Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Biological inhibitor for silver carp surimi gel degradation

A technology for silver carp and fish meat is applied in the field of biological inhibitors for the deterioration of silver carp surimi gel, can solve the problems of high cost, less research, unfavorable digestion and absorption, etc., and achieves high thermal stability, high acid and alkali resistance, specificity strong effect

Active Publication Date: 2020-06-09
OCEAN UNIV OF CHINA
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since MBSP is widely present in fish muscle tissue, it cannot be removed by rinsing during the production of surimi, which seriously restricts the development of surimi products industry.
[0003] At present, research on MBSP inhibition is mainly divided into two categories: ①Inhibition of the serine protease family where MBSP belongs: such as ovalbumin, animal plasma protein, potato starch, bean trypsin inhibitors, etc. can significantly inhibit the activity of serine protease, but due to Its high cost, affecting the color and flavor of surimi products, is not conducive to human digestion and absorption and other defects limit its application in actual production
②Specific inhibitors for MBSP: There are few studies on such inhibitors, mainly because of the inhibitory effect of endogenous glucose-6-phosphate isomerase (GPI) in fish on MBSP. These GPIs only have the effect on MBSP of the same fish Inhibitory, not broad-spectrum specific
Due to the lack of MBSP inhibitors, most surimi production at this stage adopts the method of adding transglutaminase (TG) enzyme to improve the gel strength of surimi products, but the catalysis of TG enzyme requires high processing conditions, and it is necessary to add Non-muscle proteins work together to enhance the effect, affecting the properties of the surimi itself

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biological inhibitor for silver carp surimi gel degradation
  • Biological inhibitor for silver carp surimi gel degradation
  • Biological inhibitor for silver carp surimi gel degradation

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0032] A biological inhibitor for silver carp surimi gel deterioration, comprising the aforementioned shark-derived single domain antibody. The preparation method of the shark-derived single domain antibody comprises the following steps:

[0033] 1) Immunize the hinged shark with silver carp myofibril-binding serine protease as an antigen, and measure the immune response of the shark; collect peripheral blood lymphocytes of highly immune sharks, extract RNA and synthesize a cDNA library, and use gene recombination technology to construct phage The plasmid vector was introduced into Escherichia coli to construct the shark-derived single domain antibody gene text library and identify the capacity of the text library.

[0034] 2) Use helper phagemids to infect the single-domain antibody gene text library, use silver carp fish myofibril-binding serine protease as the antigen, use phage display technology to pass multiple rounds of panning, and obtain the single-domain antibody wit...

specific Embodiment

[0036] Specific example: Preparation of silver carp fish meat myofibril-binding serine protease (MBSP) specific shark-derived single domain antibody and inhibition of silver carp surimi gel degradation effect

[0037] 1. Construction of MBSP shark-derived single-domain antibody text library for silver carp meat

[0038] The silver carp meat MBSP prepared in the laboratory was fully mixed with Freund's adjuvant 1:1, and then subcutaneously injected into the dorsal fin to immunize the mouth shark. The immunization was boosted once every two weeks, and the shark serum was collected after four times of immunization. Response effect of MBSP serum; collected shark peripheral blood lymphocytes that responded efficiently after immunization, extracted total RNA by TRIzol method, identified RNA quality by Experion, synthesized cDNA library using SuperScript system after TURBO TMDnase treatment, designed single-domain antibody bidirectional primers, and passed PCR Amplify the DNA product...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of aquatic products, and discloses a biological inhibitor for silver carp surimi gel degradation. The inhibitor is a silver carp MBSP-specific shark-derived single-domain antibody, myofibrillar-binding serine protease (MBSP) which causes the silver carp surimi gel degradation is used as an antigen to immunize ginglymostoma cirratum, a single-domain antibody geneis separated from ginglymostoma cirratum serum, a single-domain antibody gene text library is established, a single-domain antibody target gene with high-specificity is obtained through the phage display technology, and then the silver carp MBSP-specific shark-derived single-domain antibody with high-purity, high-activity, and high-expression amount is separated and obtained by using the cloning expression technology and affinity chromatography technology. The biological inhibitor can significantly inhibit the activity of silver carp myofibrillar-binding serine protease, and effectively inhibits the gel degradation of silver carp surimi. The biological inhibitor involved in the invention is safe, specific, and efficient, has simple operation during the use process, and can be applied to the inhibition of the gel degradation of silver carp surimi.

Description

technical field [0001] The invention relates to the field of aquatic products, in particular to a biological inhibitor for silver carp surimi gel deterioration. Background technique [0002] Surimi products are an important direction of freshwater fish processing in my country, and gel deterioration is one of the main factors affecting the quality of surimi products today. The earliest research by Japanese scholars found that the myogen-binding serine protease (MBSP) in fish muscle tissue is the main reason for the decrease of gel strength caused by long-term heating of surimi products at 50-65 °C. MBSP can degrade myosin heavy chain in myofibrils under certain temperature conditions, and at the same time degrade α-actinin, actin and tropomyosin. Because MBSP widely exists in fish muscle tissue, it cannot be removed by rinsing during the production of surimi, which seriously restricts the development of surimi products industry. [0003] At present, research on MBSP inhibi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A23L17/60
CPCA23L17/65Y02A40/81
Inventor 隋建新
Owner OCEAN UNIV OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products