Vacuum-assisted nano-liter liquid chromatographic column filling device and filling method
A liquid chromatography column and vacuum-assisted technology, which is applied in the field of vacuum-assisted nanoliter liquid chromatography column filling devices, can solve the problems of environmental pollution and low filling speed of capillary packing, and achieves small volume, eliminates pollution, and improves filling speed. Effect
Pending Publication Date: 2020-06-02
上海易质谱科技有限公司
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Problems solved by technology
[0004] Common capillary column packing instruments on the market generally place the tip of the capillary in the air, which makes the filling speed of the capillary packing lower; moreover, some of the existing capillary column packing instruments are shortened and placed in the air, and the Exhaust gas and waste liquid are directly discharged into the air, which will cause environmental pollution
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[0029] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further elaborated below in conjunction with illustrations and specific embodiments.
[0030] Such as figure 1 As shown, the vacuum-assisted nanoliter liquid chromatography column filling device proposed by the present invention includes a nitrogen bottle 1 and a capillary column packing instrument 2 connected with the nitrogen bottle 1, and the capillary column packing instrument 2 passes through the capillary chromatographic column. 3 is connected with the vacuum cavity 4, and the vacuum cavity 4 is also connected with the vacuum pump 5;
[0031] A 1.8ml centrifuge tube 6 is fixed inside the capillary column packing instrument 2, and the centrifuge tube 6 is filled with filler and methanol suspension, and the end of the capillary chromatographic column 3 is inserted into the bottom of the centrifuge tube 6.
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The invention relates to the technical field of chromatographic column filling, and adopts the technical scheme that the vacuum-assisted nano-liter liquid chromatographic column filling device comprises a nitrogen cylinder and a capillary column filling instrument connected with the nitrogen cylinder; the capillary column filling instrument is connected with a vacuum cavity through a capillary chromatographic column, and the vacuum cavity is further connected with a vacuum pump; a 1.8 ml centrifuge tube is fixed in the capillary column packing instrument, the centrifuge tube is filled with filler and methanol turbid liquid, and the tail end of the capillary chromatographic column is inserted into the bottom of the centrifuge tube. According to the invention, the vacuum cavity device is small in size and can quickly reach an ideal vacuum state; the pressure of the vacuum pump can be adjusted to conveniently control the seasoning filling speed; waste gas and waste liquid are pumped awaythrough the vacuum pump, filtered and then discharged into air, and no waste liquid is generated any more; meanwhile, the waste gas is filtered, and the pollution to the environment can be almost eliminated.
Description
technical field [0001] The invention relates to the technical field of chromatographic column filling, in particular to a vacuum-assisted nanoliter liquid chromatographic column filling device and a filling method. Background technique [0002] Liquid chromatography-mass spectrometry technology is widely used to detect the composition and content of substances in complex samples, and the quality and stability of the results depend to a large extent on the important component of the chromatographic column. In high-throughput proteomics analysis, an important application field of liquid chromatography-mass spectrometry, the most common liquid chromatography device is nanoliter liquid chromatography and matching capillary column. This system has good reproducible results due to its low flow rate. It is regarded as the "gold standard" in related research fields. The column efficiency of the capillary chromatographic column will gradually decrease as the number of detections inc...
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Login to View More IPC IPC(8): G01N30/56
CPCG01N30/56G01N2030/562
Inventor 郭方李振
Owner 上海易质谱科技有限公司