Bacteriophage-containing spray type environment disinfectant as well as preparation method and application thereof
A technology of bacteriophage and disinfectant, which is applied in the field of spray-type environmental disinfectant and its preparation, can solve the problems of low phage titer, narrow application range, and short storage time, and achieve the goal of overcoming corrosive and irritating odors and wide application range , strong specific effect
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Embodiment 1
[0032] Preparation of bacteriophage disinfectant
[0033] After the purchased phages were preserved and purified in our laboratory, they were activated, dissolved in PBS and diluted to make the concentration of each phage ≥10 10 pfu / ml, and the concentrations of the three bacteriophages are equal, and then the other auxiliary materials are prepared to the corresponding concentration with ultrapure water, and then mixed with various auxiliary materials according to the following volume ratio, and mixed evenly, to obtain a disinfectant. The formula composition is as follows:
[0034] Coliphage CCTCC NO.M2016539 20%;
[0035] Staphylococcus aureus phage CCTCC NO.M2016535 20%;
[0036] Hemolytic streptococcal phage CCTCC NO.M2019227 20%;
[0037] 0.003M sodium dodecylsulfonate (SDS) solution 20%;
[0038] SM buffer 10%;
[0039] 0.1M Sucrose 10%.
[0040] Wherein, the preparation method of SM buffer comprises the following steps: adding 0.1g gelatin, 2g MgSO 4 ·7H 2 O and 5...
Embodiment 2
[0042] After the purchased phages were preserved and purified in our laboratory, they were activated, dissolved in PBS and diluted to make the concentration of each phage ≥10 10 pfu / ml, and the concentrations of the three bacteriophages are equal, and then the other auxiliary materials are prepared to the corresponding concentration with ultrapure water, and then mixed with various auxiliary materials according to the following volume ratio, and mixed evenly, to obtain a disinfectant. The formula composition is as follows:
[0043] Coliphage CCTCC NO.M2016539 10%;
[0044] Staphylococcus aureus phage CCTCC NO.M2016535 30%;
[0045] Hemolytic streptococcal phage CCTCC NO.M2019227 20%;
[0046] 0.003M sodium dodecylsulfonate (SDS) solution 20%;
[0047] SM buffer 10%;
[0048] 0.1M Sucrose 10%.
[0049] Wherein, the preparation method of SM buffer comprises the following steps: adding 0.1g gelatin, 2g MgSO 4 ·7H 2 O and 5.8 g NaCl.
Embodiment 3
[0051] After the purchased phages were preserved and purified in our laboratory, they were activated, dissolved in PBS and diluted to make the concentration of each phage equal to 10 9 pfu / ml, and then prepare other auxiliary materials to the corresponding concentration with ultrapure water, add various auxiliary materials according to the following volume ratio and mix them, and mix evenly to obtain a disinfectant. The formula composition is as follows:
[0052] Coliphage CCTCC NO.M2016539 20%;
[0053] Staphylococcus aureus phage CCTCC NO.M2016535 20%;
[0054] Hemolytic streptococcal phage CCTCC NO.M2019227 20%;
[0055] 0.003M sodium dodecylsulfonate (SDS) solution 20%;
[0056] SM buffer 10%;
[0057] 0.1M Sucrose 10%.
[0058] Wherein, the preparation method of SM buffer comprises the following steps: adding 0.1g gelatin, 2g MgSO 4 ·7H 2 O and 5.8 g NaCl.
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