Supercharge Your Innovation With Domain-Expert AI Agents!

Preparation method of ratiometric fluorescent aptamer sensor for detecting zearalenone

An aptamer sensor, zearalenone technology, applied in the direction of fluorescence/phosphorescence, instruments, measuring devices, etc., can solve the problems that have not been reported, and achieve the effect of good selectivity, low cost and high sensitivity

Active Publication Date: 2020-06-02
JIANGSU UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on the use of ratiometric fluorescent aptasensors based on dual-signal output mode for ZEN analysis and detection has not been reported yet.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of ratiometric fluorescent aptamer sensor for detecting zearalenone
  • Preparation method of ratiometric fluorescent aptamer sensor for detecting zearalenone
  • Preparation method of ratiometric fluorescent aptamer sensor for detecting zearalenone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] according to figure 1 In the preparation process, the preparation method of the ratio fluorescent aptamer sensor includes the following steps:

[0038] (1) Synthesis of CdTe QDs;

[0039] Under vigorous stirring, add 0.1142g CdCl 2 ·2.5H 2 Dissolve O in 50mL water, add 75μL mercaptopropionic acid, then use 1mol L -1 Adjust the pH of the solution to 8.5 with NaOH, bubbling with nitrogen for 15 minutes; then, quickly add 2 mL with 0.0646g Te and 0.0457g NaBH 4 Synthesized precursor NaHTe solution; then pour the mixed solution into a three-necked flask after 10 minutes of nitrogen gas, reflux at 100°C for 20 hours to obtain red CdTe QDs with an emission wavelength of 665nm, and add an equal volume of ethanol to centrifuge to remove impurities; After reconstitution, it is dissolved in secondary water and stored at 4°C.

[0040] (2) Synthesis of CdTe@SiO 2

[0041] Mix 1mL 10μM CdTe QDs solution with 6mL ethanol, and stir at room temperature for 5min; then, add 20μLAPTES, and the mi...

Embodiment 2

[0052] Draw ZEN response standard curve and linear regression equation:

[0053] Steps (1) to (5) follow the steps (1) to (5) of Example 1. The difference is that after adding MTX, let it act for 20 minutes, and add 0, 0.001, 0.002, 0.005, 0.02, 0.1, 0.2, 1nM ZEN standard solution, react for 40 minutes. Detect the fluorescence intensity at 435nm and 687nm of the above-mentioned ZEN standard solution of different concentrations with a fluorescence spectrophotometer at room temperature, and the resulting spectrum is as follows image 3 As shown in A, the curve in the figure from top to bottom corresponds to the concentration of ZEN standard solution as 0, 0.001, 0.002, 0.005, 0.02, 0.1, 0.2, 1nM. When 0nM ZEN is added, the fluorescence signal ratio of 687nm and 435nm is recorded as (F 687 / F 435 ) 0 , When other concentrations of ZEN are added, the fluorescence signal ratio of 687nm to 435nm is recorded as (F 687 / F 435 ), get (F 687 / F 435 ) / (F 687 / F 435 ) 0 (With I d Representat...

Embodiment 3

[0055] Investigation of ZEN detection selectivity:

[0056] Prepare the proportional fluorescence aptamer sensor according to the steps in Example 1. Add 30 μL 3nM aflatoxin B1 (AFB1), 3nM fumonisin B1 (FB1), 3nM ochre into 270μL of the ratio fluorescence aptamer sensor for detecting ZEN. Aspergillus toxin (OTA), 0.2nM ZEN and 3nM AFB1, FB1, OTA mixture, after 40 minutes of action, use a fluorescence spectrophotometer at room temperature to detect the fluorescence intensity of the different solutions at 435nm and 687nm. Such as Figure 4 As shown, AFB1, FB1, and OTA have no significant effect on the fluorescence intensity of the dual-signal fluorescent probe, and when ZEN is introduced into the system, the fluorescence signal will be significantly quenched. The above results indicate that the ratio fluorescent aptamer sensing system can realize the specific detection of ZEN.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of aptamer sensing detection, and discloses a preparation method of a ratiometric fluorescence aptamer sensor for detecting zearalenone (ZEN). The invention belongs to an aptamer sensor based on an internal filtering effect. The fluorescence intensity of the CdTe (at) SiO2 and the aptamer modified NGQDs is used as a double-output signal; therefore, high-sensitivity, low-cost and high-selectivity detection of ZEN in an actual sample is realized by utilizing the interaction between mitoxantrone (MTX) and an aptamer, the internal rate effect between MTX and CdTe-(at) SiO2 and the characteristic that the aptamer can be specifically combined with a target object ZEN as a biological recognition element. The response range of the built ratiometric fluorescence aptamer sensor to ZEN is 0.001-1nM, the detection limit is 0.33 pM, and the ratiometric fluorescence aptamer sensor has the advantages of high sensitivity, good selectivity and low cost, andthe invention provides a novel aptamer sensing platform for determining ZEN in an actual sample.

Description

Technical field [0001] The invention belongs to the technical field of biosensing detection, and specifically relates to a method for preparing a ratio fluorescent aptamer sensor for detecting zearalenone, and using it for selective detection of zearalenone (ZEN). Background technique [0002] Zearalenone (ZEN) is a secondary metabolite produced by fungal infection. It is produced by Fusarium graminearum and Fusarium spores, and is widely present in various crops such as corn, wheat, barley and sorghum. Due to its reproductive and neurotoxicity, and even carcinogenicity, ZEN is classified as the third carcinogen by the International Agency for Research on Cancer (IARC). In order to avoid the occurrence of ZEN poisoning incidents, many countries and institutions have stipulated the maximum limit of ZEN in different crops and their products. Chinese national standards require that the maximum limit of ZEN for corn and wheat products is 60μg Kg. -1 . Therefore, it is necessary to d...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/64
CPCG01N21/64G01N21/6428G01N2021/6421G01N2021/6432
Inventor 由天艳毕晓雅李丽波刘晓红陈柏年
Owner JIANGSU UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com