solution, make up to 800ml with water for injection, and then filter and sterilize to complete the preparation of liquid A;

[0023] 2) Heat 100mL of water for injection to boiling, then add 5g of polyvinylpyrrolidone, add 4g of d-mannitol, stir until dissolved and transparent; pass the condition of autoclaving at 15 pounds for 15 minutes, and make up to 200mL with water for injection , complete the preparation of liquid B;

[0024] 3) Mix liquid A, liquid B and liquid C together according to the volume ratio of 4:1 to make a heat-resistant protective agent;

[0025] 4) The vaccine freeze-dried protective agent is mixed with the virus solution o...

Embodiment 2

[0028] 1) First, heat 500ml of water for injection to boiling, slowly add 25g of calcium lactate, stir until it is completely dissolved and transparent, and filter with qualitative filter paper; then add 7g of thiourea, 16g of sucrose, 5g of trehalose, 10g of sodium L-glutamate and beef Dissolve 20g of serum albumin in calcium lactate solution, make up to 800ml with water for injection, and then filter and sterilize to complete the preparation of liquid A;

[0029] 2) Heat 100mL of water for injection to boiling, then add 6g of polyvinylpyrrolidone, add 5g of d-mannitol, stir until dissolved and transparent; pass the condition of autoclaving at 15 pounds for 15 minutes, and make up to 200mL with water for injection , complete the preparation of liquid B;

[0030] 3) Mix liquid A, liquid B and liquid C together according to the volume ratio of 4:1 to make a heat-resistant protective agent;

[0031] 4) The vaccine freeze-dried protective agent is mixed with the virus solution o...

Embodiment 3

[0034] 1) First, heat 500ml of water for injection to boiling, slowly add 20g of calcium lactate, stir until completely dissolved and transparent, and filter with qualitative filter paper; then sequentially add 5g of thiourea, 15g of sucrose, 5g of trehalose, 6g of sodium L-glutamate and milk Dissolve 13g of serum albumin in calcium lactate solution, make up to 800ml with water for injection, and then filter and sterilize to complete the preparation of liquid A;

[0035] 2) Heat 100mL of water for injection to boiling, then add 4g of polyvinylpyrrolidone, add 3g of d-mannitol, stir until dissolved and transparent; pass the condition of autoclaving at 15 pounds for 15 minutes, and make up to 200mL with water for injection , complete the preparation of liquid B;

[0036] 3) Mix liquid A, liquid B and liquid C together according to the volume ratio of 4:1 to make a heat-resistant protective agent;

[0037] 4) The vaccine freeze-dried protective agent is mixed with the virus solu...

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Abstract

The invention relates to the technical field of vaccine production for animals, and in particular to a heat-resistant protective agent for a Newcastle disease-bronchitis combined live vaccine and a preparation process of the heat-resistant protective agent. The protective agent consists of liquid A and liquid B according to a volume ratio of 4:1; the liquid A is composed of 20-30 g of calcium lactate, 5-10 g of thiourea, 15-20 g of sucrose, 3-5 g of trehalose, 5-10 g of sodium L-glutamate and 10-20 g of bovine serum albumin; the liquid A is supplemented to 800 ml with water for injection; theliquid B consists of: 4-6 g of polyvinylpyrrolidone and 3-5 g of d-mannitol, and the liquid B is supplemented to 200 ml with water for injection. During use, the protective agent is mixed with Newcastle disease virus and infectious bronchitis virus antigens according to a ratio of 1:1, and the mixture is subpackaged, and freeze-dried. Compared with virus content of an efficacy test, drop in content of Newcastle disease virus and infectious bronchitis virus in one dosage per chicken should not exceed 1 titer after being placed at 37 DEG C for 10 days, and all test results are qualified. The protective agent prepared by the preparation process provided by the invention has stable performance and high yield.

Description

technical field [0001] The invention relates to the technical field of animal vaccine production, in particular to a heat-resistant protective agent for chicken Newcastle disease and bronchitis dual live vaccine and a preparation process thereof. Background technique [0002] Lyoprotectant and its preparation process are a key technology in the production of live vaccines, which have a great impact on the storage, transportation and use of vaccines. At present, in my country's veterinary vaccines, live freeze-dried vaccines account for a considerable proportion. The commonly used protective agents in China are mainly milk, sucrose, gelatin, etc., which have simple formulas and poor protective functions for vaccines. Freeze-dried vaccines using these traditional protective agents usually require products to be stored below -15°C, which causes great inconvenience to manufacturers and users, restricts long-term storage and transportation of vaccines, and may be due to improper ...

Claims

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Application Information

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IPC IPC(8): A61K47/42A61K47/12A61K47/18A61K47/20A61K47/26A61K47/32A61K47/10A61K9/19A61K39/295A61K39/215A61K39/17A61P31/14
CPCA61K9/19A61K47/42A61K47/26A61K47/12A61K47/183A61K47/20A61K39/12A61K47/32A61K47/10A61P31/14A61K2039/70C12N2760/18134C12N2770/20034
Inventor 陈生雷吉莉莉李尚波
Owner LIAONING YIKANG BIOLOGICAL CORP LTD