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New application of pyruvic acid

A technology of pyruvic acid and medicine, applied in the field of new uses of pyruvic acid, can solve problems such as no reports on new uses of pyruvic acid, and achieve the effects of alleviating pain and improving application prospects

Inactive Publication Date: 2020-06-12
YUEYANG INTEGRATED TRADITIONAL CHINESE & WESTERN MEDICINE HOSPITAL SHANGHAI UNIV OF CHINESE TRADITIONAL MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is no report about the new application of pyruvic acid of the present invention

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1 Pyruvate reduces the verification of UVB-induced human skin DNA damage

[0065] 1 Experimental method

[0066] Cyclobutane pyrimidine dimer (CPD) is one of the major premutational lesions induced by UV radiation. These lesions result in mutations and strongly promote malignant transformation of skin cancers, including nonmelanoma skin cancer and melanoma. To explore the role of exogenous pyruvate in preventing UVB-induced DNA damage, we evaluated pyruvate at 500J / m 2 The effect of dose of UVB irradiation on human foreskin specimens (human foreskin specimens were obtained from human foreskins discarded after circumcision of Urology Department of Yueyang Hospital of Integrated Traditional Chinese and Western Medicine affiliated to Shanghai University of Traditional Chinese Medicine). Treatment group at 500J / m 2 Pyruvate (1000ng) was injected intradermally 6 hours before ultraviolet irradiation, and the control group received only blank treatment. The epiderm...

Embodiment 2

[0071] Example 2 Verification that pyruvate has a protective effect on UVB-induced mouse skin DNA damage

[0072] 1 Experimental method

[0073] To investigate whether exogenous pyruvate had a similar protective effect on the epidermis of mice, we injected 500 mg / kg pyruvate intraperitoneally on the shaved back of mice or performed vehicle control. After 6 hours, all mice received 500J / m 2 UV exposure.

[0074] 2 Experimental results

[0075] The dorsal skin was collected 3 hours after UVB treatment and stained by using immunofluorescence ( figure 2 A) and immunodot blot ( figure 2 B) Measuring CPD levels to detect DNA damage. Similar to the results of human in vivo experiments, we also found that pyruvate treatment significantly reduced UVB-induced CPD levels in mouse epidermis.

[0076] 3 Conclusion

[0077] The results showed that pyruvate effectively protected mouse skin from UVB-induced DNA damage.

Embodiment 3

[0078] Example 3 Verification of the protective effect of pyruvate on DNA damage in human skin cells induced by UVB

[0079] 1 Experimental method

[0080] To further confirm the protective effect of pyruvate on UVB-induced DNA damage, we also performed similar experiments on human primary keratinocytes (HPK) and human primary melanocytes (HPM) (human primary keratinocytes ( HPK) and primary human melanocytes (HPM) were derived from HPK and HPM cells separated from the foreskin of a cooperative hospital). Cells were pre-incubated with pyruvate (1mM) for 3 hours, and then treated with 100J / m 2 of UVB irradiation. Cells were collected 5 minutes, 1 hour and 2 hours after UVB irradiation.

[0081] 2 Experimental results

[0082] Immunofluorescence staining( image 3 A) and ELISA ( image 3 B) The results consistently demonstrate the protective effect of pyruvate against UV-induced HPK DNA damage. Similar results for HPM ( Figure 4 A and 4B) also demonstrate the role of py...

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Abstract

The invention relates to the technical field of a new application of medicines, and particularly relates to a new application of a pyruvic acid. The pyruvic acid can protect human and mouse skins fromUVB-induced DNA damage, and experimental results show that the pyruvic acid is sufficient to reduce accumulation of UVB-induced CPD in human and mouse in-vivo models; and in addition, the pyruvic acid also protects primary keratinocytes and melanocytes from UVB-induced DNA damage. More importantly, an exogenous pyruvic acid significantly increases an acetylation level of histone H3-lysine 9 (H3K9), and directly participates in a nucleotide excision repair (NER) pathway. The invention also discloses the application of the pyruvic acid in preparation of drugs for resisting apoptosis induced byultraviolet UVB irradiation. Skin aging and an incidence rate of a skin cancer can be effectively reduced, and the pyruvic acid can be well applied to clinic, and has a good application prospect.

Description

technical field [0001] The invention relates to the technical field of new uses of medicines, in particular to the new uses of pyruvate. Background technique [0002] The skin is directly irradiated by ultraviolet rays from the sun, and excessive exposure will damage cellular tissues such as DNA, fat membrane, and mitochondria, thereby causing skin aging, apoptosis of related cells, and even canceration. Studies have shown that after ultraviolet radiation can induce a variety of skin damage, including photoaging, wrinkles, sunburn, cataract, visual damage, immune damage, various skin cancers such as basal cell carcinoma, squamous cell carcinoma and malignant melanoma, photosensitivity Various light-related skin diseases such as phototoxic reaction and actinic keratosis. Ultraviolet radiation (UVR) is electromagnetic radiation with a wavelength between 100 nm and 400 nm. It is divided into three different forms according to wavelength: UVA (320-400nm), UVB (280-320nm) and U...

Claims

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Application Information

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IPC IPC(8): A61K31/19A61P17/16A61P17/18A61P35/00A61P29/00
CPCA61K31/19A61P17/16A61P17/18A61P29/00A61P35/00
Inventor 韩昌鹏李斌李欣杨豪杰韩宜霖
Owner YUEYANG INTEGRATED TRADITIONAL CHINESE & WESTERN MEDICINE HOSPITAL SHANGHAI UNIV OF CHINESE TRADITIONAL MEDICINE
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