The method for doubling haplobiont of gerbera

A haploid and plant technology, applied in the field of plant biology, can solve the problems of toxicity and high price of chemical mutagens, and achieve the effects of high affinity, low price and reducing the dosage of drugs

Pending Publication Date: 2020-06-16
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems of high toxicity and high price of chemical mutagens existing in colchicine doubling haploids, t

Method used

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  • The method for doubling haplobiont of gerbera
  • The method for doubling haplobiont of gerbera
  • The method for doubling haplobiont of gerbera

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A method for doubling gerbera haploid plants, characterized in that it comprises the following steps:

[0044] A. Strong seedlings and proliferation culture

[0045] Cut the gerbera haploid tissue culture propagation seedlings into individual plants, and transfer them to the following strong seedling and proliferation medium for simultaneous cultivation of strong seedlings and proliferation:

[0046] MS Modified Medium

[0047]

[0048] The MS improved culture solution is: the concentration of macroelements in the MS culture solution is halved, the concentration of thiamine hydrochloride is 2 mg / L, and the concentration of pyridoxine hydrochloride is 1 mg / L;

[0049] The culture conditions are: light intensity 1800~2200lx, temperature 25℃±2℃, light time 10h / d;

[0050] The cultivation time is: 35d.

[0051] B. Chemical mutagenesis culture

[0052] Cut the seedlings cultivated in step A into individual plants with a seedling height of 0.8-1.2 cm, completely soak i...

Embodiment 2

[0073] Embodiment 2 is the same as Embodiment 1 except for the following operations, and will not be repeated here.

[0074] A. Strong seedlings and proliferation culture

[0075] The cultivation time is: 40d;

[0076] B. Chemical mutagenesis culture

[0077] Cut the seedlings cultivated in step A into individual plants with a seedling height of 0.8-1.2 cm, completely soak in the chemical mutagen, and treat for 4 hours. After the treatment, soak in sterile water for 20 minutes, and then rinse with sterile water for 3 times , inoculated into the following mutagenesis medium for culture:

[0078] 1 / 2MS medium

[0079]

[0080]

[0081] The preparation method of the mutagen mother solution is: first dissolve 0.5g amisulfameline in 50g dimethyl sulfoxide, then add distilled water to make the volume to 1L, to obtain the mutagen mother solution; the chemical mutagen The preparation method is: take the aforementioned 20ml mutagen mother solution, dilute it to 100ml with dis...

Embodiment 3

[0087] Embodiment 3 is the same as Embodiment 1 except for the following operations, and will not be repeated here.

[0088] A. Strong seedlings and proliferation culture

[0089] Culture time: 45d;

[0090] B. Chemical mutagenesis culture

[0091] Cut the seedlings cultivated in step A into individual plants with a seedling height of 0.8-1.2 cm, completely soak in the chemical mutagen, and treat for 3 hours. After the treatment, soak in sterile water for 20 minutes, and then rinse with sterile water for 3 times , inoculated into the following mutagenesis medium for culture:

[0092] 1 / 2MS medium

[0093]

[0094] The preparation method of the mutagen mother solution is: first dissolve 0.5g amisulfameline in 50g dimethyl sulfoxide, then add distilled water to make the volume to 1L, to obtain the mutagen mother solution; the chemical mutagen The preparation method is: take the aforementioned 30ml mutagen mother solution, dilute it to 100ml with distilled water, sterilize...

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Abstract

The invention discloses a method for doubling a haplobiont of gerbera. The method for doubling the haplobiont of the gerbera comprises the following steps: double haplobiont plants are finally determined when tissue-cultured breeding seedlings of the haplobiont of the gerbera go through strong seedlings and multiplication culture, chemical mutagenesis culture, rejuvenation culture, variant plantsscreening, breed conservation of primary plants, propagation, rooting culture, mutagenic strains reselection, identification of stomata guard cells chloroplast counting method, and identification of root tip chromosome counting method. The ploidy doubling cultivation of haplobiont of the gerbera and identification processes are optimized, while mutation rate of the haplobiont is increased, and theaccuracy of ploidy identification is guaranteed. The method for doubling the haplobiont of the gerbera can effectively reduce the formation and interference of chimeras, reduce the damage of chemicaldrugs to the plants to reduce death rate of the haplobiont, and keep variation rate of the haplobiont between 60-80%, and provides the double haplobiont with excellent traits and high stability for breeding of new variety of the gerbera.

Description

technical field [0001] The invention relates to a method for doubling gerbera haploid plants, belonging to the field of plant biotechnology. Background technique [0002] Gerbera Hybrida (Gerbera Hybrida) is a perennial herbaceous flower belonging to the genus Gerbera in the Compositae family. Abundant, it becomes an indispensable cut flower for anniversary supply. The heterosis between inbred lines is greater than that between varieties, and gerbera is a typical cross-pollination crop. Due to long-term asexual reproduction, it retains high heterozygosity of genotype, and its traits are extremely complex. [0003] In order to overcome the heterozygous state of gerbera varieties, make full use of heterosis, eliminate dominant and recessive interference, and improve the accuracy of selection, it is necessary to artificially control pollination and force selfing to improve the homozygosity of the parents. Usually, in order to obtain a homozygous inbred line, multiple generati...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01H1/08
CPCA01H4/008A01H4/001A01H1/08
Inventor 单芹丽李绅崇杨春梅瞿素萍吴丽芳吴学尉汪国鲜阮继伟卢珍红
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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