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Temperature-sensitive hydrogel compound suitable for carrying human umbilical cord Wharton's Jelly-derived mesenchymal stem cells and application of temperature-sensitive hydrogel compound

A temperature-sensitive hydrogel, Wharton's glue technology, applied in the field of biomedicine, can solve problems such as low stem cell survival rate

Inactive Publication Date: 2020-06-16
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is to overcome the low survival rate of human umbilical cord Wharton's jelly mesenchymal stem cells carried on existing thermosensitive hydrogels and the limited application of thermosensitive hydrogel complexes in the field of skin damage repair. Defects and deficiencies, providing a temperature-sensitive hydrogel complex carrying human umbilical cord Wharton's jelly mesenchymal stem cells, and adding sodium ascorbyl phosphate, which can effectively promote the growth of human umbilical cord Wharton's jelly mesenchymal stem cells Survival rate, promote cell migration ability, and have a better effect on promoting skin healing

Method used

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  • Temperature-sensitive hydrogel compound suitable for carrying human umbilical cord Wharton's Jelly-derived mesenchymal stem cells and application of temperature-sensitive hydrogel compound
  • Temperature-sensitive hydrogel compound suitable for carrying human umbilical cord Wharton's Jelly-derived mesenchymal stem cells and application of temperature-sensitive hydrogel compound
  • Temperature-sensitive hydrogel compound suitable for carrying human umbilical cord Wharton's Jelly-derived mesenchymal stem cells and application of temperature-sensitive hydrogel compound

Examples

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Effect test

Embodiment 1

[0054] A thermosensitive hydrogel complex comprising the following components:

[0055] Thermosensitive hydrogel PEO-PPO-PEO block copolymer PF-127, sodium ascorbyl phosphate and PBS buffer, wherein the concentration of sodium ascorbyl phosphate is 400 μM, and the mass volume fraction of PF-127 is 20%.

[0056] The preparation method of the above-mentioned thermosensitive hydrogel composite comprises the following steps:

[0057] 20% (w / v) PF-127 was used to carry P4 human umbilical cord Wharton's jelly mesenchymal stem cells, and then 400 μM sodium ascorbyl phosphate was added, and mixed evenly.

Embodiment 2

[0059] A thermosensitive hydrogel complex comprising the following components:

[0060] Thermosensitive hydrogel PEO-PPO-PEO block copolymer PF-127, sodium ascorbyl phosphate and PBS buffer, wherein the concentration of sodium ascorbyl phosphate is 800 μM, and the mass volume fraction of PF-127 is 20%.

[0061] The preparation method of the above-mentioned temperature-sensitive hydrogel composite is the same as that in Example 1.

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Abstract

The invention discloses a temperature-sensitive hydrogel compound suitable for carrying human umbilical cord Wharton's Jelly-derived mesenchymal stem cells (WJ-MSCs) and an application of the temperature-sensitive hydrogel compound. The temperature-sensitive hydrogel compound is prepared from the following raw materials in parts by weight: temperature-sensitive hydrogel, a temperature-sensitive hydrogel additive and a solvent, and the temperature-sensitive hydrogel additive is sodium ascorbyl phosphate. The temperature-sensitive hydrogel compound provided by the invention can significantly promote the survival rate of human umbilical cord Wharton's Jelly-derived mesenchymal stem cells and enhance the cell migration ability; the product can be used for repairing large-area mouse skin with whole cortex excision, and an obvious injury healing effect can be seen on the eighth day after transplantation; the temperature-sensitive hydrogel can be used as a temperature-sensitive hydrogel medicinal preparation, and is applied to the fields of promoting survival of carried cells, cell migration, tissue repair, tissue regeneration and the like.

Description

technical field [0001] The invention relates to the technical field of biomedicine, more specifically, to a temperature-sensitive hydrogel compound suitable for carrying human umbilical cord Wharton's jelly mesenchymal stem cells and its application. Background technique [0002] Umbilical cord Wharton's jelly mesenchymal stem cells have good self-renewal ability, multiple differentiation potentials and immune regulation functions, so they are widely used in regeneration and repair projects of various tissues, such as nervous system diseases, skin injury healing and regeneration, etc. Wait. [0003] Cell embedding is one of the key technologies when transplanting cells in tissue regeneration engineering. By using a certain embedding agent (solvent), the transplanted cells are encapsulated in it, so that they are relatively isolated from the target tissue in terms of immunity, and can be implanted Maintaining the concentration of transplanted cells close to the target tissue...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K9/06A61K47/10A61K47/34A61K47/22A61P17/02
CPCA61K9/06A61K35/28A61K47/10A61K47/22A61K47/34A61P17/02
Inventor 黄军就邓庆吒
Owner SUN YAT SEN UNIV
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